We narrowed to 3,756 results for: biorxiv
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Plasmid#217691PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE3 (ADGRE3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-338PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE2 CTF-FLAG
Plasmid#217690PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE2 (ADGRE2 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-517PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
CL7_2xNLS_iGeoCas9(G)_2xNLS_His
Plasmid#222989PurposeBacterial expression of iGeoCas9(G) constructDepositorInsertCL7_2xNLS_iGeoCas9(G)_2xNLS_His
UseCRISPRTagsCL7 and HisExpressionBacterialMutationPromotertacAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICH-nuc-roGFP2-GPXC71S
Plasmid#191760PurposeTi plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758DepositorInsertnuc-roGFP2-GPXC71S
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationGPX has no peroxidatic Cysteine 71, which is repl…PromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
MBP-3C-hNorrin(33-133)-monomeric(C93A/C95A/C131A)-1D4_pAcGP67a
Plasmid#216385PurposeBaculovirus transfer vector to secrete MBP-fused human Norrin (residues 118-218) (monomeric mutant C93A/C95A/C131A)DepositorInsertNorrin (NDP Human)
UseBaculovirusTagsGP64 signal sequence-MBP-3C and Rho1D4ExpressionInsectMutationC93A/C95A/C131APromoterPolyhedrinAvailable sinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-ConVERGD-ArchT(eGFP)-WPRE
Plasmid#218760PurposeProvides AND intersectional (Cre+Flp) expression of ArchT-eGFPDepositorInsertArchT
UseAAVTagseGFPExpressionMutationPromoterhSynAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW (n1)StayGold-T2A-mCherry
Plasmid#205495PurposeExpression of (n1)StayGold-T2A-mCherry in yeastDepositorInsert(n1)StayGold-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
mc2 neo EF1a
Plasmid#206220PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFR-mTq2-antiFRET-P2A-jRCaMP1b
Plasmid#191468PurposeCompare the intensity of the genetically encoded calcium sensor (GECI) jRCaMP1b to mTurquoise2 fluorescent proteinDepositorInsertjRCaMP1b
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF4 CTF-FLAG
Plasmid#217697PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF4 (ADGRF4 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-384PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE1 CTF-FLAG
Plasmid#217689PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE1 (EMR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-584PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
mc2 blast EF1a
Plasmid#206223PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
mc2 mCherry
Plasmid#206219PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
mc2 neo
Plasmid#206214PurposeFor expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceAug. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pICH-chl-roGFP2-GPXC71S
Plasmid#191703PurposeTi plasmid pICH86988 harbouring roGFP2-GPXC71S -a plastid-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191699 and 191701DepositorInsertchl-redox(ro)GFP2-GPXC71S
UseBinary agrobacterium ti plasmidTagsExpressionPlantMutationPeroxidatic Cysteine 71 replaced with SerinePromoterCaMV 35SAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP ratAPOBEC1 L173A/G227A
Plasmid#167169PurposeExpresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.DepositorArticleInsertAPOBEC1 (Apobec1 Rat)
UseTagsExpressionMammalianMutationchanged Leucine 173 to Alanine; changed Glycine 2…PromoterCMV promoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
ER-G-Ca-FLITS-KDEL
Plasmid#191464PurposeGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime changeDepositorInsertG-Ca-FLITS
UseTagsER and KDELExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pminiT-MED6-Halo donor
Plasmid#197866PurposeDonor plasmid used for building MED6-Halo knock-in mESCsDepositorInsertMed6 (Med6 Mouse)
UseMouse TargetingTagsHalotagExpressionMutationPromoterNAAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTS585P-myc
Plasmid#107498PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S585P conferring gain of function phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationS585PPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTG484E-myc
Plasmid#107471PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G484E decreased transport and surface expression relative to wildtypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationG484EPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only