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Showing: 5551 - 5600 of 38145 results
  1. pAAV-Syn-FLEX-rc[ChrimsonR-tdTomato]

    Plasmid
    #62723
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal. TdTomato is a codon diversified version.
    Depositor
    Has Service
    AAV1 and AAV5
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available since
    April 30, 2015
    Availability
    Academic Institutions and Nonprofits only
  2. pAAV-EF1α1.1-ChrimsonR-tdTomato

    Plasmid
    #122064
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α promoter (1.1kb short version).tdTomato has codons varied to reduce recombination. Using SV40 pA signal.
    Depositor
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1a(1.1kb short version)
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pAAV-Syn-FLEX-fd [ChrimsonR-tdTomato]

    Plasmid
    #84483
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the Syn promoter, in floxed/forward (Cre-dependent) manner. Cre turns gene off. Using bGHpA signal. tdTomato is a codon diversified version.
    Depositor
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    human synapsin promoter
    Available since
    April 26, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. AAV-EF1α1.1-FLPX-rc [ChrimsonR-tdTomato]

    Plasmid
    #128587
    Purpose
    AAV-mediated expression of ChrimsonR-tdTomato under the EF1α1.1 promoter in frt/reversed (Flp-dependent) manner. tdTomato has codons varied to reduce recombination.
    Depositor
    Insert
    ChrimsonR-tdTomato
    Use
    AAV
    Tags
    tdTomato (codon diversified version)
    Expression
    Mammalian
    Mutation
    Chrimson K176R mutant
    Promoter
    EF1α (1.1 kb short version)
    Available since
    Sept. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. A3Bi-ctd E255A -Cas9n-UGI-NLS

    Plasmid
    #109429
    Purpose
    Expresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.
    Depositor
    Insert
    Apolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Insertion of an L1 intron into the coding sequenc…
    Promoter
    CMV
    Available since
    July 2, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pB-CuR-hSOD1

    Plasmid
    #232477
    Purpose
    The inducible PiggyBac Cumate Switch vector (PBQM812A-1 System Biosciences) expressing human SOD1gene including flag -tag (DYKDDDDK) on the 3' end
    Depositor
    Insert
    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-CuO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pB-TR-hSOD1

    Plasmid
    #232478
    Purpose
    Tetracycline inducible PiggyBac vector expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end
    Depositor
    Insert
    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-TetO
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. PGL4.11-COL1A1mTEAD

    Plasmid
    #230916
    Purpose
    Comparing with wild type COL1A1 promoter, tested whether mutant TEAD binding sites changed the promoter activity.
    Depositor
    Insert
    Human COL1A1 promoter with mutant TEAD elements (COL1A1 Human)
    Use
    Luciferase
    Tags
    Luciferase- luc2p
    Expression
    Bacterial and Mammalian
    Mutation
    mutant potential TEAD bind elements AGGAAT to CTG…
    Promoter
    COL1A1
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
  9. pcDNA3.1/hFGFR3-K650M-V5

    Plasmid
    #214909
    Purpose
    expression of the K650M mutant variant of human FGFR3, which is associated with Severe Achondroplasia with Developmental Delay and Acanthosis Nigricans (SADDAN)
    Depositor
    Insert
    FGFR3 (FGFR3 Human)
    Use
    Tags
    V5/His
    Expression
    Mammalian
    Mutation
    K650M substitution
    Promoter
    CMV
    Available since
    March 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  10. pKANAC3K

    Plasmid
    #221545
    Purpose
    Activation tagging for plant
    Depositor
    Type
    Empty backbone
    Use
    Activation tagging in plant
    Tags
    Expression
    Mutation
    Promoter
    Cauliflower mosaic virus 35S
    Available since
    Sept. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. pcDNA3.3_SARS2_Mu

    Plasmid
    #177578
    Purpose
    expressing SARS-CoV-2 B.1.621 (Mu) spike protein for pseudovirus production
    Depositor
    Insert
    Spike (Mu variant) (S SARS-CoV-2)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    T95I, Y144T, Y145S, R346K, E484K, N501Y, D614G, …
    Promoter
    CMV
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
  12. pAdeasy-CMV-AE-EF1α-R2

    Plasmid
    #194928
    Purpose
    expressing two fusion proteins of Ag85B-ESAT6 and Rv2031c-Rv2626c from multi-stage antigens of Mycobacterium tuberculosis
    Depositor
    Inserts
    AE
    EEF1A1 (EEF1A1 Human)
    R2
    Use
    Adenoviral
    Tags
    Ag85B-ESAT6, EF1a, and Rv2031c-Rv2626c
    Expression
    Mutation
    a 48 bp linker (Gly3ser)4 was added between the t…
    Promoter
    CMV and EF1a
    Available since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
  13. pSG5 Large T K1

    Plasmid
    #9055
    Depositor
    Insert
    large T antigen K1 (SV40gp6 simian virus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E107K. Mutation in pRb LxCxE binding domain.
    Promoter
    Available since
    Dec. 7, 2005
    Availability
    Academic Institutions and Nonprofits only
  14. pORFE1001

    Plasmid
    #112079
    Purpose
    Overexpression of the AtCAS9 in plant under 2x35S promoter
    Insert
    AtCAS9
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized Cas9 S. pyogenes
    Promoter
    2x35S
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  15. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. SV40 1: pBSSVD2005

    Plasmid
    #21826
    Depositor
    Insert
    SV40 Large T (SV40gp6 simian virus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    likely expresses aa109-708 of the large T antigen
    Promoter
    Available since
    Sept. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
  17. pSG5 Large T delta 434-444

    Plasmid
    #9054
    Depositor
    Insert
    large T antigen d434-444 (SV40gp6 simian virus)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deletion in part of the bipartite p53 binding dom…
    Promoter
    Available since
    Nov. 3, 2005
    Availability
    Academic Institutions and Nonprofits only
  18. pRT006.3 L1 dual-luciferase AI retrotransposition reporter

    Plasmid
    #213031
    Purpose
    Bi-directional luciferase antisense intron (firefly fluc AI) human LINE-1 retrotransposition reporter (ORFeus-Hs sequence) for sleeping beauty integration
    Insert
    Tet-On Human LINE-1 (ORFeus-Hs) Firefly + Renilla Luciferase Antisense Intron dual retrotransposition marker
    Use
    Luciferase
    Tags
    Firefly luciferase antisense intron (3' UTR)
    Expression
    Mammalian
    Mutation
    Promoter
    pTRE bidirectional tet-on
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  19. AbVec_IGKC

    Plasmid
    #183702
    Purpose
    Encode human Ig kappa chain constant region. For cloning variable region and mAb expression.
    Depositor
    Insert
    IGKC (IGKC )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
  20. AbVec_IGLC

    Plasmid
    #183703
    Purpose
    Encode human Ig lambda chain constant region. For cloning variable region and mAb expression.
    Depositor
    Insert
    IGLC2 (IGLC2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SP6
    Available since
    April 4, 2022
    Availability
    Academic Institutions and Nonprofits only
  21. pCAGGS-S-B.1.1.529-FLAG

    Plasmid
    #180774
    Purpose
    B.1.1.529 variant S-protein with c-terminal FLAG tag
    Depositor
    Insert
    S-protein B.1.1.529
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 23, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
  22. gWiz_BM40-vMAT1-VL-hIgG1-CL

    Plasmid
    #218441
    Purpose
    mammalian expression plasmid for the light chain fragment of an anti-vMAT1 Fab domain
    Depositor
    Insert
    vMAT1-VL-hIgG1-CL
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  23. gWiz_BM40-vGLUT1-VL-hIgG1-CL

    Plasmid
    #218439
    Purpose
    mammalian expression plasmid for the light chain fragment of an anti-vGLUT1 Fab domain
    Depositor
    Insert
    vGLUT1-VL-hIgG1-CL
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  24. gWiz_BM40-vMAT2-VL-hIgG1-CL

    Plasmid
    #218443
    Purpose
    mammalian expression plasmid for the light chain fragment of an anti-vMAT2 Fab domain
    Depositor
    Insert
    vMAT2-VL-hIgG1-CL
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  25. NOVA1 eGFP

    Plasmid
    #61275
    Purpose
    GFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1
    Insert
    NOVA1 (NOVA1 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  26. pET28a-mxLOX1

    Plasmid
    #104975
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Insert
    MXAN_1745 (MXAN_1745 Myxococcus xanthus)
    Use
    Tags
    6 histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  27. pET28a-mxLOX2

    Plasmid
    #104976
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Insert
    MXAN_1744 (MXAN_1744 Myxococcus xanthus)
    Use
    Tags
    6 histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Feb. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  28. INT_Cargo_T7_gutB

    Plasmid
    #212139
    Purpose
    Cargo plasmid for integrating PT7 expression cassette of xylitol dehydrogenase in in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Insert
    xylitol dehydrogenase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  29. PgyrA_gfp

    Plasmid
    #212146
    Purpose
    Plasmid expressing green fluorescent protein under constitutive promoter PgyrA control.
    Depositor
    Insert
    green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  30. pET28a-mxEH

    Plasmid
    #104977
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Insert
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Use
    Tags
    6 histidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
  31. pSBBi-GB CaXMT1

    Plasmid
    #139168
    Purpose
    SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes.
    Depositor
    Insert
    7-methylxanthosine synthase 1 [Coffea arabica]
    Use
    Transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α
    Available since
    Availability
    Academic Institutions and Nonprofits only
  32. pCT02

    Plasmid
    #212152
    Purpose
    Plasmid expressing 6x His tagged FAST-PETase under inducible promoter PT7 control.
    Depositor
    Insert
    FAST-PETase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    S121E, D186H, R224Q, N233K, R280A
    Promoter
    PT7
    Available since
    March 7, 2024
    Availability
    Academic Institutions and Nonprofits only
  33. INT_Cargo_PgyrA_gfp

    Plasmid
    #212138
    Purpose
    Cargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Insert
    green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  34. PHB1

    Plasmid
    #212153
    Purpose
    For PHB production in E. coli (PT7-pMB1).
    Depositor
    Insert
    poly(3-hydroxyalkanoate) polymerase subunit PhaC, PHA-specific beta-ketothiolase, acetoacetyl-CoA reductase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  35. pACYCduet-mxLOX1-EH

    Plasmid
    #104978
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Inserts
    MXAN_1745 (MXAN_1745 Myxococcus xanthus)
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7promoter
    Available since
    Feb. 8, 2018
    Availability
    Academic Institutions and Nonprofits only
  36. gWiz_BM40-vAchT-Cytosolic-VL-hIgG1-CL

    Plasmid
    #218447
    Purpose
    mammalian expression plasmid for the light chain fragment of an anti-vAchT Fab domain binding to the cytosolic side of the transporter
    Depositor
    Insert
    vAchT-Cytosolic-VL-hIgG1-CL
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
  37. gWiz_BM40-vGLUT1-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218438
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vGLUT1 Fab domain
    Depositor
    Insert
    vGLUT1-Vh-hIgG1-CH2
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
  38. gWiz_BM40-vMAT1-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218440
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vMAT1 Fab domain
    Depositor
    Insert
    vMAT1-Vh-hIgG1-CH3
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  39. gWiz_BM40-vMAT2-VH-hIgG1-CH1-TS-HIS

    Plasmid
    #218442
    Purpose
    mammalian expression plasmid for the heavy chain fragment of an anti-vMAT2 Fab domain
    Depositor
    Insert
    vMAT2-Vh-hIgG1-CH4
    Use
    Affinity Reagent/ Antibody
    Tags
    BM40 and TwinStrep-6xHis
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 25, 2024
    Availability
    Academic Institutions and Nonprofits only
  40. pCT03

    Plasmid
    #212150
    Purpose
    Plasmid expressing alanine dehydrogenase under inducible promoter PT7 control. The enzyme is thermophilic and works best at 50 C.
    Depositor
    Insert
    alanine dehydrogenase
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    PT7
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
  41. mBeta4/pCNA3.1

    Plasmid
    #197316
    Purpose
    Plasmid expressing an antigen used to generate an antibody that targets the BK beta4 potassium channel subunit
    Depositor
    Insert
    Slo B4 (Kcnmb4 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
  42. pACYCduet-mxLOX2-EH

    Plasmid
    #104979
    Purpose
    Biosynthesis of oxylipins by microbial enzymes
    Depositor
    Inserts
    MXAN_1744 (MXAN_1744 Myxococcus xanthus)
    MXAN_1644 (MXAN_1644 Myxococcus xanthus)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    May 18, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 5551 - 5600 of 38145 results