Skip to main content
Addgene
Showing: 5351 - 5400 of 9184 results

  4. pSEVA3b67Rb

    Plasmid
    #58311
    Purpose
    Shuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.
    Depositor
    Tom Ellis
    Article
    Wright et al ACS Synth Biol. 2014 May 13.
    Insert
    mRFP1
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    58311-plasmid-map-sequence-id-89824

    Enlarge

  13. pET28-6His-SUMO-Nsp10

    Plasmid
    #154959
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154959-plasmid-map-sequence-id-307848

    Enlarge

  15. WT FL USP7

    Plasmid
    #227214
    Purpose
    Expresses human USP7 with a N-terminal cleavable 6His tag in E. coli
    Depositor
    Irina Bezsonova
    Insert
    USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
    Use
    Tags
    6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7-promoter
    Available since
    Nov. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    227214-plasmid-map-sequence-id-452331

    Enlarge

  20. pET28-6His-TEV-Nsp14

    Plasmid
    #154954
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-TEV-Nsp14 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154954-plasmid-map-sequence-id-307815

    Enlarge

  21. pCMK188

    Plasmid
    #226659
    Purpose
    pCB2.4 - pBR322 shuttle vector that replicates in E. coli and Synechococcus sp. PCC 7002, contains HIP1 site
    Depositor
    David Nielsen
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    226659-plasmid-map-sequence-id-455439

    Enlarge

  24. pET22-Nsp10-TEV-6His

    Plasmid
    #154957
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp10-TEV-6His (ORF1ab SARS-CoV-2)
    Use
    Tags
    TEV-6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154957-plasmid-map-sequence-id-306327

    Enlarge

  29. pG1C

    Plasmid
    #71740
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71740-plasmid-map-sequence-id-130141

    Enlarge

  30. pGEX4T-3-6AOptParkin

    Plasmid
    #165107
    Purpose
    bacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin gene
    Depositor
    Arthur Haas
    Insert
    Parkin isoform 1 (PRKN Human)
    Use
    Tags
    GST-6-alanine
    Expression
    Bacterial
    Mutation
    codon optimized for E. coli expression
    Promoter
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165107-plasmid-map-sequence-id-326710

    Enlarge

  31. pTGD

    Plasmid
    #88894
    Purpose
    Tandem gene duplication cassette for RecA mediated homologous recombination in E. coli
    Depositor
    Keith Tyo
    Article
    Love et al Methods Mol Biol. 2019;1927:37-45. doi:
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    88894-plasmid-map-sequence-id-174381

    Enlarge

  32. pET28-Nsp14-TEV-6His

    Plasmid
    #154952
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable 6His tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp14-TEV-6His (ORF1ab SARS-CoV-2)
    Use
    Tags
    TEV-6His
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154952-plasmid-map-sequence-id-307820

    Enlarge

  34. PPDK

    Plasmid
    #64037
    Purpose
    Expression of Pyruvate phosphate dikinase (PPDK) in E. coli
    Depositor
    Vern Schramm
    Article
    Sturm et al Anal Chem. 2009 Apr 15;81(8):2847-53. do
    Insert
    pyruvate phosphate dikinase
    Use
    Tags
    none
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pET28-6His-SUMO-Nsp14

    Plasmid
    #154956
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp14 (ORF1ab SARS-CoV-2)
    Use
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154956-plasmid-map-sequence-id-307845

    Enlarge

  40. pG1AK

    Plasmid
    #71736
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Article
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Type
    Empty backbone
    Use
    Synthetic Biology; Shuttle vector
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71736-plasmid-map-sequence-id-130104

    Enlarge

  44. pRL488

    Plasmid
    #70263
    Purpose
    designed to facilitate transcriptional fusions to luxAB, encoding luciferase, on a plasmid that can replicate in both Escherichia coli and strains of filamentous cyanobacteria.
    Depositor
    Peter Wolk
    Article
    Elhai et al EMBO J. 1990 Oct;9(10):3379-88.
    Type
    Empty backbone
    Use
    Luciferase; Cyanobacteria expression
    Tags
    luxAB
    Expression
    Mutation
    Promoter
    none
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    70263-plasmid-map-sequence-id-130206

    Enlarge

  48. pGEX6P1 pS43 METTL3

    Plasmid
    #200732
    Purpose
    Bacterial expression of GST-Tagged codon optimized human pS43 METTL3
    Depositor
    Claudio Alarcón
    Insert
    pS43 METTL3 (METTL3 Human)
    Use
    Tags
    GST from backbone plasmid
    Expression
    Bacterial
    Mutation
    Codon optimized for expression in E. coli, UAG co…
    Promoter
    Available since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    200732-plasmid-map-sequence-id-401809

    Enlarge

Showing: 5351 - 5400 of 9184 results