We narrowed to 14,886 results for: pET

1. pET28a-PKM2-G415R

   Plasmid

   #122693

   Purpose

   Express His-tagged PKM2 (G415R) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   G415R

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-24a-CXCR4

   Plasmid

   #133431

   Purpose

   Human CXCR4 coding sequence in vector for in vitro transcription and protein synthesis, with T7 promoter.

   Depositor

   Gerhard Wagner

   Insert

   CXCR4 (CXCR4 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_6xHis-PARP1delWGR-L713F

   Plasmid

   #173941

   Purpose

   Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delWGR-L713F (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 521-660; L713F, V762A

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21-pelB-LaG-27

   Plasmid

   #172752

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-27; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-27
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET3-ARL15-His6

   Plasmid

   #201880

   Purpose

   Used for bacterial expression of human protein ARL15

   Depositor

   Richard Kahn

   Insert

   ARL15 (ARL15 Human)
   

   Use

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-p53 (1-73) (72P)

   Plasmid

   #62083

   Purpose

   Expression of human p53 (1-73) (72P) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   HIs

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; 72P

   Promoter

   T7

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pET28a_6HT-TBP6.7(R49A)

   Plasmid

   #112726

   Purpose

   Arg49 of TBP6.7 and other TBPs is the only arginine that was lab-evolved from wild-type U1A to play a significant role in TAR RNA recognition. Mutation results in ~230-fold reduction in Kd.

   Depositor

   Joseph Wedekind

   Article

   Belashov et al Nucleic Acids Res. 2018 Jul 27;46(13):64

   Insert

   6His-TEV-TBP6.7(R49A)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   R49A

   Promoter

   T7

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pet 3d alpha 1 beta 2 CP

   Plasmid

   #13450

   Depositor

   John Cooper

   Article

   Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

   Insert

   capping protein alpha 1 beta 2 (mouse) (Capza1 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   Dec. 15, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pET28b(+)-SPN+IFS

   Plasmid

   #83372

   Purpose

   Expresses SPN and IFS complex in E. coli cell

   Depositor

   SeWon Suh

   Article

   Yoon et al J Synchrotron Radiat. 2013 Nov;20(Pt 6):

   Inserts

   SPN
   IFS
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. eGFP-Silicatein-pET28

    Plasmid

    #198012

    Purpose

    C-terminal eGFP fusion to truncated silicatein insert with N- and C-terminal hexahistidine tag for purification

    Depositor

    Bryan Berger

    Article

    Vigil et al Materials Advances 2, 2023

    Insert

    eGFP-silicatein
    

    Use

    Tags

    hexahistidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2

    Plasmid

    #116953

    Purpose

    Expresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readout

    Depositor

    Randall Platt

    Article

    Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

    Insert

    Fusicatenibacter saccharivorans RT-Cas1-Cas2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-Beclin1 (1-242)

    Plasmid

    #24401

    Depositor

    Qing Zhong

    Article

    Sun et al Proc Natl Acad Sci U S A. 2008 Dec 9. 10

    Insert

    Beclin1 (BECN1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains amino acids 1-242 only.

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pET28-MRPP3-His

    Plasmid

    #67866

    Purpose

    bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Vilardo et al Nucleic Acids Res. 2012 Dec;40(22):11583

    Insert

    MRPP3 (KIAA0391 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino acid 46 of recombinant MRPP3 is preceded by…

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21a- hPTP1e-PDZ2

    Plasmid

    #20377

    Depositor

    Andrew Lee

    Article

    Hu et al Biochemistry. 2003 Dec 2. 42(47):13856-6

    Insert

    hPTP1e PDZ2 (PTPN13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET20b-GpNLuc-6xHis

    Plasmid

    #135932

    Purpose

    Expresses the GpNLuc LumiFluor in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET3a-UBE2J1 1-283

    Plasmid

    #185348

    Purpose

    In vitro transcription and translation in bacterial system

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    UBE2J1 (UBE2J1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Cytoplasmic domain only, aa 1-283

    Promoter

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM33_ZYMND11_coiled-coil MYND

    Plasmid

    #178493

    Purpose

    Bacterial expression of human domain with His-tag and GST tag

    Depositor

    Ylva Ivarsson

    Article

    Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

    Insert

    ZYMND11_coiled-coil MYND (ZMYND11 Human, Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Promoter

    T7/LacO

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-PesaR-Clux

    Plasmid

    #47658

    Purpose

    PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Synth Biol. 2013 Jul 23.

    Insert

    PesaR-C
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Additional esa box between -35 site and the origi…

    Promoter

    PesaR-C

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pET30a-SRAP-delCys2

    Plasmid

    #84568

    Purpose

    Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    Srap1 (Hmces Mouse)
    

    Use

    Tags

    6xHis and S-Tag

    Expression

    Bacterial

    Mutation

    deletion of Cys2

    Promoter

    T7

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET20b-delta133HtrA2 A141S

    Plasmid

    #16157

    Depositor

    L. Miguel Martins

    Article

    Strauss et al Hum Mol Genet. 2005 Aug 1. 14(15):2099-1

    Insert

    HtrA2 (HTRA2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    A141S

    Promoter

    Available since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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