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pDEST-GBKT7

Plasmid

#248655

Purpose

Empty vector for generating yeast 2-hybrid GAL4 DNA binding domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 binding domain, Myc

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-GADT7

Plasmid

#248657

Purpose

Empty vector for generating yeast 2-hybrid GAL4 activation domain fusion constructs by Gateway cloning. It may also be used as an empty vector experimental control.

Depositor

Sarah Assmann, John Doonan

Article

Rossignol et al J Cell Sci. 2007 Oct 15;120(Pt 20):3678-

Type

Empty backbone

Use

Yeast 2-hybrid

Tags

GAL4 activation domain, HA

Expression

Yeast

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pDEST-ITGB1-V1

Plasmid

#215454

Purpose

Expression vector with integrin beta1 tagged with the BiFC V1 fragment

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

ITGB1 (ITGB1 Human)

Tags

Venus fragment 1 (V1)

Expression

Mammalian

Mutation

Silent mutations added to disrupt shRNA binding a…

Promoter

CMV

Available Since

Nov. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-YPet

Plasmid

#215541

Purpose

Expression vector with the YPet fluorescent protein

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

YPet

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-PTPN11

Plasmid

#215523

Purpose

Expression vector with Shp2

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pDEST-Dok1-V2

Plasmid

#215452

Purpose

Expression vector with Dok1 tagged with the BiFC V2 fragment

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Tags

Venus fragment 2 (V2)

Expression

Mammalian

Promoter

CMV

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pEF.DEST51-Dok1

Plasmid

#215458

Purpose

Expression vector with Dok1

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

DOK1 (DOK1 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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SRGAP2C_pEF-DEST51

Plasmid

#238497

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

SRGAP2C (SRGAP2C Human)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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ARHGAP11B_pEF-DEST51

Plasmid

#238498

Purpose

IVT mRNA of human gene

Depositor

Megan Dennis

Article

Soto et al bioRxiv [Preprint]. 2024 Sep 26:2024.09.

Insert

ARHGAP11B (ARHGAP11B Human)

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pDEST-TDP-43-HA

Plasmid

#235496

Purpose

Expression of human TDP-43-HA fusion protein

Depositor

Maxime Rousseaux

Article

Suk et al Mol Neurodegener. 2025 Mar 28;20(1):38.

Insert

TARDBP (TARDBP Human)

Tags

HA

Expression

Mammalian

Available Since

April 30, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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