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pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500

Plasmid

#45779

Depositor

Richard Murray, Vincent Noireaux

Insert

sigma factor 28

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

Available Since

July 19, 2013

Availability

Academic Institutions and Nonprofits only

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pBFC0993

Plasmid

#231993

Purpose

CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with negative control (RFP-targeting) spacer

Depositor

Brady Cress

Article

Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

Inserts

dRfxCas13d
crRNA with negative control (RFP-targeting) spacer

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

J23119 and pTet

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pVHC_PGKneoLox2DTA.2

Plasmid

#112622

Purpose

H2BVenus_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Venus (H2BC3 Aequorea victoria, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

Venus

Promoter

No Promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pPLP1.5

Plasmid

#22614

Depositor

Lynn Hudson

Article

Puckett et al J Neurosci Res. 1987 . 18(4):511-8.

Insert

human Proteolipid Protein cDNA (PLP1 Human)

Use

E. coli phagemid vector

Available Since

Nov. 24, 2009

Availability

Academic Institutions and Nonprofits only
pCAM17

Plasmid

#86473

Purpose

Expresses p12 and p9

Depositor

Pamela Silver

Article

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p12, p9

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTW195b63

Plasmid

#136931

Purpose

Directed evolution of SpCas11

Depositor

David Liu

Article

Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

Insert

gIII(N)-npuN

Use

CRISPR

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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LacI-pLacI-pIV pm

Plasmid

#218101

Purpose

Phagemid that expresses LacI, and pIV under the Lac promoter

Depositor

Kevin Esvelt

Article

Gretton et al bioRxiv 2024.03.20.585782

Insert

pIV

Expression

Bacterial

Promoter

Lac promoter

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pBS596 tet-hCMV-GFPcre

Plasmid

#11961

Depositor

Brian Sauer

Article

Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

Insert

GFP-cre (cre bacteriophage P1)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 28, 2006

Availability

Academic Institutions and Nonprofits only
fabp10a:CreERT2; cryaa:mCerulean

Plasmid

#230044

Purpose

Inducible Cre expression in Zebrafish hepatocytes

Depositor

Sumeet Pal Singh

Article

Eski et al Nat Commun. 2025 Jun 6;16(1):5260. doi:

Insert

CreERT2

Use

Cre/Lox; Zebrafish expression

Promoter

fabp10a

Available Since

Feb. 11, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCAM105

Plasmid

#86479

Purpose

Expresses p9-FLAG-FMO

Depositor

Pamela Silver

Article

Myhrvold et al ACS Synth Biol. 2016 Aug 3.

Insert

p9-FLAG-FMO

Use

Synthetic Biology

Tags

FLAG and FMO

Expression

Bacterial

Promoter

pTet

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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Pr1-T7RNAP

Plasmid

#67738

Purpose

OR2-OR1-Pr1 promoter expressing T7 RNAP

Depositor

Richard Murray

Article

Siegal-Gaskins et al ACS Synth Biol. 2014 Jun 20;3(6):416-25.

Insert

UTR1-T7RNAP-T500

Use

Synthetic Biology

Expression

Bacterial

Promoter

OR2-OR1-Pr1

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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p3E p2a-tomato

Plasmid

#135211

Purpose

Red fluorescent tag

Depositor

David Tobin

Article

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

tdTomato

Use

Synthetic Biology

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pQE-WT-KIF-R5-IRH

Plasmid

#63629

Purpose

K1F-R5-IRH RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

KIF-R5-IRH RNAP

Mutation

L749I, Q754S, R756N, I761V, H772R, and Q786H

Promoter

T5/Lac

Available Since

June 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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dCas12j2 KRAB

Plasmid

#188508

Purpose

Expresses FLAG tagged dCas12j2 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j2

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D394A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pQE-WT-KIF-R5-KIKR

Plasmid

#63624

Purpose

KIF-R5-KIKR RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

KIF-R5-KIKR RNAP

Mutation

Q744K, L749I, M750K, Q754S, R756N, I761V, H772R

Promoter

T5/Lac

Available Since

April 15, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-hA3D-BE3

Plasmid

#113413

Purpose

Expresses hA3D-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3D-BE3 (APOBEC3D Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-hA3F-BE3

Plasmid

#113414

Purpose

Expresses hA3F-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hA3F-BE3 (APOBEC3F Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pGem-PgpdA-Cre-TtrpC

Plasmid

#168784

Purpose

Helper vector for the transient expression of Cre recombinase in filamentous fungi. Not replicative in fungi.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2022 Feb 16. doi: 10.102

Insert

Cre

Use

Cre/Lox and Synthetic Biology; Expression in fila…

Promoter

PgpdA

Available Since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pQE-WT-N4-R5-YRNRV

Plasmid

#63625

Purpose

N4-R5-YRNRV RNAP mutant

Depositor

Andrew Ellington

Article

Meyer et al ACS Synth Biol. 2014 Oct 7.

Insert

N4-R5-YRNRV RNAP

Mutation

N671Y, L747I, N748D, L749C, M750V, F751I, Q754T, …

Promoter

T5/Lac

Available Since

June 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-hAID-BE3

Plasmid

#113417

Purpose

Expresses hAID-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

hAID-BE3 (AICDA Human, S. pyogenes and Bacteriophage PBS2)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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