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Plasmid#190199PurposeExpresses the small actin binding peptide, LifeAct, with a GFP reporterDepositorInsertLifeAct-eGFP
UseAAVTagsGFPExpressionBacterial and MammalianMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-BioID2-BioID2-HA
Plasmid#176855PurposeExpresses the promiscuous small biotin ligase, BioID2, in neuronsDepositorInsertBioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
GluR2_adRNA(1,20,6)-ADAR2(E488Q)
Plasmid#124621PurposeExpresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)DepositorInsertADAR2(E488Q)
UseAAVTagsExpressionMammalianMutationE488Q hyperactive mutantPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAG-FLEx(FRT)-TC
Plasmid#67827PurposeFlp-dependent expression of TVA-mCherry fusion to mediate modifed rabies virus expression in cellsDepositorInsertTVA-mCherry fusion
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-Aqp4-eGFP
Plasmid#176747PurposeExpresses Aquaporin-4 fused with eGFP at the C-terminus in astrocytesDepositorInsertAqp4-eGFP
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
UPB-colon (UPB4)
Plasmid#180786PurposeUnique Projection Barcode (UPB4)-Colon for Projection-seqDepositorInserthM3Dq ( partial )
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-FLEx(FRT)-G
Plasmid#67828PurposeFlp-dependent expression of rabies glycoprotein to mediate trans-synaptic spread of modifed rabies virus in cellsDepositorInsertrabies glycoprotein
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAM-CAGGS-FLEX-H2B-3XHA-P2A-N2c(G)
Plasmid#73477PurposeEncodes the G protein to enable transsynaptic RAV infectionDepositorInsertH2B-3XHA-P2A-N2c(G)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-Flex-TC66T
Plasmid#48331PurposeCre-dependant TVA receptor-mCherry fusion with a mutation (66T) in TVADepositorInsertTVA receptor mCherry fusion with the 66T mutation
UseAAV and Cre/LoxTagsmCherry fusion to TVA receptorExpressionMammalianMutation66TPromoterCAGAvailable sinceDec. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Glt1-eGFP
Plasmid#176862PurposeExpresses Glt-1 fused with GFP at the C-terminus in astrocytesDepositorInsertGlt1-eGFP
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PE_CO
Plasmid#190335PurposePrime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cellsDepositorInsertPE2 with codon-optimized reverse transcriptase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
px552-sg-gria1-HT-SEP
Plasmid#187653PurposeContains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.DepositorInsertHaloTag-SEP donor
UseAAV and CRISPRTagsN/AExpressionMutationPromoterN/AAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
DIO-EF1a-FLEX-H2B-GFP-P2A-N2c(G)
Plasmid#73476PurposeEncodes the G protein to enable transsynaptic RAV infectionDepositorInsertH2B-GFP-P2A-N2c(G)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
DIO-EF1a-FLEX-H2B-3XHA-P2A-N2c(G)
Plasmid#73478PurposeEncodes the G protein to enable transsynaptic RAV infectionDepositorInsertH2B-3XHA-P2A-N2c(G)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Ezr-eGFP
Plasmid#176856PurposeExpresses Ezrin fused with eGFP at the C-terminus in astrocytesDepositorInsertEzr-GFP
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAM-CAGGS-FLEX-H2B-GFP-P2A-N2c(G)
Plasmid#73475PurposeEncodes the G protein to enable transsynaptic RAV infectionDepositorInsertH2B-GFP-P2A-N2c(G)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX601‐mhCMV‐miniABEmax‐N2‐ E53ogRNA
Plasmid#187065PurposeGp41-1 Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertminiABEmax-Gp41-1
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601‐mhCMV‐ABEmaxNGA‐C2‐ E53ogRNA
Plasmid#187066PurposeGp41-1 Split C-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertGp41-1-ABEmaxNGA
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
AV4-EF1-GFP
Plasmid#124971PurposeControl for AV4-EF1-NMNAT2DepositorInsertGFP
UseAAVTagsExpressionMutationPromoterEF1-alphaAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK- TurboID- NCAN ELS-HA
Plasmid#219809PurposeSecreted HA-tagged TurboID-NCAN-ELS domain for extracellular proximity labelingDepositorInsertGfaABC1D
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK- TurboID- NCAN IL-HA
Plasmid#219808PurposeSecreted HA-tagged TurboID-NCAN-IL domain for extracellular proximity labelingDepositorInsertIgK (Igk Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D -IgK-NCAN ELS-HA
Plasmid#219811PurposeSecreted HA-tagged NCAN-ELS domainDepositorInsertIgK (Igk Mouse)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX552-EF1a-DIO-mCherry_Dicer gRNA
Plasmid#215279PurposeExpresses Dicer gRNA in a Cre dependent mcherry vectorDepositorInsertDicer control gRNA
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-Crym-eGFP
Plasmid#200080PurposeTo study u-crystallin protein interactors, control plasmidDepositorInsertGfaABC1D-Crym-eGFP
UseAAV and Mouse TargetingTagseGFPExpressionMutationPromoterGfaABC1DAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-eGFP-SAPAP3
Plasmid#176853PurposeExpresses SAPAP3 protein fused with eGFP at the N-terminus in neuronsDepositorInserteGFP-SAPAP3
UseAAVTagsGFPExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-eGFP-SAPAP3
Plasmid#176859PurposeExpresses SAPAP3 protein fused with eGFP at the N-terminus in astrocytesDepositorInserteGFP-SAPAP3
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pX601‐mhCMV‐miniABEmax‐N3‐ E53ogRNA
Plasmid#187064PurposeNpu Split N-terminal half of ABEmaxNGA with gRNA targeting mdx4cv nonsense mutationDepositorInsertminiABEmax-Npu
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermhCMVAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NES
Plasmid#124703PurposeExpresses MS2_adRNA targeting the RAB7A locus along with MCP_ADAR1 DD (E1008Q)_NESDepositorInsertMCP_ADAR2 DD (E1008Q)_NES
UseAAVTagsNESExpressionMammalianMutationE1008Q hyperactive mutantPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES
Plasmid#124707PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NESDepositorInsertMCP_ADAR2 DD (E488Q)_NES
UseAAVTagsNESExpressionMammalianMutationE488Q hyperactive mutantPromoterCMVAvailable sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
sUPRa
Plasmid#223242PurposesUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levelsDepositorInsertsUPRa
UseAAVTagsExpressionMutationPromoterCustomAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA
Plasmid#176742PurposeExpresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytesDepositorInsertAqp4-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAP2
Plasmid#73211Purposeexpress Assembly-activating protein (AAP) of adeno-associated virus serotype 2 (AAV2)DepositorInsertAAP2
UseAAVTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-HA-BioID2-SAPAP3
Plasmid#176857PurposeExpresses SAPAP3 protein fused with BioID2 at the N-terminus in neuronsDepositorInsertHA-BioID2-SAPAP3
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
iCAP-ROOT
Plasmid#192262PurposeAAV9 derived Retrograde vector Optimized for Organ Tracing (ROOT) capsidDepositorInsertSynthetic construct for ROOT VP1 gene
UseAAVTagsExpressionMammalianMutationPromoterp41Available sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601‐MHP1‐miniABEmaxNG‐N2‐(GG)
Plasmid#187067PurposeGp41-1 Split N-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutationDepositorInsertminiABEmaxNG-Gp41
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterMHP1Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX601‐MHP1‐ABEmaxC2‐NG‐E53 ogRNA
Plasmid#187068PurposeGp41-1 Split C-terminal half of ABEmaxNGA driven by MHP1 promoter with gRNA targeting mdx4cv nonsense mutationDepositorInsertGp41-ABEmaxC2‐NG
UseAAVTagsExpressionMammalianMutationPromoterMHP1Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
SynTagMA_post
Plasmid#119736PurposeSynaptic Tag for Mapping ActivityDepositorInsertSynTagMA
UseAAVTagsFLAGExpressionMammalianMutationPromoterhSyn-1Available sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
SynTagMA_pre
Plasmid#119738PurposeSynaptic Tag for Mapping ActivityDepositorInsertSynTagMA_pre
UseAAVTagsExpressionMammalianMutationPromoterhsyn1Available sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176741PurposeExpresses membrane bound biotin ligase, BioID2, in astrocytesDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-sgRNA-GFP
Plasmid#200068PurposeFor the knock down of GFP with an astrocyte specific mCherry expressionDepositorInsertU6-sgRNA-GFP
UseAAV, CRISPR, and Mouse TargetingTagsGfaABC1D-mCherryExpressionMutationPromoterU6, GfaABC1DAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
scFLARE1-p2A-eGFP
Plasmid#158699PurposeMammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection markerDepositorInsertscFLARE1-p2A-eGFP
UseAAVTagsFlag, V5. eGFPExpressionMammalianMutationTruncated form of TEV protease (220-242 amino aci…PromoterCMVAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-13xLinker-BioID2-BioID2-HA
Plasmid#176854PurposeExpresses membrane bound biotin ligase, BioID2, in neuronsDepositorInsertLck-13xLinker-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 gfaABC1D-NLS-SaCas9-3xHA-NLS bGH
Plasmid#178960PurposeExpresses saCas9 protein specifically in astrocytesDepositorInsertStaphylococcus aureus Cas9 (SaCas9)
UseAAVTags3x HAExpressionMutationPromotergfaABC1D (astrocyte specific)Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pzac2.1 U6-control sgRNA1, 2, 3_gfaABC1D mcherry SV40
Plasmid#179120PurposeExpresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertcontrol sgRNA
UseAAVTagsExpressionMutationPromoterU6Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pzac2.1 U6-Ezr sgRNA1, 2, 3_gfaABC1D mcherry SV40
Plasmid#179119PurposeExpresses Ezr sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertEzr sgRNA
UseAAVTagsExpressionMutationPromoterU6Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-GAT3-HA-mCherry
Plasmid#117722PurposeExpresses a GABA transporter GAT-3 (Slc6a11)DepositorInsertSlc6a11 (Slc6a11 Mouse)
UseAAVTagsHA and mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pX552-EF1a-DIO-mcherry(with gRNA scaffold)
Plasmid#199581PurposeExpresses mCherry in a Cre-dependent mannerDepositorInsertmCherry
UseAAV, CRISPR, and Cre/LoxTagsExpressionMammalianMutationPromoterEF1a intron A and U6Available sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-Glt1-BioID2-BioID2-HA
Plasmid#176745PurposeExpresses Glt-1 fused with BioID2 at the C-terminus in astrocytesDepositorInsertGlt1-BioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS
Plasmid#124705PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLSDepositorInsertMCP_ADAR2 DD (E488Q)_NLS
UseAAVTagsNLSExpressionMammalianMutationE488Q hyperactive mutantPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
Pzac2.1 U6-Fermt2 sgRNA1, 2, 3_gfaABC1D mcherry SV40
Plasmid#179117PurposeExpresses Fermt2 sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertFermt2 sgRNA
UseAAVTagsExpressionMutationPromoterU6Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only