3,636 results

1. pET-PC(1-86)-H6

   Plasmid

   #183780

   Purpose

   Express PC(1-86)-H6 in E. coli. PC(1-86)-H6 was used for GST-CBP pull-down assays

   Depositor

   Peter Harte

   Article

   Tie et al Proc Natl Acad Sci U S A. 2016 Feb 9;113

   Insert

   Polycomb (Pc Fly)
   

   Tags

   6 His Tag at the C-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-SU(Z)12(624-900)-H6

   Plasmid

   #183772

   Purpose

   Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

   Depositor

   Peter Harte

   Article

   Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

   Insert

   SU(Z)12 residues 624-900 (Su(z)12 Fly)
   

   Tags

   His tag at the C-terminus

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAct-GST-H3(K27Q)

   Plasmid

   #182843

   Purpose

   a K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   GST-histone H3 fusion
   

   Tags

   GST

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAct-GST-H3

   Plasmid

   #182842

   Purpose

   A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   GST-histone H3 fusion
   

   Tags

   GST

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-2T-CBP(1603-2678)

   Plasmid

   #183774

   Purpose

   Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

   Depositor

   Peter Harte

   Article

   Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

   Insert

   nej (nej Fly)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-2T-TRX-C751-E3616S

   Plasmid

   #182844

   Purpose

   mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRX (w Fly)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT-FLAG-CBPΔ(N+HAT)

   Plasmid

   #182841

   Purpose

   An EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   CBP (w Fly)
   

   Tags

   FLAG tag

   Expression

   Insect

   Mutation

   dletion of residues 1566-2142 from CBP1476-3222

   Promoter

   MT

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMT-FLAG-CBPΔN

   Plasmid

   #182840

   Purpose

   A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
   

   Tags

   FLAG tag

   Expression

   Insect

   Promoter

   MT

   Available Since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G

   Plasmid

   #146359

   Purpose

   Insect Expression of DmDCP2-PB

   Depositor

   Elisa Izaurralde

   Insert

   DmDCP2-PB (DCP2 Fly)
   

   Expression

   Insect

   Mutation

   Contains an in-frame insertion that adds a Q afte…

   Available Since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1B-EGFP-DmHelz-E958Q_AH

    Plasmid

    #148884

    Purpose

    Insect Expression of DmHelz-E958Q

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    DmHelz-E958Q (CG9425 Fly)
    

    Expression

    Insect

    Mutation

    two silent an two non silent G1374S, R2076G mutat…

    Available Since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1046E=dgRNAs[traB,bTub]

    Plasmid

    #149425

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    U6.3-gRNAs[TraB, bTub]
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6.3

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149427

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[TraB, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pnEA-NpM-DmThor_50-83-GB1_Z

    Plasmid

    #148180

    Purpose

    Bacterial Expression of DmThor_50-83

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor_50-83 (Thor Fly)
    

    Expression

    Bacterial

    Available Since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-EGFP-DmHelz_1213-2103_AH

    Plasmid

    #148883

    Purpose

    Insect Expression of DmHelz_1213-2103

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    DmHelz_1213-2103 (CG9425 Fly)
    

    Expression

    Insect

    Mutation

    two silent an two non silent G1374S, R2076G mutat…

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pnYC-NvM-DmBam_141-442_AG

    Plasmid

    #148833

    Purpose

    Bacterial Expression of DmBam_141-422

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    DmBam_141-422 (bam Fly)
    

    Expression

    Bacterial

    Mutation

    one non silent mutation A239 to S, described as …

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc5.1B-lambdaN-HA-DmPAN3-dsRNAres_Y

    Plasmid

    #148080

    Purpose

    Insect Expression of DmPAN3-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN3-dsRNAres (CG11486 Fly)
    

    Expression

    Insect

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-lambdaN-HA-DmPAN2-F1103A-dsRNAres_Y

    Plasmid

    #148075

    Purpose

    Insect Expression of DmPAN2-F1103A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-F1103A-dsRNAres (PAN2 Fly)
    

    Expression

    Insect

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-MS2-HA-DmRoquin_AD

    Plasmid

    #148531

    Purpose

    Mammalian Expression of DmRoquin

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin (roq Fly)
    

    Expression

    Mammalian

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-lambdaN-HA-DmCaf40-F65AC69Y_X

    Plasmid

    #147993

    Purpose

    Insect Expression of DmCaf40-F65AC69Y

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmCaf40-F65AC69Y (Rcd-1 Fly)
    

    Expression

    Insect

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-E151AF152AN155A-V5His6_AC

    Plasmid

    #148466

    Purpose

    Insect Expression of DmNanos_116-163-E151AF152AN1555A

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmNanos_116-163-E151AF152AN1555A (nos Fly)
    

    Expression

    Insect

    Available Since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge