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Showing: 6001 - 6050 of 18950 results

  13. pOCC154

    Plasmid
    #118902
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
    Use
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118902-plasmid-map-sequence-id-232325

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  16. Puro-iDEST

    Plasmid
    #75336
    Purpose
    GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression
    Depositor
    Danwei Huangfu
    Article
    Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-
    Insert
    TRE-DEST
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE-tight
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    75336-plasmid-map-sequence-id-149881

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  19. pOCC153

    Plasmid
    #118901
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    TwinStrep, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118901-plasmid-map-sequence-id-232312

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  22. PB-hEF1A-MG-U6-sgRNA

    Plasmid
    #175505
    Purpose
    All-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAs
    Depositor
    George Church
    Article
    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a, U6
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175505-plasmid-map-sequence-id-345098

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  36. pENeGFP PCNAL2 pc653

    Plasmid
    #167564
    Purpose
    The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.
    Depositor
    Cristina Cardoso
    Article
    Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.
    Insert
    Proliferating Cell Nuclear Antigen (PCNA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    167564-plasmid-map-sequence-id-388295

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  42. pXPR_212

    Plasmid
    #133457
    Purpose
    U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance
    Depositor
    John Doench
    Article
    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1
    Insert
    control guide
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    133457-plasmid-map-sequence-id-261407

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Showing: 6001 - 6050 of 18950 results