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pHR_PGK_SNIPR_hsNotch1 LNR, Notch2 JMD

Plasmid

#188374

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch1-ECD, a Notch1-TMD, a Notch2-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch1-ECD_Notch1-TMD_Notch2-JMD_Gal4VP64

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD-E12

Plasmid

#25990

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Expression

Mutation

Promoter

Available since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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Cathepsin S

Plasmid

#84694

Purpose

for expression of Cathepsin S in Leishmania tarantolae

Depositor

Hans Brandstetter

Article

Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

Insert

cathepsin S (CTSS Human)

Use

Expression in leishmania tarantolae

Tags

his-tag

Expression

Mutation

Promoter

unknown

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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768 pME mApple-CAAX

Plasmid

#195961

Purpose

Gateway pME fluorophore

Depositor

Christian Mosimann, Kristen Kwan

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

mApple-CAAX (membrane)

Use

Other

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCSF107mT-GATEWAY-3'-3HA

Plasmid

#67616

Purpose

A pCS2 based vector to generate a 3'-3HA tag using GATEWAY cloning technology

Depositor

Todd Stukenberg

Article

Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

Type

Empty backbone

Use

In vitro transcription or translation off of an s…

Tags

three inframe 3' HA tags

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
GADD45 WT-luc

Plasmid

#8356

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

GADD45 promoter WT (GADD45A Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pBTK402

Plasmid

#110598

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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Myristoylated optoDroplet

Plasmid

#111507

Purpose

For membrane localized optoDroplets

Depositor

Jared Toettcher

Article

Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

Insert

Cry2 (CRY2 Mustard Weed)

Use

Lentiviral

Tags

FUS, Fusion Red, and Myristoylation Sequence (MYR…

Expression

Mutation

Promoter

SFFV

Available since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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H_NES 41kDa

Plasmid

#201346

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

HIV_NES-EGFP-2PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-LAP1_2-mCherry

Plasmid

#159526

Purpose

Expresses mCherry from LAP1_2 promoter

Depositor

Esteban Engel

Article

Maturana et al Mol Ther Methods Clin Dev. 2020 Apr 14;1

Insert

mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

LAP1_2

Available since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBTK501

Plasmid

#110602

Purpose

BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

GFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pQCXIP TBRII

Plasmid

#19147

Depositor

Joan Massague

Article

Xi et al J Biol Chem. 2008 Jan 11. 283(2):1146-55

Insert

Transforming growth factor, beta receptor II (TGFBR2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
pSL2621 (pCas8_Vch)

Plasmid

#200893

Purpose

Expresses human codon-optimized V. cholerae Cas8 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas8

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSL2623 (pCas6_Vch)

Plasmid

#200895

Purpose

Expresses human codon-optimized V. cholerae Cas6 from a CMV promoter.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCas6

Use

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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dSaCas9-VPR

Plasmid

#205251

Purpose

In vitro transcription plasmid for production of dSaCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #68495

Depositor

Rasmus Bak

Article

Jensen et al Genome Res. 2021 Nov;31(11):2120-2130. d

Insert

dSaCas9-VPR

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pBTK570

Plasmid

#110615

Purpose

BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

E2-Crimson

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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M_NLS 41kDa

Plasmid

#201341

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

(NLS)SV40A5-EGFP-2PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLR5-CAG-NLS-SNAP

Plasmid

#174879

Purpose

PiggyBac transposon vector for expression of NLS-SNAP

Depositor

Hiroshi Ochiai

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

NLS-SNAP

Use

Piggybac vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHS0663 pET45b(+) KraIscB-1 TwinStrep

Plasmid

#176536

Purpose

Bacterial expression of KraIscB-1-TwinStrep

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

KraIscB-1

Use

Tags

TwinStrep

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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L_NES 41kDa

Plasmid

#201347

Purpose

Nucleocytoplasmic transport probe

Depositor

Pere Roca-Cusachs

Article

Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

Insert

Adeno_NES-EGFP-2PrA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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