We narrowed to 8,882 results for: plat

1. HP-Cas9hGem-Ade2-LEU2-LINEAR

   Plasmid

   #174839

   Purpose

   pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

   Depositor

   Zengyi Shao

   Article

   Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial and Yeast

   Promoter

   TDH3p

   Available Since

   Jan. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

   Plasmid

   #207760

   Purpose

   MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. SIGLE-bio-His

   Plasmid

   #53337

   Purpose

   Expresses full-length Sialic acid-binding Ig-like lectin 12 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   SIGLE (SIGLEC12 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. F2RL2-bio-His

   Plasmid

   #51877

   Purpose

   Expresses full-length Proteinase-activated receptor 3 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   F2RL2 (F2RL2 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. His-DNPH1

   Plasmid

   #203224

   Purpose

   For protein expression. Human DNPH1 is an N-glycosidase with specificity toward 5-hydroxymethyl uridine monophosphate. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   DNPH1 (DNPH1 Human)
   

   Tags

   His tag (with TEV cleavage site)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. TREML1-bio-His

   Plasmid

   #51857

   Purpose

   Expresses full-length Trem-like transcript 1 protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   TREML1 (TREML1 Human)
   

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR_RAF1_94

   Plasmid

   #111826

   Purpose

   Knockout Raf1

   Depositor

   Steve Shih

   Article

   Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

   Insert

   gRNA targeting RAF1 94-114 (RAF1 Human)
   

   Use

   CRISPR

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. FB007

   Plasmid

   #119705

   Purpose

   Promoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

   Depositor

   Jose Marcos

   Article

   Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

   Insert

   Promoter gpdA
   

   Use

   Synthetic Biology; Domestication of dna parts for…

   Promoter

   gpdA

   Available Since

   March 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR221-TAZ CA

   Plasmid

   #79504

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human TAZ S89A mutant CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   Multisite gateway entry clone

   Tags

   3xFLAG

   Mutation

   S89A

   Promoter

   no Promoter

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLKO.1-puro U6 N. meningitidis sgRNA BfuAI large stuffer

    Plasmid

    #86195

    Purpose

    U6 based expression of N meningitidis sgRNA

    Depositor

    Scot Wolfe

    Article

    Amrani et al Genome Biol. 2018 Dec 5;19(1):214. doi:

    Insert

    nmCas9 sgRNA cloning cassete
    

    Use

    Lentiviral

    Promoter

    U6

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLCHKO_HPRT1_exon3_2_Lb

    Plasmid

    #155054

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 3
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. TMED1-bio-His

    Plasmid

    #51832

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED1 (TMED1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. IR904: pMVP (L3-L2) pA; CMV::eGFP-P2A-TETa-pA

    Plasmid

    #121799

    Purpose

    pMVP L3-L2 entry plasmid, contains polyA + CMV-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven GFP-P2A-TETa downstream of gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    polyA + CMV::eGFP-P2A-TETa-polyA
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. FB029

    Plasmid

    #119713

    Purpose

    Promoter paf from Penicillium chrysogenum domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Promoter paf
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Promoter

    Promoter paf

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. FB030

    Plasmid

    #119714

    Purpose

    Terminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    Terminator paf
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCSD_2XNLS-SpCas9MT3-NLS-3XHA-2XNLS-NmdCas9-2XNLS

    Plasmid

    #107323

    Purpose

    Expresses SpCas9 (R1335K) fused to dNmCas9 in mammalian cells

    Depositor

    Scot Wolfe

    Article

    Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

    Insert

    SpCas9-NmCas9 fusion
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    SpCas9 (R1335K) is fused to nuclease dead NmCas9 …

    Promoter

    CMV IE94

    Available Since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPBpuro-mNG-eDHFR(69K6)-Tiam1

    Plasmid

    #172102

    Purpose

    Mammalian expression of Tiam1(DH-PH) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-Tiam1(DH-PH) (TIAM1 Human)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. Topo_SpCas9.tracr_LbCas12a.DR

    Plasmid

    #155049

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - LbCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and LbCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEN_TGmiR_Gb2-K

    Plasmid

    #25766

    Purpose

    Entry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Gb2 miR-shRNA (Gnb2 Mouse)
    

    Use

    Entry vector

    Tags

    GFP

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. FB005

    Plasmid

    #119678

    Purpose

    Coding sequence nptII from E. coli domesticated into pUPD2 with AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    geneticin resistance marker
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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