We narrowed to 4,689 results for: crispr c plasmids

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pJA46

Plasmid

#59928

Purpose

gRNA for cleavage at rde-1(D801) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D801) gRNA

Use

CRISPR

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pJA45

Plasmid

#59927

Purpose

gRNA for cleavage at rde-1(D718) locus in C elegans

Depositor

Andrew Fire

Article

Arribere et al Genetics. 2014 Aug 26. pii: genetics.114

Insert

rde-1(D718) gRNA

Use

CRISPR

Promoter

U6

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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v3em-Cterm-PE2max-∆RNaseH-dualU6

Plasmid

#198735

Purpose

AAV genome encoding C-terminal PE2max and U6 expression cassettes

Depositor

David Liu

Article

Davis et al Nat Biotechnol. 2023 May 4. doi: 10.1038

Insert

NpuC-CtermPE2max∆RNaseH

Use

AAV

Promoter

Cbh

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA1-NLS(sv40) (BPK5050)

Plasmid

#115136

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA2.1-NLS(sv40) (BPK5059)

Plasmid

#115137

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2.1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2.1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA3.1-NLS(sv40) (RTW2624)

Plasmid

#115139

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3.1 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3.1 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA3-NLS(sv40) (BPK5077)

Plasmid

#115140

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA3 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA3 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hAcrVA2-NLS(sv40) (AAS2283)

Plasmid

#115138

Purpose

CMV-T7 promoter expression plasmid for human codon optimized AcrVA2 anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized AcrVA2 anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-hOrf2mor-NLS(sv40) (BPK5095)

Plasmid

#115141

Purpose

CMV-T7 promoter expression plasmid for human codon optimized Orf2mor anti-CRISPR protein with C-terminal NLS

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized Orf2mor anti-CRISPR protein

Tags

NLS(SV40)

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pCX420

Plasmid

#229211

Purpose

Cytoplasmic-targeting Csm complex plasmid

Depositor

Jennifer Doudna

Article

Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

Insert

Csm1; Csm2; Csm3(RNase mut)-2xsfGFP; Csm4; Csm5; Cas6

Expression

Mammalian

Available Since

Feb. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAP973-1

Plasmid

#99496

Purpose

TEV::linker::meGFP:3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::meGFP::3XFlag

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP974-1

Plasmid

#99497

Purpose

TEV::linker::tagRFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagRFP::3XFlag

Expression

Bacterial

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAP997-1

Plasmid

#99500

Purpose

3XFlag::tagRFP::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagRFP::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP975-1

Plasmid

#99498

Purpose

TEV::linker::tagBFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::linker::tagBFP::3XFlag

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAP758-1

Plasmid

#99494

Purpose

TEV::eGFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::3XFlag

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP546-2

Plasmid

#99476

Purpose

3XFlag::mNeptune::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::mNeptune::Myc

Expression

Bacterial

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pAP693-3

Plasmid

#99492

Purpose

nirFP::3xFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

nirFP::3xFlag

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAP998-5

Plasmid

#99501

Purpose

3XFlag::tagBFP::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagBFP::linker::TEV

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP576-2

Plasmid

#99481

Purpose

3XFlag::tagBFP::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::tagBFP::Myc

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pX330_REC8_cterm

Plasmid

#222522

Purpose

Cas9/sgRNA plasmid for targeting REC8

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2024.05.31.596483

Insert

Cas9, REC8 sgRNA (REC8 Human, Synthetic)

Use

CRISPR

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Gfap-KO

Plasmid

#196489

Purpose

To KO Gfap in mouse astrocytes

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Insert

gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Gfap-GeneTRAP

Plasmid

#196492

Purpose

To KO and genetrap mouse GFAP simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGEARBOCS-v0-Vamp2-GeneTRAP

Plasmid

#196493

Purpose

To KO and genetrap mouse VAMP2 simultaneously

Depositor

Cagla Eroglu

Article

Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

Inserts

mCherry-CAAX
gRNA

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAP574-1

Plasmid

#99477

Purpose

3XFlag::Topaz::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::Topaz::Myc

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAP996-1

Plasmid

#99499

Purpose

3XFlag::meGFP::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP::linker::TEV

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAP835-1

Plasmid

#99495

Purpose

TEV::meGFP::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::meGFP::3XFlag

Expression

Bacterial

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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HK1 gRNA (BRDN0001145157)

Plasmid

#77643

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK1 (HK1, HOOK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HK1 gRNA (BRDN0001147564)

Plasmid

#77644

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK1 (HK1, HOOK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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HK1 gRNA (BRDN0001146994)

Plasmid

#77645

Purpose

3rd generation lentiviral gRNA plasmid targeting human HK1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

HK1 (HK1, HOOK1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pH-STEME-NG-esgRNA

Plasmid

#138136

Purpose

Targeted simultaneous C-to-T and A-to-G in rice

Depositor

Caixia Gao

Insert

APOBEC3A-wtTadA-TadA7.10-nCas9-NG-UGI-NLS

Expression

Plant

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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