Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,722 results for: golden gate

Clear keywords
Showing: 221 - 240 of 4722 results

  1. pICSL30061

    Plasmid
    #245564
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving MBP tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245564-plasmid-map-sequence-id-495560

    Enlarge

  2. pICSL12048

    Plasmid
    #245645
    Purpose
    Level 0 Golden Gate part Single-34S FMV (Figwort Mosaic Virus). promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    Single-34S FMV (Figwort Mosaic Virus). promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245645-plasmid-map-sequence-id-495541

    Enlarge

  3. pICSL12028

    Plasmid
    #245635
    Purpose
    Level 0 Golden Gate part pAt2: Rib Prot16 (At4g34620) promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    pAt2: Rib Prot16 (At4g34620) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245635-plasmid-map-sequence-id-495533

    Enlarge

  4. pICSL50058

    Plasmid
    #245613
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag TurboID+ V5. With GUS Intron and GGGS(x3) linker
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245613-plasmid-map-sequence-id-495523

    Enlarge

  5. pICSL50047

    Plasmid
    #245603
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag N-terminal portion of split Luciferase
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245603-plasmid-map-sequence-id-495518

    Enlarge

  6. pICSL50048

    Plasmid
    #245604
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag C-terminal portion of split Luciferase
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245604-plasmid-map-sequence-id-495519

    Enlarge

  7. pICSL50034

    Plasmid
    #245592
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag eGFP
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation, also c…
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245592-plasmid-map-sequence-id-495512

    Enlarge

  8. pICSL01008_CP

    Plasmid
    #245690
    Purpose
    Level 0 Golden Gate Promoter + 5' UTR acceptor with AmilCP negative selection cassette GGAG - CCAT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245690-plasmid-map-sequence-id-495508

    Enlarge

  9. pICSL01002_CP

    Plasmid
    #245687
    Purpose
    Level 0 Golden Gate N-terminal tag acceptor with AmilCP negative selection cassette CCAT - AATG
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245687-plasmid-map-sequence-id-495506

    Enlarge

  10. pICSL50005

    Plasmid
    #245578
    Purpose
    Level 0 Golden Gate part C-Terminal Tag with overhangs TTCG - GCTT
    Depositor
    Mark Youles
    Insert
    C-Terminal tag YFP tag (yellow variant of GFP)
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245578-plasmid-map-sequence-id-495501

    Enlarge

  11. pICSL60025

    Plasmid
    #245673
    Purpose
    Level 0 Golden Gate part Rb7MAR (D144-437 variant) terminator, GCTT - CGCT
    Depositor
    Mark Youles
    Insert
    Rb7MAR (D144-437 variant) terminator
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245673-plasmid-map-sequence-id-495495

    Enlarge

  12. pICSL30063

    Plasmid
    #245566
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving HF tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245566-plasmid-map-sequence-id-495490

    Enlarge

  13. pICSL30064

    Plasmid
    #245567
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving STREPII tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245567-plasmid-map-sequence-id-495491

    Enlarge

  14. pICSL30065

    Plasmid
    #245568
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving SPY tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245568-plasmid-map-sequence-id-495492

    Enlarge

  15. pICSL30062

    Plasmid
    #245565
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag Self-Cleaving SUMO tag compatible with ProteinSelect purification system
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245565-plasmid-map-sequence-id-495489

    Enlarge

  16. pICSL13020

    Plasmid
    #245664
    Purpose
    Level 0 Golden Gate part EC1.2-EN-EC1.1 Enhancer Fusion promoter, GGAG - CCAT
    Depositor
    Mark Youles
    Insert
    EC1.2-EN-EC1.1 Enhancer Fusion promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245664-plasmid-map-sequence-id-495486

    Enlarge

  17. pICSL12080

    Plasmid
    #245650
    Purpose
    Level 0 Golden Gate part EC1.2-EN-EC1.1 Enhancer Fusion promoter, GGAG - AATG
    Depositor
    Tom Lawrenson, Mark Youles
    Insert
    EC1.2-EN-EC1.1 Enhancer Fusion promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245650-plasmid-map-sequence-id-495475

    Enlarge

  18. pICSL30001

    Plasmid
    #245529
    Purpose
    Level 0 Golden Gate part N-Terminal Tag with overhangs CCAT - AATG
    Depositor
    Mark Youles
    Insert
    N-Terminal tag SplitYFP tag- N terminus of YFP
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245529-plasmid-map-sequence-id-495461

    Enlarge

  19. pICSL12007

    Plasmid
    #245628
    Purpose
    Level 0 Golden Gate part pAt3: Cysteine Synthase (At3g61440) promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    pAt3: Cysteine Synthase (At3g61440) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available Since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245628-plasmid-map-sequence-id-495458

    Enlarge

  20. pOGG012

    Plasmid
    #113986
    Purpose
    Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors
    Depositor
    Philip Poole
    Article
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Partition genes (parABCDE)
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available Since
    Jan. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    113986-plasmid-map-sequence-id-223365

    Enlarge

Showing: 221 - 240 of 4722 results