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pBAD_LcgcC-GFP-H6

Plasmid

#245528

Purpose

His-tagged superfolder GFP C-terminally fused to C-terminal LCGC14 split intein fragment

Depositor

Henning Mootz

Article

Humberg et al Angew Chem Int Ed Engl. 2025 Oct 23:e202

Insert

C-terminal Fragment of the LCGC14 PolB2 Split Intein

Tags

Hexahistidine Tag and Superfolder Green Fluoresce…

Expression

Bacterial

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pPB-CrPV-IRES-cytERM-SUNTAG-MS2

Plasmid

#247299

Purpose

Mammalian expression of ER-specific SUNTAG reporter: piggyBac vector expressing cytERM-SUNTAG MS2; 5’ UTR includes cricket paralysis virus IRES, allowing escape from translation initiation regulation.

Depositor

Jennifer Lippincott-Schwartz

Article

Choi et al Nature. 2025 Nov 5. doi: 10.1038/s41586-

Insert

PB-CrPV-IRES-cytERM-SUNTAG-MS2

Expression

Mammalian

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEF1-FRT-HaloTag-TAF1A

Plasmid

#247347

Purpose

Expresses HaloTag-TAF1A

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

TAF1A (TAF1A) (TAF1A Human)

Tags

HaloTag

Expression

Mammalian

Promoter

EF1α

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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huCof WT-mRFP

Plasmid

#245973

Purpose

Neuronal specific expression of cof-mRFP

Depositor

James Bamburg

Article

Flynn et al Dev Neurobiol. 2009 Oct;69(12):761-79. d

Insert

cofilin 1 (cfl1) (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

NSE

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

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GfaABC1D-Lck-mCherry-ibARK

Plasmid

#241241

Purpose

Expression of plasma membrane-tethered ibARK in astrocytes

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

Lck-mCherry-ibARK

Use

AAV

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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hSyn1-mCherry-CAAX

Plasmid

#223674

Purpose

AAV expression of mCherry from hSyn1 promoter

Depositor

Jun Nagai

Article

Dewa et al Nature. 2025 Oct 15. doi: 10.1038/s41586

Insert

mCherry-CAAX

Use

AAV

Promoter

hSyn1

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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CASTOR1-HA (D304A)

Plasmid

#237419

Purpose

Transient expression of C-terminally HA-tagged human CASTOR1 (D304A mutant)

Depositor

David Sabatini

Article

Valenstein et al Nature. 2025 Aug 20. doi: 10.1038/s41586

Insert

CASTOR1 (GATSL3 Human)

Tags

HA

Expression

Mammalian

Mutation

D304A

Available Since

Aug. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-SLC35A1

Plasmid

#239952

Purpose

Encodes gRNA for human SLC35A1

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

SLC35A1

Use

CRISPR

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-MFSD6

Plasmid

#239951

Purpose

Encodes gRNA for human MFSD6

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

CRISPR

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-KS

Plasmid

#238266

Purpose

For overexpression of V5-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-KS

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-ICAM-5-Puro

Plasmid

#239949

Purpose

CRISPR KO of human ICAM-5

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

ICAM-5

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-MFSD6-Puro

Plasmid

#239950

Purpose

CRISPR KO of human MFSD6

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-noKS

Plasmid

#238265

Purpose

For overexpression of V5-nb-noKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-noKS

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry-P2A

Plasmid

#238238

Purpose

For integration of mCherry control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-P2A

Plasmid

#238231

Purpose

For overexpression of mCherry control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG-KS

Plasmid

#238298

Purpose

For RNA injection of Nanog-mNG-KS into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG-KS

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-EGFP-NPM1

Plasmid

#238264

Purpose

For overexpression of V5-EGFP-NPM1

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-EGFP-NPM1

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_mCherry-SURF6

Plasmid

#238252

Purpose

For overexpression of mCherry-SURF6

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry-SURF6

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG

Plasmid

#238296

Purpose

For RNA injection of Nanog-mNG into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_V5-nb-KS-FtoG

Plasmid

#238267

Purpose

For overexpression of V5-nb-KS-FtoG

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

V5-nb-KS-FtoG

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRK5_TagBFP-P2A

Plasmid

#238272

Purpose

For overexpression of TagBFP-control

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

TagBFP

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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eGFP-MYO5C

Plasmid

#239278

Purpose

Expression of N terminal GFP tagged Myo5C

Depositor

David Pellman

Article

Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

Insert

MYO5C (MYO5C Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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p52K-KS-FtoA

Plasmid

#237689

Purpose

For overexpression of 52K-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-KS-FtoA

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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p52K-linkerKS-FtoA

Plasmid

#237693

Purpose

For overexpression of 52K-linkerKS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-linkerKS-FtoA

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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p52K-GFP-KS-FtoA

Plasmid

#237691

Purpose

For overexpression of 52K-GFP-KS-FtoA

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-GFP-KS-FtoA

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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p52K-GFP-linker-KS

Plasmid

#237686

Purpose

For overexpression of 52K-GFP-linker-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-GFP-linker-KS

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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p52K-linkerKS

Plasmid

#237692

Purpose

For overexpression of 52K-linkerKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

52K-linkerKS

Expression

Mammalian

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET22b_msfGFP_stag

Plasmid

#237629

Purpose

For expression of msfGFP_stag in Ecoli

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

stag

Tags

msfGFP

Expression

Bacterial

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pLIB-Strep(II)(N)-PIN1

Plasmid

#231724

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

PIN1 (PIN1 Human)

Tags

Strep(II)-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shScg2-CWB

Plasmid

#223659

Purpose

Expresses hM3D(Gq) and a shRNA targetting Scg2 in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shScg2

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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