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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 10,632 results for: t7

Showing: 2201 - 2220 of 10632 results

  9. pRSF:His6-CylA-27-412

    Plasmid
    #241275
    Purpose
    For expression of the Enterococcal cytolysin activating protease CylA
    Depositor
    Wilfred van der Donk
    Insert
    CylA protease
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    N-terminal secretion signal sequence removed (res…
    Promoter
    T7
    Available Since
    Sept. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    241275-plasmid-map-sequence-id-487216

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  10. SARS2_HisSUMOTEV-nsp7/nsp8

    Plasmid
    #234405
    Purpose
    Bacterial expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Inserts
    Nsp7_SARS2
    Nsp8_SARS2
    Tags
    His6-SUMO-TEV
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234405-plasmid-map-sequence-id-469465

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  12. pAIP4_3CX2

    Plasmid
    #234221
    Purpose
    Heterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3CX2 (MYBPC3 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234221-plasmid-map-sequence-id-464186

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  13. pAIP4_4ACJ

    Plasmid
    #234212
    Purpose
    Heterologous protein expression of Wufb25h12 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4ACJ (oxr1a Zebrafish)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234212-plasmid-map-sequence-id-464177

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  14. pAIP4_1EYH

    Plasmid
    #234159
    Purpose
    Heterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EYH (Epn1 Rat)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234159-plasmid-map-sequence-id-464125

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  15. pET28a_HCK

    Plasmid
    #224239
    Purpose
    Expression of human HCK Kinase in E. coli
    Depositor
    Andrew Mesecar
    Insert
    hck (HCK Human)
    Tags
    6xHis and 6xHis-TEV
    Expression
    Bacterial
    Promoter
    T7 promotor
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    224239-plasmid-map-sequence-id-459258

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  16. CPH3736_GST-TEV-Spt6p-tSH2-1223-1454

    Plasmid
    #227088
    Purpose
    Bacterial expression of R1282H/K1355A/K1435A triple-mutant GST-tagged Spt6 tSH2 domain (1223-1454)
    Depositor
    Chris Hill
    Insert
    spt6-tsh2 (SPT6 Budding Yeast)
    Tags
    GST-TEV_cleavage_site
    Expression
    Bacterial
    Mutation
    R1282H, K1355A, K1435A
    Promoter
    T7
    Available Since
    Nov. 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    227088-plasmid-map-sequence-id-451126

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  17. pET29b(+)_gp1(A11V)

    Plasmid
    #225131
    Purpose
    Phage GIL01 protein gp1 (A11V) mutant with a single nucleotide substitution (C32T)
    Depositor
    Matej Butala
    Insert
    gp1 (A11V)
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Alanine 11 to Valine
    Promoter
    T7 promoter
    Available Since
    Oct. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    225131-plasmid-map-sequence-id-448443

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  18. pET-29b(+)_notufA-CmEndoCOM_3C-CBD-His

    Plasmid
    #218198
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Cordyceps militaris EndoCOM
    Depositor
    Florence Pojer
    Insert
    EndoCOM
    Tags
    3C-CBD-His
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 19-315
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218198-plasmid-map-sequence-id-431794

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  19. pT7CFE1_polio_VP12A

    Plasmid
    #212988
    Purpose
    Encodes Poliovirus type I Mahoney VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    Poliovirus type I Mahoney VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA tag
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212988-plasmid-map-sequence-id-420361

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  20. pT7CFE1_EVD68_VP12A

    Plasmid
    #212984
    Purpose
    Encodes EV-D68 MO/14/18947 VP12A optimized for expression in mammalian transcription/translation extracts
    Depositor
    Karla Kirkegaard
    Insert
    EV-D68 MO/14/18947 VP12A
    Use
    Mammalian cell free protein expression
    Tags
    HA
    Promoter
    T7
    Available Since
    Jan. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    212984-plasmid-map-sequence-id-420357

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Showing: 2201 - 2220 of 10632 results