3,636 results

1. pAct-GST-H3(K27Q)

   Plasmid

   #182843

   Purpose

   a K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   GST-histone H3 fusion
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAct-GST-H3

   Plasmid

   #182842

   Purpose

   A PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   GST-histone H3 fusion
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-2T-CBP(1603-2678)

   Plasmid

   #183774

   Purpose

   Express GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.

   Depositor

   Peter Harte

   Article

   Tie et al Mol Cell Biol. 2012 Jun;32(12):2323-34.

   Insert

   nej (nej Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-2T-TRX-C751-E3616S

   Plasmid

   #182844

   Purpose

   mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRX (w Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT-FLAG-CBPΔ(N+HAT)

   Plasmid

   #182841

   Purpose

   An EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   CBP (w Fly)
   

   Use

   Tags

   FLAG tag

   Expression

   Insect

   Mutation

   dletion of residues 1566-2142 from CBP1476-3222

   Promoter

   MT

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT-FLAG-CBPΔN

   Plasmid

   #182840

   Purpose

   A 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2009 Sep;136(18):3131-41. d

   Insert

   CBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
   

   Use

   Tags

   FLAG tag

   Expression

   Insect

   Mutation

   Promoter

   MT

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G

   Plasmid

   #146359

   Purpose

   Insect Expression of DmDCP2-PB

   Depositor

   Elisa Izaurralde

   Insert

   DmDCP2-PB (DCP2 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Contains an in-frame insertion that adds a Q afte…

   Promoter

   Available since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAc5.1B-EGFP-DmHelz-E958Q_AH

   Plasmid

   #148884

   Purpose

   Insect Expression of DmHelz-E958Q

   Depositor

   Elisa Izaurralde

   Article

   Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

   Insert

   DmHelz-E958Q (CG9425 Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   two silent an two non silent G1374S, R2076G mutat…

   Promoter

   Available since

   April 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. 1046E=dgRNAs[traB,bTub]

   Plasmid

   #149425

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   U6.3-gRNAs[TraB, bTub]
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6.3

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

    Plasmid

    #149427

    Purpose

    The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Inserts

    U6.3-gRNAs[TraB, bTub]
    Hsp70Bb-Cas9-T2A-eGFP-p10
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pnEA-NpM-DmThor_50-83-GB1_Z

    Plasmid

    #148180

    Purpose

    Bacterial Expression of DmThor_50-83

    Depositor

    Elisa Izaurralde

    Article

    Igreja et al Nat Commun. 2014 Sep 2;5:4790. doi: 10.1

    Insert

    DmThor_50-83 (Thor Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAc5.1B-EGFP-DmHelz_1213-2103_AH

    Plasmid

    #148883

    Purpose

    Insect Expression of DmHelz_1213-2103

    Depositor

    Elisa Izaurralde

    Article

    Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

    Insert

    DmHelz_1213-2103 (CG9425 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    two silent an two non silent G1374S, R2076G mutat…

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pnYC-NvM-DmBam_141-442_AG

    Plasmid

    #148833

    Purpose

    Bacterial Expression of DmBam_141-422

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    DmBam_141-422 (bam Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    one non silent mutation A239 to S, described as …

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-lambdaN-HA-DmPAN3-dsRNAres_Y

    Plasmid

    #148080

    Purpose

    Insect Expression of DmPAN3-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN3-dsRNAres (CG11486 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmPAN2-F1103A-dsRNAres_Y

    Plasmid

    #148075

    Purpose

    Insect Expression of DmPAN2-F1103A-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

    Insert

    DmPAN2-F1103A-dsRNAres (PAN2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-MS2-HA-DmRoquin_AD

    Plasmid

    #148531

    Purpose

    Mammalian Expression of DmRoquin

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    DmRoquin (roq Fly)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-lambdaN-HA-DmCaf40-F65AC69Y_X

    Plasmid

    #147993

    Purpose

    Insect Expression of DmCaf40-F65AC69Y

    Depositor

    Elisa Izaurralde

    Article

    Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

    Insert

    DmCaf40-F65AC69Y (Rcd-1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-E151AF152AN155A-V5His6_AC

    Plasmid

    #148466

    Purpose

    Insect Expression of DmNanos_116-163-E151AF152AN1555A

    Depositor

    Elisa Izaurralde

    Article

    Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

    Insert

    DmNanos_116-163-E151AF152AN1555A (nos Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-EGFP-DmGW182_1200-1384_L

    Plasmid

    #146825

    Purpose

    Insect Expression of DmGW182_1200-1384

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

    Insert

    DmGW182_1200-1384 (gw Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-lambdaN-HA-DmRUMP-del233-341_X

    Plasmid

    #147920

    Purpose

    Insect Expression of DmRUMP-del233-341

    Depositor

    Elisa Izaurralde

    Insert

    DmRUMP-del233-341 (rump Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge