We narrowed to 2,459 results for: GEM

1. pFBD-Akt1(K14A)

   Plasmid

   #86562

   Purpose

   Expresses human full-length Akt1(K14A) mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   K14A mutation in the PH domain

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLG1-puro-sgATL3-2

   Plasmid

   #109010

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL3-sgRNA #2 (ATL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-X1-Neo-HA-UFL1-RAMP4

   Plasmid

   #139863

   Purpose

   Lentiviral expression of fusion of human UFL1 (full length) with RAMP4

   Depositor

   Jacob Corn

   Article

   Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

   Insert

   UFL1-RAMP4 (UFL1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   recombinant fusion of UFL1 (full length) with RAM…

   Promoter

   Available since

   March 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCEP4-HA-Flag-natT1R2 ECD

   Plasmid

   #113954

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Use

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   residues 22-568

   Promoter

   cmv

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCit-Snap25b D179K A199R

   Plasmid

   #53259

   Purpose

   SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   SNAP-25B (Snap25 Rat)
   

   Use

   Tags

   4aa-linker and mCitrine

   Expression

   Mammalian

   Mutation

   D179K and A199R

   Promoter

   CMV

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLG1-puro-sgATL3-5

   Plasmid

   #109011

   Purpose

   CRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   ATL3-sgRNA #5 (ATL3 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mU6 promoter

   Available since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRTCLCrVA009

   Plasmid

   #22842

   Depositor

   Niki Robertson

   Article

   Tuttle et al Plant Physiol. 2008 Sep . 148(1):41-50.

   Insert

   A DNA containing CHLI fragment
   

   Use

   Cotton vigs vector

   Tags

   Expression

   Bacterial

   Mutation

   Cotton Leaf Crumple Virus Coat Protein (AR1 gene)…

   Promoter

   Available since

   June 14, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTet02

   Plasmid

   #78284

   Purpose

   mRFP1 expressed behind an ATc-inducible construct

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   Tet inducible mRFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFBD-Akt1(D323A/D325A)

   Plasmid

   #86592

   Purpose

   Expresses human full-length Akt1(D323A/D325A) mutant

   Depositor

   Thomas Leonard, Ivan Yudushkin

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   D323A/D325A transforming mutations

   Promoter

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. MIGR1_FLAG_TAL1-long_dt tomato

    Plasmid

    #210639

    Purpose

    Overexpression of TAL1-long

    Depositor

    Maayan Salton

    Article

    Sharma et al PLoS Biol. 2023 Jun 28;21(6):e3002175. d

    Insert

    TAL1-long (TAL1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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