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Showing: 5451 - 5500 of 9184 results

  1. pET28-eIF4E-TwStrep

    Plasmid
    #208638
    Purpose
    for E. coli expression of C-ter Twin Strep tagged human eIF4E (ENST00000450253)
    Depositor
    Quanfu Ma
    Insert
    human eIF4E (ENST00000450253) (EIF4E Human)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208638-plasmid-map-sequence-id-415918

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  2. pET28-mEGFP(A206K)-TwStrep

    Plasmid
    #208622
    Purpose
    for E. coli expression of C-ter Twin Strep tagged mEGFP(A206K), EGFP cloned from pEGFP-C1, A206K introduced by seamless (Gibson) clone
    Depositor
    Quanfu Ma
    Insert
    mEGFP(A206K)
    Use
    Tags
    6xHis and Twin Strep
    Expression
    Bacterial
    Mutation
    (compared with pEGFP-C1, protein level) A206K
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208622-plasmid-map-sequence-id-415916

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  8. pSG09-OR2-OR1-Pr-sigma54

    Plasmid
    #102466
    Purpose
    Expresses sigma 54 protein in E. coli. The expression is controlled by a OR2-OR1-Pr promoter.
    Depositor
    Richard Murray
    Article
    Guo et al bioRxiv 122606
    Insert
    sigma 54 (rpoN )
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    OR2-OR1-Pr
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    102466-plasmid-map-sequence-id-197263

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  21. pHL 886

    Plasmid
    #64907
    Purpose
    KanR + ColE1 + PLlacO-1::RBS(st2):lacZ::RBS(st7):cfp::T1 terminator
    Depositor
    Han Lim
    Article
    Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8
    Type
    Empty backbone
    Use
    Synthetic Biology; E. coli
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  24. pET32bplus_trx_dAChE4

    Plasmid
    #83917
    Purpose
    Encodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coli
    Depositor
    Sarel Fleishman
    Article
    Goldenzweig et al Mol Cell. 2016 Jul 21;63(2):337-46. doi:
    Insert
    Designed hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
    Use
    Tags
    His, S-tag, and TRX
    Expression
    Bacterial
    Mutation
    V13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…
    Promoter
    T7 promoter
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    83917-plasmid-map-sequence-id-174928

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  25. pAAV-syn-FLP-FRT-iGluSnFR4s-NGR-WPRE

    Plasmid
    #234453
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4s-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234453-plasmid-map-sequence-id-465748

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  26. pK27Sumo_His-SUMO-nsp10-nsp16 fusion (SARS-CoV-2)

    Plasmid
    #169191
    Purpose
    To express nsp10-nsp16 fusion protein in E. coli
    Depositor
    John Diffley
    Article
    Basu et al Biochem J. 2021 Jul 16;478(13):2481-2497
    Insert
    14His-SUMO-nsp10-GSGSGS-nsp16 (ORF1ab SARS-CoV-2, Synthetic)
    Use
    Tags
    14His-SUMO and Fusion protein of SARS-CoV-2 nsp10…
    Expression
    Bacterial
    Mutation
    Codon optimised for E. coli
    Promoter
    T5
    Available since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169191-plasmid-map-sequence-id-333984

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  32. pAAV-syn-FLP-FRT-iGluSnFR4f-NGR-WPRE

    Plasmid
    #234454
    Purpose
    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.
    Depositor
    GENIE Project
    Article
    Aggarwal et al bioRxiv 2025.03.20.643984
    Insert
    iGluSnFR4f-NGR
    Use
    AAV; Flp/frt
    Tags
    IgK chain and Myc epi tag-NGR GPI anchor
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234454-plasmid-map-sequence-id-465691

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  40. pIMAY

    Plasmid
    #68939
    Purpose
    E. coli/staphylococcal temperature-sensitive plasmid for allelic exchange
    Depositor
    Tim Foster
    Article
    Monk et al MBio. 2012 Mar 20;3(2). pii: e00277-11.
    Type
    Empty backbone
    Use
    Staphylococcal allelic exchange vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    68939-plasmid-map-sequence-id-210239

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  42. pSAP

    Plasmid
    #206429
    Purpose
    A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    206429-plasmid-map-sequence-id-419733

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  43. pL97

    Plasmid
    #78576
    Purpose
    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains strong constitutive ermEp promoter
    Depositor
    Xuming Mao
    Article
    Sun et al Annals of Microbiology Dec 2012, Volume
    Type
    Empty backbone
    Use
    Streptomyces expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    ermEp* promoter from S. erythraea
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    78576-plasmid-map-sequence-id-421926

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  45. pL99

    Plasmid
    #78577
    Purpose
    Over-expression shuttle vector for gene cloning and transfer from E. coli to Streptomyces. Contains inducible PnitA-NitR system
    Depositor
    Xuming Mao
    Article
    Sun et al Annals of Microbiology Dec 2012, Volume
    Type
    Empty backbone
    Use
    Streptomyces expression
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    inducible PnitA-NitR system
    Available since
    May 27, 2016
    Availability
    Academic Institutions and Nonprofits only
    78577-plasmid-map-sequence-id-263121

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Showing: 5451 - 5500 of 9184 results