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Degron-KI p53 donor vector

Plasmid

#65619

Purpose

Donor vector to generate Degron-KI DD-p53

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

p53 Degron-KI left and right HR arms

Use

Tags

Expression

Mutation

Promoter

Available since

July 13, 2015

Availability

Academic Institutions and Nonprofits only
JP1520 PIK3CA E545K HA

Plasmid

#14571

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA E545K (PIK3CA Bovine)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

E545K

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
JP1520 PIK3CA H1047R HA

Plasmid

#14572

Depositor

Joan Brugge

Article

Isakoff et al Cancer Res. 2005 Dec 1. 65(23):10992-100

Insert

PIK3CA H1047R (PIK3CA Bovine)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

H1047R

Promoter

Available since

April 12, 2007

Availability

Academic Institutions and Nonprofits only
Degron-KI ezh2 donor vector

Plasmid

#65620

Purpose

Donor vector to generate Degron-KI DD-EZH2

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

EZH2 left and right HR arms

Use

Tags

Expression

Mutation

Promoter

Available since

July 24, 2015

Availability

Academic Institutions and Nonprofits only
Degron-KI PIK3CA donor vector

Plasmid

#65621

Purpose

Donor vector to generate Degron-KI DD-PIK3CA

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

PIK3CA Degron-KI left and right HR arms

Use

Tags

Expression

Mutation

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-rB50

Plasmid

#18957

Depositor

Mariano Viapiano

Article

Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

Insert

Brevican N-terminus (Bcan Rat)

Use

Tags

Expression

Mammalian

Mutation

Brevican N-terminus, aminoacids 1-395

Promoter

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
Luke-2

Plasmid

#228900

Purpose

Luke-2 localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFP

Depositor

John Samuelson

Article

Kanakapura Sundararaj et al mSphere. 2024 Sep 25;9(9):e0046624. doi:

Insert

Carbohydrate binding domain cbm49 protein

Use

Acanthamoeba expression vector

Tags

eGFP

Expression

Mutation

none

Promoter

Own

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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Jonah-1

Plasmid

#228899

Purpose

Jonah-1 localizes to the outer cyst wall of Acanthamoeba tagged with eGFP

Depositor

John Samuelson

Article

Kanakapura Sundararaj et al mSphere. 2024 Sep 25;9(9):e0046624. doi:

Insert

Jonah-1

Use

Acanthamoeba expression vector

Tags

eGFP

Expression

Mutation

none

Promoter

Own promoter

Available since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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Leo-A

Plasmid

#228901

Purpose

Leo-A localizes to the inner cyst wall and ostioles of Acanthamoeba tagged with eGFP

Depositor

John Samuelson

Article

Kanakapura Sundararaj et al mSphere. 2024 Sep 25;9(9):e0046624. doi:

Insert

Leo-A

Use

Acanthamoeba expression vector

Tags

eGFP

Expression

Mutation

none

Promoter

Own

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pADH118-2

Plasmid

#90984

Purpose

NAT-marked C. albicans ADE2-specific gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.

Depositor

Aaron Hernday

Article

Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

Insert

NAT 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. albicans LEU2 2 of 2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pRetroX-Tight-MCS_PGK-GpNLuc

Plasmid

#70185

Purpose

Constiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects..

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRetroX-Tight-MCS_PGK-OgNLuc

Plasmid

#70186

Purpose

Constiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.

Depositor

Antonio Amelio

Article

Schaub et al Cancer Res. 2015 Sep 30. pii: canres.353

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-Teton-puro-shFAK #1

Plasmid

#198757

Purpose

conditional knockdown of FAK

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

Insert

shFAK #1 (PTK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-Teton-puro-shFAK #2

Plasmid

#198758

Purpose

conditional knockdown of FAK

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

Insert

shFAK #2 (PTK2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK-caCas9-NatMX-Neut5L-Leu2 drive

Plasmid

#89578

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Leu2 gene drive

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4-rB90

Plasmid

#18959

Depositor

Mariano Viapiano

Article

Viapiano et al J Neurooncol. 2008 Jul . 88(3):261-72.

Insert

Brevican C-terminus (Bcan Rat)

Use

Tags

Expression

Mammalian

Mutation

Rat brevican C-terminus: Signal peptide aa 1-22 …

Promoter

Available since

Dec. 12, 2008

Availability

Academic Institutions and Nonprofits only
pJK-caCas9-NatMX-Neut5L-Ade2 drive

Plasmid

#89577

Purpose

caCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locus

Depositor

George Church

Article

Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

Insert

Ade2 gene drive

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-MYC-As-NF-kB3

Plasmid

#180205

Purpose

Expresses MYC-tagged Acanthoeca spectabilis NF-kB3 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB3

Use

Tags

MYC-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-412

Promoter

CMV

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-As-NF-kB2

Plasmid

#180201

Purpose

Expresses FLAG-tagged Acanthoeca spectabilis NF-kB2 protein in mammalian cells

Depositor

Thomas Gilmore

Article

Williams et al Commun Biol. 2021 Dec 16;4(1):1404. doi:

Insert

As-NF-kB2

Use

Tags

FLAG-tagged fusion protein

Expression

Mammalian

Mutation

aa 2-502

Promoter

CMV

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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