We narrowed to 5,822 results for: pCas

1. pBW807_pCAG-PV4-loxP-mCherry-BGHpA

   Plasmid

   #87583

   Purpose

   PV4 for 3-input, 2-output Full Adder

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   mCherry
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBW770_pCAG-PV4-loxP-mRuby2-BGHpA

   Plasmid

   #87577

   Purpose

   PV4 for 2-input, 4-output decoder circuit

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   mRuby2
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBW707_pCAG-PV3-FRT-loxP-mCherry

   Plasmid

   #87571

   Purpose

   PV3 for 2-input, 2-output circuit library

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   mCherry
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBW705_pCAG-PV1-FRT-lox2272-STOP

   Plasmid

   #87569

   Purpose

   PV1 for 2-input, 2-output circuit library

   Depositor

   Wilson Wong

   Article

   Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   Neomycin Resistance Gene
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCASCADE-gltA1-gltA2-udhA-zwf

   Plasmid

   #87152

   Purpose

   gltA1-gltA2-udhA-zwf targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   gltA1-gltA2-udhA-zwf guide array
   

   Expression

   Bacterial

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCASCADE-gltA1-gltA2-udhA-GapAP1

   Plasmid

   #87151

   Purpose

   gltA1-gltA2-udhA-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   gltA1-gltA2-udhA-GapAP1 guide array
   

   Expression

   Bacterial

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCASCADE-fabI-gltA1-gltA2-gapAP1

   Plasmid

   #87147

   Purpose

   fabI-gltA1-gltA2-gapAP1 targeting guide RNA E. coli , Low Phosphate Induction

   Depositor

   Michael Lynch

   Insert

   fabI-gltA1-gltA2-gapAP1 guide array
   

   Expression

   Bacterial

   Promoter

   E . coli ugpB gene promoter, low phosphate induct…

   Available Since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pzk408-pmax-3xflag-dpspCas13b(AAAA)-NES

   Plasmid

   #196828

   Purpose

   Express dpdpCas13b with AAAA mutation

   Depositor

   Albert Cheng

   Article

   Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

   Insert

   dpspCas13b(AAAA)
   

   Tags

   3XFLAG and NES

   Expression

   Mammalian

   Mutation

   K367A, D369A and K370A

   Available Since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCAG-CBE4max-SpRY-P2A-EGFP (RTW5133)

   Plasmid

   #139999

   Purpose

   CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized CBE4max SpCas9 variant named SpRY with P2A-EGFP
   

   Use

   CRISPR

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpRY=A61R/L1111R/D1135L/S1136W/G121…

   Promoter

   CAG

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid

    #139998

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CAG

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid

    #185910

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available Since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid

    #185914

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV-T7-ABE8.20m-nSpCas9-NG-P2A-EGFP (KAC1164)

    Plasmid

    #185919

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Insert

    human codon optimized ABE8.20m-nSpCas9-NG-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…

    Promoter

    CMV and T7

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Ef1a-pspCas13b-NES-3xFLAG-T2A-BFP

    Plasmid

    #173029

    Purpose

    For expression of cytoplasmic pspCas13b protein tagged with 3xHA-T2A-BFP for gene silencing in mamallian cells.

    Depositor

    Mohamed Fareh

    Article

    Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

    Insert

    pspCas13b
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG, BFP, HIV NES, and T2A

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS DRR mCherry Donor EF1a BFP

    Plasmid

    #98896

    Purpose

    Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    Neomycin-T2A-mCherry-GFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mCherry-pCAG-donor_opt(R2Tg)-GFP(intron)

    Plasmid

    #223249

    Purpose

    Mammalian expression of the optimized R2Tg RNA donor

    Depositor

    Wei Li

    Article

    Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

    Insert

    R2Tg donor_opt
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKS021 - pCAGGS-3XFLAG-mKate2-SA1-bpA

    Plasmid

    #156440

    Purpose

    For transient expression of mouse SA1 (= STAG1 or SCC3A) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA1 (Stag1 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

    Plasmid

    #156445

    Purpose

    For transient expression of mouse RAD21 (SCC1) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse RAD21 (Rad21 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKS037 - pCAGGS-3XFLAG-Smc3-mKate2-bpA

    Plasmid

    #156447

    Purpose

    For transient expression of mouse SMC3 tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SMC3 (Smc3 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

    Plasmid

    #156441

    Purpose

    For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse SA2 (Stag2 Mouse)
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge