We narrowed to 14,579 results for: pET

1. pET28_6xHis-PARP1-A762V

   Plasmid

   #174795

   Purpose

   Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A762V (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A762V

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBP-pET-RBS

   Plasmid

   #72981

   Purpose

   PET RBS - derived from pET vectors (5' GTAC/3' CATA fusion)

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   pET RBS derived from pET vectors
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pETM6-3F2-mCherry

   Plasmid

   #73418

   Purpose

   Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   Promoter 3F2 (orthogonal T7-lac variant)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P3F2 (orthogonal T7-lac variant)

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-PLS-KP2_rpoA-U

   Plasmid

   #190956

   Purpose

   Test the U-to-C editing activity of KP2 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-PLS-KP3_rpoA-U

   Plasmid

   #190960

   Purpose

   Test the U-to-C editing activity of KP3 on AtrpoA editing site, in E. coli

   Depositor

   Bernard Gutmann

   Article

   Ichinose et al Commun Biol. 2022 Sep 15;5(1):968. doi:

   Insert

   PLS-KP3
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET12a-KIo18/08 C214S

   Plasmid

   #47099

   Depositor

   Marina Ramirez-Alvarado

   Insert

   KIo18/08 C214S (IGKV3-20 Human)
   

   Use

   Tags

   ompT

   Expression

   Bacterial

   Mutation

   C214S

   Promoter

   T7

   Available since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pET28b-trAtCCD8(pYL736)

   Plasmid

   #178287

   Purpose

   Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

   Depositor

   Yanran Li

   Article

   Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

   Insert

   trAtCCD8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-PKM2-H391Y

   Plasmid

   #122692

   Purpose

   Express His-tagged PKM2 (H391Y) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   H391Y

   Promoter

   Available since

   March 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pETCON-IgG1/design_50

   Plasmid

   #30301

   Depositor

   David Baker

   Article

   Fleishman et al J Mol Biol. 2011 Sep 29.

   Insert

   IgG Fc Designed Binder IgG1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 8, 2011

   Availability

   Academic Institutions and Nonprofits only

10. pET28a His6Flag2AUF1(p42)

    Plasmid

    #135941

    Purpose

    Expresses AUF1p42 in E. coli

    Depositor

    Yimon Aye

    Article

    Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

    Insert

    heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)
    

    Use

    Tags

    Flag2 and His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSHDY*in_PpetE:BCD2:mVenus

    Plasmid

    #133970

    Purpose

    Heterologous, copper-inducible expression of the reporter fluorophor mVenus in Synechocystis sp. PCC 6803. Used as backbone for cloning of GOI downstream of the PpetE:BCD2 expression module.

    Depositor

    Pia Lindberg

    Article

    Dienst et al Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.

    Insert

    mVenus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    inserted gene is codon optimized for Synechocyst…

    Promoter

    PpetE:BCD2

    Available since

    April 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-SpyTag002 DA-MBP

    Plasmid

    #102832

    Purpose

    Bacterial expression of unreactive version of SpyTag002 fused to maltose binding protein

    Depositor

    Mark Howarth

    Article

    Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

    Insert

    SpyTag002 DA-MBP
    

    Use

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Reactive aspartic acid has been mutated to an ala…

    Promoter

    T7

    Available since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-At_PRORP1-His

    Plasmid

    #67869

    Purpose

    bacterial expression of PRORP1 (At2g32230), full coding sequence (mitoch./chloropl. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP1 (PRORP1 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Residues 36-572 (see comments)

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7CFE1-TelR15-YPet

    Plasmid

    #49646

    Purpose

    TALEYellow-Telomere for In Vitro Translation

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

    Insert

    TelR15
    

    Use

    In vitro translation

    Tags

    YPet

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-28a(+)-HMGS

    Plasmid

    #108945

    Purpose

    heterologous expression of HMGS of E. faecalis in E. coli

    Depositor

    Frank Schulz

    Article

    Schulz et al Chembiochem. 2018 Aug 7. doi: 10.1002/cb

    Insert

    HMG-CoA synthase (HMGS)
    

    Use

    Tags

    His6-Tag

    Expression

    Bacterial

    Mutation

    A110G

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-ZZ(1700-1751)

    Plasmid

    #99334

    Purpose

    Bacterial expression of mouse CBP ZZ domain

    Depositor

    Peter Wright

    Article

    Legge et al J Mol Biol. 2004 Oct 29;343(4):1081-93.

    Insert

    CBP ZZ domain (Crebbp Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. ymScarletI-YPET pDRF1-GW

    Plasmid

    #168063

    Purpose

    FRET fusion between ymScarletI and YPET for use in yeast

    Depositor

    Bas Teusink

    Insert

    ymScarletI-YPET
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET32a-His-FADD

    Plasmid

    #78803

    Purpose

    To purify His-tagged recombinant FADD protein

    Depositor

    Jaewhan Song

    Insert

    GIG3 (FADD Human)
    

    Use

    Tags

    6x His, S-Tag, Thrombin cleavage site, and TrxA (…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pET-Cdc12-Cdc11

    Plasmid

    #131162

    Purpose

    Expression of Septin rods

    Depositor

    Nils Johnsson

    Article

    Renz et al BMC Biotechnol. 2013 Jul 26;13:60. doi:

    Insert

    6his-Cdc12 + Cdc11
    

    Use

    Bicistronic

    Tags

    Cdc11: S-Tag and Cdc12: 6his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET21-pelB-LaG-2

    Plasmid

    #172739

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-2; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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