We narrowed to 14,886 results for: pET

1. pET-51b(+) ChemoG3

   Plasmid

   #193801

   Purpose

   Protein production of ChemoG3 in bacteria

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG3
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   EGFP:A206K; HT7: L271E

   Promoter

   T7

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-51b(+) ChemoG4

   Plasmid

   #193804

   Purpose

   Protein production of ChemoG4 in bacteria

   Depositor

   Julien Hiblot

   Article

   Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

   Insert

   ChemoG4
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   EGFP:A206K; HT7: E143R, E147R, L271E

   Promoter

   T7

   Available since

   June 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET24b-6His-fSNAP

   Plasmid

   #106999

   Purpose

   To express 6His-fSNAP protein in E.coli

   Depositor

   Melissa Moore

   Article

   Haraszti et al Methods Mol Biol. 2020;2113:17-22. doi:

   Insert

   fSNAP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-32a(+)-TGFB3m

   Plasmid

   #120289

   Purpose

   Produce human recombinant mTGFB3 in DE3 cells

   Depositor

   Paul Burridge

   Article

   Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

   Insert

   monomeric Transforming Growth Factor-β3
   

   Use

   Tags

   6x His and S-Tag

   Expression

   Bacterial

   Mutation

   Codon optimized for E. Coli production

   Promoter

   T7 Promoter

   Available since

   June 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PET.SUMO eIF4G1 1-200

   Plasmid

   #158791

   Purpose

   Bacterial expression vector for the expression of N-terminal 6xHIS-SUMO-tagged codon optimized eIF4G1 amino acids 1-200 isoform lacking the microexon.

   Depositor

   Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

   Insert

   eIF4G1 (codon optimized)
   

   Use

   Tags

   6xHIS and SUMO

   Expression

   Bacterial

   Mutation

   amino acids 1-200

   Promoter

   T7

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET24a-LaM4-3xTS

   Plasmid

   #162777

   Purpose

   Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitope

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   anti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
   

   Use

   Tags

   BAP, HA, His6, T7, and TS site from proCCK

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET22b-BirA-CPDSalI

   Plasmid

   #38258

   Depositor

   Aimee Shen, Matthew Bogyo

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   birA (birA E. coli)
   

   Use

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pGEX4T1-BirA

   Promoter

   T7

   Available since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pET28b-Ets1-ETS

   Plasmid

   #85735

   Purpose

   Expresses DNA-binding (ETS) domain of murine Ets-1, residues 311 to 440

   Depositor

   Gregory Poon

   Article

   Wang et al J Biol Chem. 2014 Aug 1;289(31):21605-16

   Insert

   DNA-binding (ETS) domain of murine Ets-1, residues 331 to 440 (Ets1 Human, Mouse)
   

   Use

   Tags

   6xHis following thrombin cleavage site LVPR|G

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-MSP1D1deltaH4/2H5

   Plasmid

   #71715

   Purpose

   Membrane scaffold protein (MSP), helix 4/2 + helix5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Brg1 Del2 pET28a

    Plasmid

    #122116

    Purpose

    Expression Brg1 amino acids 1-837 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    6XHis and His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21b-LOV-ipaA

    Plasmid

    #40236

    Depositor

    Brian Kuhlman

    Article

    Lungu et al Chem Biol. 2012 Apr 20;19(4):507-17.

    Insert

    LOV-ipaA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Jα helix region hybridized (AsLOV2 aa 537-546/ipa…

    Promoter

    T7

    Available since

    Sept. 4, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pET28a-mH6-Cas12i2

    Plasmid

    #120883

    Purpose

    Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.

    Depositor

    Arbor Biotechnologies

    Article

    Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

    Insert

    Cas12i2
    

    Use

    CRISPR

    Tags

    mH6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    lac

    Available since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET-CVm-PTEN

    Plasmid

    #168032

    Purpose

    Expresses eCFP-PTEN(M265-N329)-mVenus in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    eCFP-PTEN(M265-N329)-mVenus
    

    Use

    Tags

    Hisx6 and myc

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. CBP-HAT-pET

    Plasmid

    #21091

    Depositor

    Corey Largman

    Article

    Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

    Insert

    CBP (Crebbp Mouse)
    

    Use

    Tags

    His and T7 tag

    Expression

    Bacterial

    Mutation

    HAT domain aa 1171-1621

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pET-22b(+)-HpENR

    Plasmid

    #12651

    Depositor

    SeWon Suh

    Article

    Lee et al Acta Crystallogr D Biol Crystallogr. 200

    Insert

    noyl-acyl carrier protein reductase (fabI Helicobacter pylori)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-51b(+)-Caprola_14

    Plasmid

    #194675

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_14 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_14
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET-51b(+)-Caprola_15

    Plasmid

    #194676

    Purpose

    Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Caprola_15
    

    Use

    Tags

    His-tag and Strep-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET29b-ClbPpep-CHis

    Plasmid

    #48243

    Purpose

    First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

    Depositor

    Emily Balskus

    Article

    Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

    Insert

    ClbP-pep
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    First 375 amino acids of the peptidase, which con…

    Promoter

    T7

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-baPrs-hdNadV

    Plasmid

    #91950

    Purpose

    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

    Depositor

    George Cătălin Marinescu

    Article

    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

    Inserts

    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed Leucine 135 to Isoleucine

    Promoter

    T7

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a(His10-PD-1cyto)

    Plasmid

    #177896

    Purpose

    E coli expression of His10 tag fused to human PD-1 Intracellular Domain

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    PD-1 (aa 194-288 only) (PDCD1 Human)
    

    Use

    Tags

    10xHis-Thrombin cut site-T7 tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge