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Showing: 6301 - 6350 of 16065 results

  2. MSP977

    Plasmid
    #65774
    Purpose
    Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135E mutation in Cas9
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65774-plasmid-map-sequence-id-112656

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  4. pJAK080

    Plasmid
    #167279
    Purpose
    CodA-based vector for insertion of DNA by recombination downstream of the pyrE gene in C. difficile
    Depositor
    Robert Fagan
    Article
    Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11
    Type
    Empty backbone
    Use
    E. coli - c. difficile shuttle vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    167279-plasmid-map-sequence-id-330742

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  5. pscAAV-Tyr

    Plasmid
    #135616
    Purpose
    Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus
    Depositor
    Lin He
    Article
    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e
    Insert
    EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    135616-plasmid-map-sequence-id-296005

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  11. L2-AA007

    Plasmid
    #185750
    Purpose
    Vector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.
    Depositor
    Peter Szoevenyi
    Article
    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph
    Inserts
    HygR2
    eGFP
    Use
    Tags
    membrane localization tag Lti6b
    Expression
    Plant
    Mutation
    Promoter
    Available since
    July 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    185750-plasmid-map-sequence-id-367858

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  12. pX330_Actb sgRNA / hSpCas9

    Plasmid
    #172832
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1of Actb (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Article
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Insert
    sgRNA targeting intron 1 of Actb under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172832-plasmid-map-sequence-id-340914

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  13. pUB-nHyPer2 (NLS:HyPer2:NLS)

    Plasmid
    #84730
    Purpose
    Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Insert
    HyPer2 targeted to nucleus
    Use
    Tags
    NLS (nuclear localisation signal and NLS (nuclear…
    Expression
    Plant
    Mutation
    Promoter
    ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84730-plasmid-map-sequence-id-180307

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  14. pTSSX-STC

    Plasmid
    #115668
    Purpose
    E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag
    Depositor
    David Kramer
    Article
    Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459
    Insert
    small tetraheme cytochrome c
    Use
    Tags
    Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…
    Expression
    Bacterial
    Mutation
    Promoter
    tac
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    115668-plasmid-map-sequence-id-228494

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  22. pIRESneo2 mycBioID-Tollip

    Plasmid
    #178054
    Purpose
    myc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.
    Depositor
    David Tumbarello
    Article
    Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:
    Insert
    Toll-Interacting Protein (TOLLIP Human)
    Use
    Tags
    mycBioID
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    178054-plasmid-map-sequence-id-350117

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  30. pSLPB2-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

    Plasmid
    #187945
    Purpose
    dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.
    Depositor
    Edda Schulz
    Article
    Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:
    Insert
    SpdCas9-tagRFPt-P2A-tagBFP
    Use
    Synthetic Biology; Piggybac
    Tags
    NLS, NLS + HA-tag, P2A, tagBFP, and tagRFPt
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    187945-plasmid-map-sequence-id-373705

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  32. pCas9-beta-mmr

    Plasmid
    #169241
    Purpose
    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations
    Depositor
    Brian Pfleger
    Article
    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:
    Inserts
    cas9
    beta
    mutL-E36K
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Changed glutamic acid 36 to lysine
    Promoter
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169241-plasmid-map-sequence-id-333575

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  45. pEGFP-Rab21-CCSS

    Plasmid
    #83424
    Purpose
    Mouse Rab21, mutation of the putative COOH-terminal prenylation motif (residues 218 and 219 from C to S). Generated from pEGFP-Rab21 by site-directed mutagenesis.
    Depositor
    Johanna Ivaska
    Article
    Pellinen et al J Cell Biol. 2006 Jun 5;173(5):767-80.
    Insert
    Rab21 (Rab21 Mouse)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Residues 218 and 219 from C to S (CCSS: TGCTGT -&…
    Promoter
    CMV
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83424-plasmid-map-sequence-id-166315

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  48. pCX-SponGee-Kras

    Plasmid
    #134777
    Purpose
    mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling
    Depositor
    Xavier Nicol
    Article
    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e
    Insert
    SponGee-Kras
    Use
    Tags
    FLAG and mRFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    134777-plasmid-map-sequence-id-266270

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Showing: 6301 - 6350 of 16065 results