Addgene

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pCZ829

Plasmid

#110137

Purpose

Epidermal Mitochondrial-tagged Florescent Calcium Sensor

Depositor

Andrew Chisholm

Article

Xu et al Dev Cell. 2014 Oct 13;31(1):48-60. doi:

Insert

mito-GCaMP5

Use

Tags

mito::GCaMP5

Expression

Worm

Mutation

Promoter

Pcol-19

Available since

May 18, 2018

Availability

Academic Institutions and Nonprofits only

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L4440-daf-16df

Plasmid

#31505

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16d/f specific (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
USP19 (C506A)-FLAG/pRK5

Plasmid

#36307

Depositor

Xiaolu Yang

Article

Mei et al J Biol Chem. 2011 Oct 14;286(41):35380-7

Insert

ubiquitin specific peptidase 19 (USP19 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C506A

Promoter

CMV

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
5.2 kB VEGF-luc

Plasmid

#27987

Depositor

Patricia D'Amore

Article

Loureiro et al Biochem Biophys Res Commun. 2005 Jan 14.

Insert

5.2 kB VEGF

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
L4440-daf-16abf

Plasmid

#31507

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16a/b/f common (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-4

Plasmid

#17995

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-4 (Wnt4 Mouse)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pGCS-C1m(5xMYC)

Plasmid

#85724

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

5xMYC

Expression

Mutation

Promoter

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 3

Plasmid

#30161

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al Neural Dev. 2008 . 3():15.

Insert

APLP2 shRNA 3

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
pRBUBR1

Plasmid

#20198

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 (UBR1 Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pLL3.7_hsa-miR-30c

Plasmid

#25797

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

hsa-miR-30c

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2010

Availability

Academic Institutions and Nonprofits only
pMT p85-GFP

Plasmid

#126660

Purpose

Inducible expression of p85-GFP in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

PI3K SH2 containing subunit

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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RGG-NanoLuc-RGG

Plasmid

#176642

Purpose

NanoLuc enzyme fused to LAF-1 RGG domain

Depositor

Daniel Hammer

Article

Guan et al Biochemistry. 2021 Oct 14. doi: 10.1021/

Insert

RGG-NanoLuc-RGG

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
L4440-daf-16b

Plasmid

#31504

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16b specific (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pAG426Gal-GFP

Plasmid

#38310

Depositor

Susan Lindquist

Article

McLellan et al ACS Chem Biol. 2012 Jun 22.

Insert

EGFP (eGFP )

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 8, 2012

Availability

Academic Institutions and Nonprofits only
pET His10-Src42a

Plasmid

#126674

Purpose

Expression of His10-Src42a for purification from E. coli

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Src42a

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR APLP2 shRNA 1

Plasmid

#30159

Depositor

Tracy Young-Pearse, Dennis Selkoe

Article

Young-Pearse et al Neural Dev. 2008 . 3():15.

Insert

APLP2 shRNA 1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only
L4440-daf-16af

Plasmid

#31506

Depositor

Heidi Tissenbaum

Article

Kwon et al Nature. 2010 Jul 22. 466(7305):498-502.

Insert

daf-16a/f common (daf-16 Nematode)

Use

RNAi

Tags

Expression

Mutation

Promoter

Available since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only
pRubiC

Plasmid

#66700

Purpose

Express mCherry in Newborn neuorns

Depositor

Bryan Luikart

Article

Luikart et al PLoS One. 2011;6(5):e19077. doi: 10.1371

Insert

mCherry

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
pR208IIM1

Plasmid

#45352

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 mutant

Use

Tags

Expression

Yeast

Mutation

P406S, DE317NK

Promoter

ADH

Available since

July 19, 2013

Availability

Academic Institutions and Nonprofits only
V7 pDNR Dual - Donor

Plasmid

#11689

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Donor vector

Tags

Expression

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
V622 pGEX-2TK-LP - Acceptor

Plasmid

#11716

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

May 24, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pR208IM1

Plasmid

#45351

Depositor

Alexander Varshavsky

Article

Xia et al J Biol Chem. 2008 Aug 29. 283(35):24011-

Insert

UBR1 mutant

Use

Tags

Expression

Yeast

Mutation

D176E

Promoter

ADH

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-1

Plasmid

#17991

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-1 (Wnt1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pDUP 4-5

Plasmid

#23023

Depositor

Douglas Black

Article

Modafferi et al Mol Cell Biol. 1997 Nov . 17(11):6537-45

Insert

c-src (Src Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pCMV Ub Lex VP16 HA (#1709)

Plasmid

#14594

Depositor

Matija Peterlin

Article

Kurosu et al Curr Biol. 2004 Jun 22. 14(12):1112-6.

Insert

LexA

Use

Tags

HA, Ubiquitin, and VP16

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
7859 pRS305-pUG72

Plasmid

#10897

Depositor

Susan Lindquist

Article

Cowen et al Science. 2005 Sep 30. 309(5744):2185-9.

Insert

Ura3 (URA3 Budding Yeast)

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 26, 2006

Availability

Academic Institutions and Nonprofits only

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pGL3-PMT-CD41

Plasmid

#25801

Depositor

Judy Lieberman

Article

Navarro et al Blood. 2009 Sep 3. 114(10):2181-92.

Insert

CD41 promoter

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
p6111 pSG5-HPV31-KWK-E8^E2C(HA)

Plasmid

#24742

Depositor

Peter Howley

Article

Powell et al J Virol. 2010 Feb 24. ():.

Insert

HPV31 KWK E8^E2C (HA)

Use

Tags

HA tag in hinge region

Expression

Mammalian

Mutation

residues 5 to 8 from KWK to AEA

Promoter

Available since

June 30, 2010

Availability

Academic Institutions and Nonprofits only
LGmCreER (non-self deleting)

Plasmid

#33342

Depositor

Scott Lowe

Article

Zhao et al Genes Dev. 2010 Jul 1;24(13):1389-402.

Insert

CreERT2

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
V37 pDNR MCS SA - Donor

Plasmid

#11690

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Donor vector

Tags

Expression

Mutation

Promoter

Available since

July 19, 2006

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge