We narrowed to 5,035 results for: codon optimized

1. pIntegrase_5

   Plasmid

   #60577

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBEAST_J23101-amidase

   Plasmid

   #128135

   Purpose

   The cell-free adapted backbone, pBEAST, expressing the amidase enzyme gene (benzamid to benzoate) under control of the constitutive promoter J23101, and RBS B0032

   Depositor

   Jean-Loup Faulon

   Article

   Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:

   Insert

   amidase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23101

   Available since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. 3xFLAG-dCas9/pMXs-IG

   Plasmid

   #51258

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-T3-eSpCAS9-NG-pA

   Plasmid

   #131466

   Purpose

   Expresses eSpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

   Depositor

   Wataru Fujii

   Article

   Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

   Insert

   Codon optimized eSpCas9-NG
   

   Use

   CRISPR

   Tags

   Flag, NLS, and polyA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUCHR-osTIR1-IRES-GFP

   Plasmid

   #110661

   Purpose

   Lentiviral vector for stable expression of osTIR1 and selection with GFP

   Depositor

   Dmitriy Mazurov

   Article

   Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

   Insert

   osTIR1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   March 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL2622 (pCas7_Vch)

   Plasmid

   #200894

   Purpose

   Expresses human codon-optimized V. cholerae Cas7 from a CMV promoter.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   VchCas7
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMB22

   Plasmid

   #122244

   Purpose

   Lentivirus delivery for stable expression of AsCas12a-VPR

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Insert

   mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
   

   Use

   Lentiviral

   Tags

   NLS and VPR activator

   Expression

   Mutation

   Promoter

   EFs

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. JH03

   Plasmid

   #73512

   Purpose

   mKate2_PH pCFJ150 - contains C. elegans codon optimized mKate2

   Depositor

   Bob Goldstein

   Article

   Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

   Insert

   pmex-5::mKate2::PH::tbb-2
   

   Use

   Tags

   mKate2

   Expression

   Mutation

   Promoter

   mex-5

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEP4-DU422gp160

   Plasmid

   #100926

   Purpose

   Mammalian expression plasmid for Env from the DU422.1 HIV-1 isolate

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 (DU422.1) Env
   

   Use

   Tags

   CD5 leader sequence

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene

   Promoter

   CMV

   Available since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pETM6-C4-mCherry

    Plasmid

    #66530

    Purpose

    Expresses mCherry under the control of Mutant T7 Promoter C4

    Depositor

    Mattheos Koffas

    Article

    Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

    Insert

    mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon Optimized for E. coli

    Promoter

    Mutant T7 Promoter - C4

    Available since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCEP4-B41gp160

    Plasmid

    #123247

    Purpose

    Mammalian expression plasmid for Env from the B41 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (B41) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. C1-FT-mCitrine-EAAARx8-CaaX

    Plasmid

    #155224

    Purpose

    MPAct with increased search radius

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
    

    Use

    Tags

    mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA6 MCV LTco S239A

    Plasmid

    #112194

    Purpose

    Expression of MCPyV LT mutant (Ser 239 to Ala)

    Depositor

    Patrick Moore

    Article

    Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;11

    Insert

    MCPyV LT codon optimized
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1 FLAG-SIV-RCM-vpx

    Plasmid

    #115844

    Purpose

    Encodes codon optimized FLAG tagged SIVRCM-Vpx

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SIVRCM-Vpx
    

    Use

    Tags

    3x-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSBY3_ligD-HygR

    Plasmid

    #104625

    Purpose

    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD and hygromysin resistance gene.

    Depositor

    Sheng Yang

    Article

    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d

    Insert

    FnCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    FnCpf1 codon-optimized C.glutamicum

    Promoter

    hsp60

    Available since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-T3-SpCAS9-NG-pA

    Plasmid

    #131465

    Purpose

    Expresses SpCAS9-NG nuclease in mammalian cells. Used to modify genome of mouse embroys

    Depositor

    Wataru Fujii

    Article

    Fujii et al Sci Rep. 2019 Sep 9;9(1):12878. doi: 10.

    Insert

    Codon optimized SpCas9-NG
    

    Use

    CRISPR

    Tags

    Flag, NLS, and polyA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. JH04

    Plasmid

    #73513

    Purpose

    mRuby2_PH pCFJ150 - contains C. elegans codon optimized mRuby2

    Depositor

    Bob Goldstein

    Article

    Heppert et al Mol Biol Cell. 2016 Nov 7;27(22):3385-33

    Insert

    pmex-5::mRuby2::PH::tbb-2
    

    Use

    Tags

    mRuby2

    Expression

    Mutation

    Promoter

    mex-5

    Available since

    Feb. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCEP4-CH111gp160

    Plasmid

    #123268

    Purpose

    Mammalian expression plasmid for Env from the CH111 HIV-1 isolate

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (AD8) Env
    

    Use

    Tags

    CD5 leader peptide

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pIntegrase_8

    Plasmid

    #60579

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_8
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIntegrase_12

    Plasmid

    #60583

    Purpose

    plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Integrase_12
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pBAD

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge