Addgene

We narrowed to 8,068 results for: gnal

Showing: 3631 - 3660 of 8068 results

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pMA6-TAF4-3xFLAG

Plasmid

#31901

Depositor

Robert Tjian

Article

Wright et al Proc Natl Acad Sci U S A. 2009 Jan 6;106

Insert

TAF4 (Taf4 Fly)

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

Actin 5C

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only
pQCXIH-Flag Smad3 AAGY

Plasmid

#27027

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

P181A P182A

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
XE156 delta LZ-XDpr-CS2+

Plasmid

#16766

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

delta LZ-XDpr (dact1-b Frog)

Use

Xenopus expression

Tags

Expression

Mutation

deletion of first 129 amino acids, including the …

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
XE155 XDpr-delta PDZB-CS2+

Plasmid

#16767

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr-delta PDZB (dact1-b Frog)

Use

Xenopus expression

Tags

Expression

Mutation

last four amino acids deleted

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
XE154 XDpr-pt mutant-CS2+

Plasmid

#16768

Depositor

Randall Moon

Article

Cheyette et al Dev Cell. 2002 Apr. 2(4):449-61.

Insert

XDpr-pt mutant (dact1-b Frog)

Use

Xenopus expression

Tags

Expression

Mutation

last four amino acids mutated to MNTV instead of …

Promoter

Available since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pFastBac(His10-Lckg2a,Y394F)

Plasmid

#177893

Purpose

Baculo expression of His10 tag fused to human LckG2A

Depositor

Ron Vale

Article

Hui et al Nat Struct Mol Biol. 2014 Feb;21(2):133-

Insert

Lck (LCK Human)

Use

Tags

10xHis-TEV

Expression

Insect

Mutation

G2A,Y394F

Promoter

Polyhedrin

Available since

Availability

Academic Institutions and Nonprofits only

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pCAG-TetON-3G

Plasmid

#96963

Purpose

Mammalian expression of Clontech rtTA driven by human beta-actin promoter

Depositor

Elena Cattaneo

Article

Faedo et al Proc Natl Acad Sci U S A. 2017 Feb 14;11

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2017

Availability

Academic Institutions and Nonprofits only

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GfaABC1D BARK p2A mCherry

Plasmid

#117691

Purpose

GfaABC1D-iBARK-p2A-mCherry: Viral expression vector for astrocyte Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs peptide

Use

AAV

Tags

HA / FLAG

Expression

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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CAG FLEX BARK p2A mCherry

Plasmid

#117693

Purpose

CAG-FLEx-iBARK-p2A-mCherry: Viral expression vector for Cre-dependent Gaq silencing

Depositor

Baljit Khakh

Article

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs peptide

Use

Cre/Lox

Tags

HA / FLAG

Expression

Mutation

Promoter

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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Matrix-roGFP

Plasmid

#49437

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

Matrix-roGFP2

Use

Adenoviral

Tags

Cytochrome c oxidase subunit IV mitochondrial tar…

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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GPD-roGFP

Plasmid

#49436

Purpose

Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial intermembrane space of mammalian cells

Depositor

Paul Schumacker

Article

Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

Insert

GPD-roGFP2

Use

Adenoviral

Tags

Glycerol phosphate dehydrogenase, 5' to roGF…

Expression

Mammalian

Mutation

C48S, Q80R, S147C, and Q204C in Clontech's G…

Promoter

CMV

Available since

Dec. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCDNA3-Flag MKK6

Plasmid

#13517

Depositor

Roger Davis

Article

Raingeaud et al Mol Cell Biol. 1996 Mar . 16(3):1247-55.

Insert

MKK6 (MAP2K6 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 25, 2007

Availability

Academic Institutions and Nonprofits only

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pLV[Exp]-Puro-CMV>{ARL13B_6gly_GFP_6Gly_R_GECO1}

Plasmid

#232880

Purpose

Expresses the ARL13B coupled to GFP and RGECO1 calcium sensor.

Depositor

Paul DeCaen

Article

DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

Insert

ADP-ribosylation factor-like protein 13B (ARL13B Human)

Use

Lentiviral

Tags

GFP and RGECO1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV BiP-Myc-KDEL-wt

Plasmid

#27164

Purpose

Mammalian expression of human Bip with Myc tag near the C-terminus, retaining the C-terminal KDEL ER localization sequence

Depositor

Ron Prywes

Article

Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

Insert

BiP (HSPA5 Human)

Use

Tags

KDEL and Myc

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pLVX-NPC1(WT)-FLAG

Plasmid

#164972

Purpose

Lentiviral vector for NPC1 expression in mammalian cells

Depositor

Roberto Zoncu

Article

Davis et al Dev Cell. 2020 Dec 7. pii: S1534-5807(20

Insert

NPC1 (NPC1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-4R

Plasmid

#112090

Purpose

Bacterial expression plasmid containing a GST tag with 4 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-4R (NCK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX SH3(2)-3R

Plasmid

#112091

Purpose

Bacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

SH3(2)-3R (NCK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Lac

Available since

Feb. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Plasmid

#115684

Purpose

NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

Depositor

David Suter

Article

Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

Insert

Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLXSN-EGFR

Plasmid

#65226

Purpose

Expression of EGFR in mammalian cells

Depositor

Axel Ullrich

Article

Obermeier et al EMBO J. 1996 Jan 2;15(1):73-82.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Oct. 25, 2016

Availability

Academic Institutions and Nonprofits only
pCCL/IFNB1-d2eGFP-3’UTR

Plasmid

#180232

Purpose

Reporter plasmid utilizing d2eGFP as a reporter gene. Expression is designed to mimick IFNB1

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Front Cell Infect Microbiol. 2022 Jan 18

Insert

d2eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

1 kb upstream of IFNB1 CDS

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-p300

Plasmid

#89094

Purpose

mammalian expression of full-length human p300 in pSG5

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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7xSMAD_RE::FLuc

Plasmid

#124531

Purpose

SMAD2 FLuc luciferase reporter

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

SMAD FLuc reporter

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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CA-0039 CMVTO-hIL10 (Fused Dimer)

Plasmid

#231187

Purpose

Fused human IL-10 dimer

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Fused human IL-10 dimer (IL10 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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HA Seh1L pRK5

Plasmid

#46331

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Seh1 (SEH1L Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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HA WDR24 pRK5

Plasmid

#46335

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

WDR24 (WDR24 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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HA Sec13 pRK5

Plasmid

#46332

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Sec13 (SEC13 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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pTag-BFP-C-h-Rab7L1-c-Myc

Plasmid

#79803

Purpose

Express TagBFP labeled human Rab7L1.

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab7L1 (RAB29 Human)

Use

Tags

TagBFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pPPI4-Strep-SNAP-PD-1

Plasmid

#223569

Purpose

Protein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Tags

Twin-Strep, SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUHD.NFATc1nuc

Plasmid

#40616

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1nuc (NFATC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Promoter

CMV2

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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