3,636 results

1. pAFMW-Nbr[C399-625 CM]

   Plasmid

   #188588

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler C-terminal domain, catalytic mutant
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   D435A [GAT=>GCC]; E435A [GAA=>GCC]

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAFMW-Nbr[C399-625]-Gwpep(Mm)

   Plasmid

   #188589

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
   

   Use

   Tags

   FLAG-Myc and GWpep mm

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAFMW-Nbr[K276A]

   Plasmid

   #188597

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   K276A [AAG=>GCG]

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAFMW-Nbr[C399-625]-Gwpep(Dm)

   Plasmid

   #188590

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
   

   Use

   Tags

   FLAG-Myc and Gwpep dm

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAFMW-Gwpep(Dm)-Nbr[C399-625]

   Plasmid

   #188592

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
   

   Use

   Tags

   FLAG-Myc-Gwpep dm

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAFMW-Gwpep(Mm)-Nbr[C399-625]

   Plasmid

   #188591

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   GW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
   

   Use

   Tags

   FLAG-Myc-Gwpep mm

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAFMW-Nbr[R272A,K276A]

   Plasmid

   #188596

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   R272A [CGG=> GCG], K276A [AAG=>GCG]

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAFMW-Nbr[K284A]

   Plasmid

   #188595

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   K284A [AAG=> GCG]

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAFMW-Nbr[R272A]

   Plasmid

   #188594

   Purpose

   Flag-tagged Nibbler variants expression in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

   Insert

   Nibbler (Nbr Fly)
   

   Use

   Tags

   FLAG-Myc

   Expression

   Insect

   Mutation

   R272A [CGG=> GCG]

   Promoter

   Available since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAFMW-Nbr[K187A]

    Plasmid

    #188593

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    K187A [AAA=> GCA]

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAFMW-Nbr-CM

    Plasmid

    #188585

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler Catalytic mutant
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG; D435A [GAT=>GCC]; E435A [GA…

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAFMW-Nbr

    Plasmid

    #188584

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler (Nbr Fly)
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAFMW-Nbr[N1-383]

    Plasmid

    #188586

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler N-terminal domain
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    deleted first ATG

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAFMW-Nbr[C399-625]

    Plasmid

    #188587

    Purpose

    Flag-tagged Nibbler variants expression in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Xie et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

    Insert

    Nibbler C-terminal domain
    

    Use

    Tags

    FLAG-Myc

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc-sgRNA-Cas9.scr_1

    Plasmid

    #190703

    Purpose

    Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Scrambled sgRNA 1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc-sgRNA-Cas9.scr_3

    Plasmid

    #190705

    Purpose

    Expresses scrambled sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Scrambled sgRNA 3
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Drosophila U6

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. HA-doraWT_rescue_construct

    Plasmid

    #190608

    Purpose

    Puromycin-selectable expression of HA-tagged Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. dora[B]_rescue_construct

    Plasmid

    #190637

    Purpose

    Puromycin-selectable expression of Dora[T295M] (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Changed threonine 295 to methionine

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-dora[B]_rescue_construct

    Plasmid

    #190638

    Purpose

    Puromycin-selectable expression of HA-tagged Dora[T295M] (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    3xHA

    Expression

    Insect

    Mutation

    Changed threonine 295 to methionine

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. dora[A]_rescue_construct

    Plasmid

    #190639

    Purpose

    Puromycin-selectable expression of truncated Dora (CG34401) in Drosophila S2 cells

    Depositor

    David Bartel

    Article

    Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

    Insert

    Dorado (CG34401 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Truncated to contain amino acids 1-945

    Promoter

    Actin-5c

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge