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AC_pTpEF2

Plasmid

#139938

Purpose

Diatom promoter

Depositor

Raffaela Abbriano

Article

Jaramillo-Madrid et al Pharmaceuticals (Basel). 2020 Dec 21;13(

Insert

Thalassiosira pseudonana EF2 promoter

Use

Synthetic Biology

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Truncated_pvc10-Cas9

Plasmid

#243734

Purpose

PVC structural operon - untargeted (truncated) tail fiber (Pvc13) - spike tip (Pvc10) loaded with Cas9 on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Truncated_pvc10-Cas9

Expression

Bacterial

Available Since

Sept. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_Cas9-Pvc10

Plasmid

#243735

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with Cas9 on NTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_Cas9-Pvc10

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_pvc8-Cas9

Plasmid

#243737

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike base (Pvc8) loaded with Cas9 on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_pvc8-Cas9

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-Ad5_pvc10-Cas9

Plasmid

#243733

Purpose

PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob - spike tip (Pvc10) loaded with Cas9 on CTD

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

pvc13-Ad5_pvc10-Cas9

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pFABs9

Plasmid

#241905

Purpose

Ferrulic acid biosensor - expresses sfGFP in response to substituted cinnamic acid

Depositor

Neil Dixon

Article

Berepiki et al ACS Synth Biol. 2020 Mar 20;9(3):576-589

Insert

sfGFP, ferC, ferA

Use

Synthetic Biology

Mutation

none

Available Since

Sept. 17, 2025

Availability

Academic Institutions and Nonprofits only

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EGFP-FGD2

Plasmid

#242831

Purpose

Encodes human FGD2 and N-terminal EGFP tag

Depositor

Cheng-han Yu

Article

Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

Insert

FGD2 (FGD2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

N/A

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pNDGG014

Plasmid

#231335

Purpose

BEVA Golden Gate cloning vector; Narrow host range plasmid. L1-pMB1-Gm-ELT3; pNDGG021, pOGG009, pNDGG007, pOGG013. GmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pQGG003

Plasmid

#231330

Purpose

BEVA Golden Gate cloning vector; BEVA2.0 Narrow host range plasmid with I-SceI counterselectable site for BpiI cloning; KmR/NmR.

Depositor

Barney Geddes

Article

Geddes et al Can J Microbiol. 2025 Jan 1;71:1-13. doi

Type

Empty backbone

Expression

Bacterial

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-DD-T6B-WT-YFP

Plasmid

#235145

Purpose

Expresses the inducible T6B peptide fused with DHFR and YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

DHFR-fused T6B peptide

Use

AAV

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-L7-6-FLAG-HA-T6B-WT-YFP

Plasmid

#235141

Purpose

Expresses the T6B peptide fused with YFP under the Purkinje cell-specific L7-6 promoter

Depositor

Giordano Lippi

Article

Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

Insert

T6B peptide

Use

AAV

Tags

FLAG/HA

Promoter

L7-6

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-TO-g0 (FLP-IN)

Plasmid

#236120

Purpose

Plasmid encoding g0 guide RNA under control of CMV promoter with two TetR binding sites, used to equalize plasmid masses for transfection

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

g0

Expression

Mammalian

Promoter

CMV promoter with two TetR binding sites

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW434 4x(ZNF35/ZNF35tar)-SFFV-GFP

Plasmid

#236143

Purpose

Fluorescent reporter encoding GFP under control of SFFV promoter with four ZNF35/ZNF35 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

EGFP

Expression

Mammalian

Promoter

SFFV promoter with four ZNF35/ZNF35 binding sites…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW435 4x(ZNF35/IKZF1tar)-SFFV-GFP

Plasmid

#236144

Purpose

Fluorescent reporter encoding GFP under control of SFFV promoter with four ZNF35/IKZF1 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

EGFP

Expression

Mammalian

Promoter

SFFV promoter with four ZNF35/IKZF1 binding sites…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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EW420 4x(ZNF35/ZNF250tarv3)-SFFV-GFP

Plasmid

#236139

Purpose

Fluorescent reporter encoding GFP under control of SFFV promoter with four ZNF35/ZNF250 binding sites upstream

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

EGFP

Expression

Mammalian

Promoter

SFFV promoter with four ZNF35/ZNF250 binding site…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2ATetP2AP - H

Plasmid

#229972

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains last ActB exon fused T2A-tet regulatory elemeny-P2A-puro and Hygro

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2ATetP2AP - H

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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psBIG1a ActBT2AmcherryP2AP -TagBFPH

Plasmid

#229971

Purpose

Harbor sgRNA+PAM sequence of ActinB gene for knockin using CRISPR and Homology Independent Targeted Integration. Contains Last ActB exon fused T2A-mCherry-P2A-puro and mTagBFPnls-T2A-Hygro

Depositor

Martin Pelosse

Article

Pelosse et al Synthetic Biology 2025.02.13

Insert

ActBT2AmcherryP2AP -TagBFPH

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only

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Halo-BASU-His

Plasmid

#218451

Purpose

E coli expression vector for Halo-BASU-His biotin ligase

Depositor

Thorsten Stafforst

Article

Hanswillemenke et al Nat Chem Biol. 2024 Jan 17. doi: 10.1038

Insert

HaloTag-BASU-His fusion protein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAS458-U6_CBA-mCherry

Plasmid

#195566

Purpose

Transient expression of mCherry; U6 with scaffold for sgRNA cloning in mammalian cells.

Depositor

Joanna Wysocka

Article

Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

Insert

mCherry

Expression

Mammalian

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pWN165

Plasmid

#215284

Purpose

Expresses sfGFP under the Anderson J23104 promoter with an RK2 backbone.

Depositor

Brian Pfleger

Article

Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

Insert

sfGFP

Tags

His

Expression

Bacterial

Promoter

J23104

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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