We narrowed to 42,524 results for: cha

1. pETDuet-1 TIM8,13

   Plasmid

   #170282

   Purpose

   Expresses both yeast Tim8 and yeast Tim13 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Sucec et al Sci Adv. 2020 Dec 18;6(51). pii: 6/51/ea

   Insert

   yeast Tim8 and Tim13 (TIM8 Budding Yeast)
   

   Use

   Tags

   TEV-cleavable N-terminal His tag on Tim13 (on bac…

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAG-eYFP-3x-miR708-5p-TS

   Plasmid

   #117381

   Purpose

   An AAV genome containing miRNA target sequence (TS) 708-5p to reduce expression of the fluorescent protein eYFP in neurons

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   EYFP + 3 copies of miR708: CCCAGCTAGATTGTAAGCTCCTT
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CAG-eYFP-3x-miR204-5p-TS

   Plasmid

   #117380

   Purpose

   An AAV genome containing miRNA target sequence (TS) 204-5p to reduce expression of the fluorescent protein eYFP in astrocytes

   Depositor

   Viviana Gradinaru

   Article

   Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

   Insert

   EYFP + 3 copies of miR204 : AGGCATAGGATGACAAAGGGAA
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pECNUS4_MP

   Plasmid

   #228358

   Purpose

   ABE8e-SpCas9-NG Multiplex Acceptor clone for insertion of up to six gRNA cassettes via Golden Gate with BpiI and T4 ligase, blue-white screening

   Depositor

   Eunyoung Chae

   Article

   Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pECNUS2

   Plasmid

   #184885

   Purpose

   ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

   Depositor

   Eunyoung Chae

   Article

   Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pECNUS3

   Plasmid

   #184886

   Purpose

   ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

   Depositor

   Eunyoung Chae

   Article

   Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pECNUS1

   Plasmid

   #184884

   Purpose

   ABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA delivery

   Depositor

   Eunyoung Chae

   Article

   Tan et al Mol Plant Microbe Interact. 2025 Feb;38(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available since

   July 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSP64-mHCN2

   Plasmid

   #53060

   Purpose

   Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes

   Depositor

   Michael Sanguinetti

   Article

   Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7

   Insert

   Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
   

   Use

   Xenopus oocyte expression

   Tags

   Expression

   Mutation

   E55G, R237H, and K283R polymorphisms compared to …

   Promoter

   SP6

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. Syn-ATP

   Plasmid

   #51819

   Purpose

   Optical reporter of presynaptic ATP

   Depositor

   Timothy Ryan

   Article

   Rangaraju et al Cell. 2014 Feb 13;156(4):825-35. doi: 10

   Insert

   Chimeric Synaptophysin-mCherry-Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Within WT luciferase gene, changed Threonine 214 …

   Promoter

   CMV

   Available since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV_OHu19986C_myc tag

    Plasmid

    #183174

    Purpose

    used to make AAV vectors for hsTRPA1 tagged with myc

    Depositor

    Sreekanth Chalasani

    Article

    Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

    Insert

    hsTRPA1 (TRPA1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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