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AiP15820 - pAAV-AiE0873m_3xC2-minBG-RiboL1-jGCaMP8s-WPRE3-BGHpA (Alias: CN5820)

Plasmid

#224205

Purpose

AiE0873m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal cholinergic neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

RiboL1-jGCaMP8s

Use

AAV

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pKlab1158-HBEGF-2a-3xFLAG-BRCA1(full-length)-R1699Q-2a-mRuby3-HBBIVS2-WPRE

Plasmid

#248998

Purpose

This plasmid expresses the BRCA1 sequence with R1699Q mutation, the construct includes a HBBIVS2 intron sequence at the 3' end of the mRuby sequence to improve the stable integration into cell lines

Depositor

Georgios Karras

Article

Gracia et al Mol Cell. 2025 Nov 19:S1097-2765(25)0086

Insert

BRCA1 (BRCA1 Human)

Use

CRISPR

Tags

3x-FLAG-V5

Mutation

R1699Q

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST (E44A L47A D49A A50K Q51A; deltaWIPI2)

Plasmid

#223777

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

Insert

BNIP3 soluble part (BNIP3 Human)

Tags

Thrombin cleavage-GST

Expression

Insect

Mutation

E44A L47A D49A A50K Q51A; disrupts WIPI2 binding

Available Since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAT3158-{PB3-Puro-EGFP_7xU6-sgRNA-5xPBSa [MED1-Rank3_COLO320 DM_ AT_sg-290-296])

Plasmid

#251115

Purpose

Expression multiple sgRNAs

Depositor

Chia-Lin Wei

Article

Taghbalout et al Cancer Cell. 2025 Dec 8;43(12):2191-2205

Insert

sgRNAs

Expression

Mammalian

Mutation

N/A

Available Since

March 24, 2026

Availability

Academic Institutions and Nonprofits only

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pJET1.2-OsMac3

Plasmid

#218542

Purpose

Vector pJET1.2 contains the 158-bp 5′-UTR fragment (from −158 to −1 bp before the ATG) of OsMac3 from Japonica rice genomic DNA.

Depositor

Kirill Demchenko

Article

Kiryushkin et al Int J Mol Sci. 2024 Feb 6;25(4):1975. do

Insert

158-bp 5′-UTR fragment (from −158 to −1 bp before the ATG) of OsMac3 from Japonica rice genomic DNA.

Use

Synthetic Biology

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pPD128 AND Gate, 3 repeats

Plasmid

#138937

Purpose

EYFP reporter vector for the Logic 37 AND 158 NOT 43 3 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 158 NOT 43 3 repeats

Use

Synthetic Biology

Expression

Mammalian

Promoter

Logic 37 AND 158 NOT 43 3 repeats

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD187 Tri-AND Gate, 2 repeats

Plasmid

#138940

Purpose

EYFP reporter vector for the Logic 37 AND 43 AND 158 2 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 43 AND 158 2 repeats

Use

Synthetic Biology

Expression

Mammalian

Promoter

Logic 37 AND 43 AND 158 2 repeats

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD186 Tri-AND Gate, 1 repeat

Plasmid

#138939

Purpose

EYFP reporter vector for the Logic 37 AND 43 AND 158 1 repeat promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 43 AND 158 1 repeat

Use

Synthetic Biology

Expression

Mammalian

Promoter

Logic 37 AND 43 AND 158 1 repeat

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD129 AND Gate, 4 repeats

Plasmid

#138938

Purpose

EYFP reporter vector for the Logic 37 AND 158 NOT 43 4 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 158 NOT 43 4 repeats

Use

Synthetic Biology

Expression

Mammalian

Promoter

Logic 37 AND 158 NOT 43 4 repeats

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPD127 AND Gate, 2 repeats

Plasmid

#138936

Purpose

EYFP reporter vector for the Logic 37 AND 158 NOT 43 2 repeats promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

Logic 37 AND 158 NOT 43 2 repeats

Use

Synthetic Biology

Expression

Mammalian

Promoter

Logic 37 AND 158 NOT 43 2 repeats

Available Since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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