We narrowed to 5,822 results for: pCas

1. pCAGG-GST-TEV-mCherry-NAP1 (WT)

   Plasmid

   #198036

   Purpose

   Used for the expression and purification of mCherry NAP1 in a mammalian expression system (SMC Internal No. 2065)

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   NAP1 (AZI2 Human)
   

   Tags

   GST-TEV and mCherry

   Expression

   Mammalian

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCaUra3-CUbo(K48R/K63R)-yeGFP

   Plasmid

   #212774

   Purpose

   C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP in budding yeast with CaUra3 selection

   Depositor

   Helle Ulrich

   Article

   Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

   Type

   Empty backbone

   Use

   Pcr-based tagging

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-GST-ATG13 (363-450)

   Plasmid

   #210849

   Purpose

   Mammalian expression of ATG13 (363-450) mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   ATG13 (ATG13 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-FIP200 (1-640)-R334D-TSF

   Plasmid

   #210841

   Purpose

   Mammalian expression of FIP200 NTD mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Expression

   Mammalian

   Mutation

   R334D

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-FIP200 (1-640)-R326D-TSF

   Plasmid

   #210840

   Purpose

   Mammalian expression of FIP200 NTD mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Expression

   Mammalian

   Mutation

   R326D

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-FIP200 (1-640)-V314D-TSF

   Plasmid

   #210839

   Purpose

   Mammalian expression of FIP200 NTD mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Expression

   Mammalian

   Mutation

   V314D

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-FIP200 (1-640)-V44D-TSF

   Plasmid

   #210843

   Purpose

   Mammalian expression of FIP200 NTD mutant

   Depositor

   James Hurley

   Article

   Chen et al bioRxiv 2023.06.01.543278

   Insert

   FIP200 (RB1CC1 Human)
   

   Expression

   Mammalian

   Mutation

   V44D

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PET-B-HypaR-SpCas9-NLS-6xHis

   Plasmid

   #207388

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HypaR-SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R661A, N692A, M694A, Q695A, H698A, amino acids 10…

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. PET-HypaR-SpCas9-NLS-6xHis

   Plasmid

   #207387

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   HypaR-SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   R661A, N692A, M694A, Q695A, H698A

   Promoter

   T7

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PET-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207382

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    T7

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PET-B-HiFi SpCas9-NLS-6xHis

    Plasmid

    #207378

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HiFi SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    R691A, amino acids 1005-1013 replaced with two gl…

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. PET-Blackjack SpCas9-NLS-6xHis

    Plasmid

    #207375

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Blackjack SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    amino acids 1005-1013 replaced with two glycine

    Promoter

    T7

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-GST-ATG13 (363-460)

    Plasmid

    #203553

    Purpose

    Mammalian expression of GST tagged ATG13 (363-460)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAGGS-SMSr-TEV-Twin-Strep

    Plasmid

    #202530

    Purpose

    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged human Sphingomyelin synthase-relate protein (SMSr) in mammalian cell

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Insert

    SAMD8 (SAMD8 Human)
    

    Tags

    TEV cleavable site and Twin-Strep-tag

    Expression

    Mammalian

    Mutation

    deleted stop codon (TGA)

    Promoter

    CAG and chicken β-actin promoter

    Available Since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-ATG13 (363-517)-MBP

    Plasmid

    #203561

    Purpose

    Mammalian expression of ATG13 (363-517) with MBP tag

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    MBP-10His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-GST-ATG13 (460-517)

    Plasmid

    #203552

    Purpose

    Mammalian expression of GST tagged ATG13 (460-517)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-GST-ATG13 (450-517)

    Plasmid

    #203558

    Purpose

    Mammalian expression of GST tagged ATG13 (450-517)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-TSF-ATG13 (363-517)

    Plasmid

    #203550

    Purpose

    Mammalian expression of TwinStrep-Flag tagged ATG13 (363-517)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    TwinStrep Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-GST-ATG13 (363-398)

    Plasmid

    #203554

    Purpose

    Mammalian expression of GST tagged ATG13 (363-398)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-GST-ATG13 (402-450)

    Plasmid

    #203557

    Purpose

    Mammalian expression of GST tagged ATG13 (402-450)

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    ATG13 (ATG13 Human)
    

    Tags

    GST

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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