We narrowed to 2,341 results for: PUC19

1. pIG-602_NY-ESO-1_TCR_RFP

   Plasmid

   #207483

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   RFP, NY-ESO-1_TCR
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. AICSDP-4:TUBA1B-mEGFP

   Plasmid

   #87421

   Purpose

   Homology arms and linker-mEGFP sequence for N-terminus (second exon) tagging of human TUBA1B

   Depositor

   Allen Institute for Cell Science

   Article

   Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2

   Insert

   TUBA1B Homology Arms with mEGFP-linker (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   March 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pIG-641 JE-TRAC-BCMA(BB)28z-P2A

   Plasmid

   #186127

   Purpose

   BCMA-CAR DNA production

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   BCMA-CAR (TNFRSF17 Human)
   

   Use

   CRISPR

   Mutation

   WT

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJW1586

   Plasmid

   #121057

   Purpose

   mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mKate2-T^SEC^AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFLAG, C. elegans codon optimized mKate2, and au…

   Expression

   Worm

   Available Since

   June 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIG-747_CD19-28z_CAR_TFAP4

   Plasmid

   #207489

   Purpose

   This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

   Depositor

   Alexander Marson

   Article

   Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

   Insert

   TFAP4, CD19-28z_CAR (TFAP4 Human)
   

   Use

   CRISPR

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJW1595

   Plasmid

   #121062

   Purpose

   mKate2^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   mKate2^SEC^BioTag::AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

   Expression

   Worm

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJW1592

   Plasmid

   #121059

   Purpose

   GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   GFP^SEC^BioTag::AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

   Expression

   Worm

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJW1594

   Plasmid

   #121061

   Purpose

   TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   TagRFP-T^SEC^BioTag::AID*::3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

   Expression

   Worm

   Available Since

   June 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDV21 [pSNG-1::SNG-1::CRY2(D387A)olig(535)::SL2::mCherry]

   Plasmid

   #197599

   Purpose

   Pan-neuronal expression of SNG-1::CRY2(D387A)olig(535) in neurons of C. elegans. CRY2(D387A) is photoinactive

   Depositor

   Alexander Gottschalk

   Article

   Vettkotter et al Nat Commun. 2022 Dec 19;13(1):7827. doi:

   Insert

   Synaptogyrin-CRY2 (D387A+E490G + residues 1-535) (CRY2, sng-1 Mustard Weed, Nematode)
   

   Expression

   Worm

   Mutation

   D387A, E490G

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIG-858_CD19-BBz_CAR_TFAP4

    Plasmid

    #207503

    Purpose

    This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    TFAP4, CD19-BBz_CAR (TFAP4 Human)
    

    Use

    CRISPR

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTR-459 tNGFR-P2A-STAT6

    Plasmid

    #186070

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    STAT6 (STAT6 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTR-460 tNGFR-P2A-STAT4

    Plasmid

    #186075

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    STAT4 (STAT4 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTR-458 tNGFR-P2A-STAT2

    Plasmid

    #186076

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    STAT2 (STAT2 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTR-455 tNGFR-P2A-WAS

    Plasmid

    #186082

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    WAS (WAS Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. P521_VSX2_p2A-h2b_mRuby3

    Plasmid

    #239104

    Purpose

    Used in CRISPR gene editing to Insert a p2A-h2b_mRuby3 sequence before the stop codon of the endogenous human VSX2 gene

    Depositor

    Karl Wahlin

    Article

    Jones et al Cells. 2022 Oct 28;11(21):3412. doi: 10.

    Insert

    VSX2 (VSX2 Human)
    

    Use

    CRISPR

    Tags

    -p2A-h2b-mRuby3

    Expression

    Mammalian

    Promoter

    Endogenous VSX2 promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. P530_SIX6-p2A-h2b-EGFP

    Plasmid

    #239101

    Purpose

    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of SIX6. This includes the donor casette and a U6 driven guide sequence to be used with Cas9.

    Depositor

    Karl Wahlin

    Article

    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472

    Insert

    SIX6 (SIX6 Human)
    

    Use

    Donor plasmid

    Tags

    p2A-h2b-EGFP

    Expression

    Mammalian

    Promoter

    Endogenous SIX6 promoter

    Available Since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX-N-CTCF-mAID-Halo-donor

    Plasmid

    #139747

    Purpose

    Mouse N-terminal CTCF CRISPR knock-in donor vector with miniAID degron and HaloTag

    Depositor

    Robert Tjian

    Article

    Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

    Insert

    CTCF CRISPR knock-in (Ctcf Mouse)
    

    Use

    CRISPR

    Tags

    mAID-HaloTag

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTR-469 JUNB-tNGFR

    Plasmid

    #186060

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    JUNB (JUNB Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTR-472 FOXO1-tNGFR

    Plasmid

    #186062

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    FOXO1 (FOXO1 Human)
    

    Use

    CRISPR

    Mutation

    WT with tNGFR sequence

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIG-721 CTLA4-GFP T124P

    Plasmid

    #186126

    Purpose

    CTLA4 gene knockin with mutation

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CTLA4 (CTLA4 Human)
    

    Use

    CRISPR

    Mutation

    T124P

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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