pET11d_Rev
(Plasmid
#119322)
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PurposeExpression of protein Rev from HIV
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 119322 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET11d
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Backbone manufacturerNovagen
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameRev
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SpeciesHIV B1
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Entrez Generev (a.k.a. HIV1gp6)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 FW
- 3′ sequencing primer T7 RV (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET11d_Rev was a gift from Francisco Blanco & Robert Tycko (Addgene plasmid # 119322 ; http://n2t.net/addgene:119322 ; RRID:Addgene_119322) -
For your References section:
Solid-state NMR data support a helix-loop-helix structural model for the N-terminal half of HIV-1 Rev in fibrillar form. Blanco FJ, Hess S, Pannell LK, Rizzo NW, Tycko R. J Mol Biol. 2001 Nov 2;313(4):845-59. doi: 10.1006/jmbi.2001.5067. 10.1006/jmbi.2001.5067 PubMed 11697908
