Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 5,049 results for: golden gate

Clear keywords
Showing: 221 - 240 of 5049 results

  1. pICSL50047

    Plasmid
    #245603
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag N-terminal portion of split Luciferase
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245603-plasmid-map-sequence-id-495518

    Enlarge

  2. pICSL50048

    Plasmid
    #245604
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag C-terminal portion of split Luciferase
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245604-plasmid-map-sequence-id-495519

    Enlarge

  3. pICSL50034

    Plasmid
    #245592
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag eGFP
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation, also c…
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245592-plasmid-map-sequence-id-495512

    Enlarge

  4. pICSL50005

    Plasmid
    #245578
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag YFP tag (yellow variant of GFP)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245578-plasmid-map-sequence-id-495501

    Enlarge

  5. pICSL60025

    Plasmid
    #245673
    Purpose
    Level 0 Golden Gate part Rb7MAR (D144-437 variant) terminator, GCTT - CGCT
    Depositor
    Mark Youles
    Insert
    Rb7MAR (D144-437 variant) terminator
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245673-plasmid-map-sequence-id-495495

    Enlarge

  6. pICSL30063

    Plasmid
    #245566
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving HF tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245566-plasmid-map-sequence-id-495490

    Enlarge

  7. pICSL30064

    Plasmid
    #245567
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving STREPII tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245567-plasmid-map-sequence-id-495491

    Enlarge

  8. pICSL30065

    Plasmid
    #245568
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving SPY tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245568-plasmid-map-sequence-id-495492

    Enlarge

  9. pICSL30062

    Plasmid
    #245565
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving SUMO tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245565-plasmid-map-sequence-id-495489

    Enlarge

  10. pICSL13020

    Plasmid
    #245664
    Purpose
    Level 0 Golden Gate part EC1.2-EN-EC1.1 Enhancer Fusion promoter, GGAG - CCAT
    Depositor
    Mark Youles
    Insert
    EC1.2-EN-EC1.1 Enhancer Fusion promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245664-plasmid-map-sequence-id-495486

    Enlarge

  11. pICSL12080

    Plasmid
    #245650
    Purpose
    Level 0 Golden Gate part EC1.2-EN-EC1.1 Enhancer Fusion promoter, GGAG - AATG
    Depositor
    Tom Lawrenson, Mark Youles
    Insert
    EC1.2-EN-EC1.1 Enhancer Fusion promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245650-plasmid-map-sequence-id-495475

    Enlarge

  12. pICSL30001

    Plasmid
    #245529
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag SplitYFP tag- N terminus of YFP
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245529-plasmid-map-sequence-id-495461

    Enlarge

  13. pICSL12007

    Plasmid
    #245628
    Purpose
    Level 0 Golden Gate part pAt3: Cysteine Synthase (At3g61440) promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    pAt3: Cysteine Synthase (At3g61440) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245628-plasmid-map-sequence-id-495458

    Enlarge

  14. pOPARA2

    Plasmid
    #200417
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (spectinomycin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200417-plasmid-map-sequence-id-394144

    Enlarge

  15. pOPARA1

    Plasmid
    #200416
    Purpose
    Golden Gate assembly of expression constructs containing pBAD promoter (carbenicillin resistance)
    Depositor
    Kamil Witek
    Article
    Lesley et al bioRxiv
    Type
    Empty backbone
    Expression
    Bacterial
    Promoter
    pBAD
    Available Since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    200416-plasmid-map-sequence-id-394143

    Enlarge

  16. pTALE64

    Plasmid
    #35189
    Purpose
    Golden Gate Compatible TALE Transcription Factor using VP64 Domain
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Transcription Factor
    Use
    TALEN
    Expression
    Mammalian
    Mutation
    +63 C Terminus, VP64 Domain
    Promoter
    CMV
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    35189-plasmid-map-sequence-id-68899

    Enlarge

  17. pICSL41258_CP

    Plasmid
    #245691
    Purpose
    Level 0 Golden Gate Signal Peptide acceptor with AmilCP negative selection cassette AATG - AGGT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245691-plasmid-map-sequence-id-495769

    Enlarge

  18. pICSL61003

    Plasmid
    #245680
    Purpose
    Level 0 Golden Gate part 3' Extensin-Full length-IEU (TerP1) terminator P1, GCTT - TAGA
    Depositor
    Mark Youles
    Insert
    3' Extensin-Full length-IEU (TerP1) terminator P1
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245680-plasmid-map-sequence-id-495766

    Enlarge

  19. pICSL12049

    Plasmid
    #245646
    Purpose
    Level 0 Golden Gate part Double (enhancer)-34S FMV (Figwort Mosaic Virus). promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    Double (enhancer)-34S FMV (Figwort Mosaic Virus). promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245646-plasmid-map-sequence-id-495542

    Enlarge

  20. pICSL41276_CP

    Plasmid
    #245693
    Purpose
    Level 0 Golden Gate Terminator acceptor with AmilCP negative selection cassette GCTT - CGCT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245693-plasmid-map-sequence-id-495522

    Enlarge

Showing: 221 - 240 of 5049 results