We narrowed to 18,091 results for: multi

1. p5xNF-kb_RE

   Plasmid

   #118050

   Purpose

   GBPart - Operator/DNA Response element - 5 copies of the NF-kb DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   5 copies of the NF-kb DNA binding motif
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. hCMV-IE1:ELuc

   Plasmid

   #118062

   Purpose

   Transcriptional unit for constitutive expression of ELuc in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:ELuc:bGH
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Expression

   Mammalian

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. p2xp53_RE

   Plasmid

   #118053

   Purpose

   GBPart - Operator/DNA Response element - 2 copies of the p53 DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   2 copies of the p53 DNA binding motif
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p4xSMAD_RE

   Plasmid

   #118051

   Purpose

   GBPart - Operator/DNA Response element - 4 copies the SMAD DNA binding motif

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   4 copies the SMAD DNA binding motif
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Expression

   Mammalian

   Available Since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMMK ENTR 4 CMV eGFP

   Plasmid

   #206272

   Purpose

   ENTR Vector 4 for MultiSite Gateway assembly. Encodes eGFP under the control of a CMV promoter

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   eGFP
   

   Use

   Multimate/gateway entr 4

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMMK ENTR 4 CMV mTagBFP

   Plasmid

   #206279

   Purpose

   ENTR Vector 4 for MultiSite Gateway assembly. Encodes mTagBFP under the control of a CMV promoter.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   mTagBFP
   

   Use

   Multimate/gateway entr 4

   Expression

   Mammalian

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR-mcBEST

   Plasmid

   #209416

   Purpose

   Plasmid for multiplexed cytosine base editing in streptomycetes

   Depositor

   Tilmann Weber

   Article

   Whitford et al ACS Synth Biol. 2023 Aug 18;12(8):2353-2

   Inserts

   Codon optimized APOBEC1-nCas9-UGI fusion protein
   csy4 from Pseudomonas aeruginosa PAO1
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   base editing cassette: PtipA ; sgRNAs: PkasO* an…

   Available Since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

   Plasmid

   #176627

   Purpose

   TF B self-activation cassette in MultiFate-2.2 and MultiFate-3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

   Plasmid

   #176626

   Purpose

   TF A self-activation cassette in MultiFate-2.2 and MultiFate-3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMMK ENTR 2 hU6 ACTB sgRNA

    Plasmid

    #206270

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNA

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    ACTB sgRNA
    

    Use

    CRISPR; Multimate/gateway entr 2

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMMK ENTR 2 hU6 HEK3 PegRNA

    Plasmid

    #206277

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    HEK3 PegRNA
    

    Use

    CRISPR; Multimate/gateway entr 2

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMMK ENTR 3 polH aeBlue polH VSV-G

    Plasmid

    #206278

    Purpose

    ENTR Vector 3 for MultiSite Gateway assembly. Encodes aeBlue chromoprotein and VSV-G glycoprotein under the control of viral polH promoters.

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    aeBlue, VSV-G
    

    Use

    Multimate/gateway entr 3

    Expression

    Bacterial and Insect

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAC8_MF-P10-mCherry-pH-3C-TwinStrep (VE5621)

    Plasmid

    #139770

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter TwinStrep tag under the PH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal TwinStrep tag
    

    Tags

    3C - TwinStrep tag

    Promoter

    PH

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAC8_MF-p10-mCh PH-mCh (VE5625)

    Plasmid

    #139769

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    mCherry fluorescent protein
    

    Expression

    Insect

    Promoter

    PH or p10

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMMK ENTR 1 HITI-2c ACTB

    Plasmid

    #206269

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    HITI-2c donor
    

    Use

    CRISPR; Multimate/gateway entr 1

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMMK ENTR 2 CMV mKO2-hCdt1

    Plasmid

    #206282

    Purpose

    ENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMV

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mKO2-hCdt1 (CDT1 Human)
    

    Use

    Multimate/gateway entr 2

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMMK ENTR 1 CMV mAG h-Geminin

    Plasmid

    #206281

    Purpose

    ENTR Vector 1 for MultiSite Gateway assembly. Encodes mAG-hGeminin under the control of CMV

    Depositor

    Imre Berger

    Article

    Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    mAG-hGeminin (GMNN Human)
    

    Use

    Multimate/gateway entr 1

    Expression

    Mammalian

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. MLRV2: NF-kb-SMAD-DBE-P53-AP1

    Plasmid

    #124536

    Purpose

    Multiplex luciferase reporter vector, with luciferase reporters for the pathways NFKB, SMAD, FOXO, P53 and AP1

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Inserts

    5 copies of the NF-kb DNA binding motif
    7xSMAD_RE::FLuc
    3xDBE_BE::Renilla (FOXO)
    2xP53_RE::NLuc
    6xAP-1_RE::GrRenilla
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MLRV3:HRE-P53-MAPK/JNK-FOXO-TGFβ

    Plasmid

    #178320

    Purpose

    Multiplex luciferase reporter vector, with luciferase reporters for the pathways HRE, P53, AP-1, FOXO and SMAD.

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Methods Mol Biol. 2022;2524:409-432. doi

    Inserts

    HRE RedFirefly reporter
    P53 FLuc reporter
    AP-1 Renilla reporter
    FOXO NLuc Reporter
    SMAD GrRenilla reporter
    

    Use

    Luciferase and Synthetic Biology

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLCA.66/2272

    Plasmid

    #22733

    Depositor

    Mark Magnuson

    Article

    Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

    Insert

    Lox66-pU(delta)TK-EM7Neo-Lox2272
    

    Use

    Cre/Lox; Bac recombineering

    Mutation

    This vector was designed for assembling gene targ…

    Available Since

    Dec. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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