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A low-cost paper-based synthetic biology platform for analyzing gut microbiota and host biomarkers.
Takahashi MK, Tan X, Dy AJ, Braff D, Akana RT, Furuta Y, Donghia N, Ananthakrishnan A, Collins JJ
Nat Commun. 2018 Aug 21;9(1):3347. doi: 10.1038/s41467-018-05864-4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
110696B. fragilis 16S toehold switch sensorToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output
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110697B. thetaiotaomicron 16S toehold switch sensorToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output
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110698E. coli 16S toehold switch sensorToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output
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110699B. longum 16S toehold switch sensorToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output
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110700B. adolescentis 16S toehold switch sensorToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output
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110701B. breve 16S toehold switch sensorToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output
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110702E. rectale 16S toehold switch sensorToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output
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110703R. hominis 16S toehold switch sensorToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output
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110704C. difficile 16S toehold switch sensorToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output
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110705F. prausnitzii 16S toehold switch sensorToehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output
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110706B. fragilis mRNA toehold switch sensorToehold switch sensor to detect a species specific mRNA with GFP output
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110707B. thetaiotaomicron mRNA toehold switch sensorToehold switch sensor to detect a species specific mRNA with GFP output
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110708E. coli mRNA toehold switch sensorToehold switch sensor to detect a species specific mRNA with GFP output
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110709B. longum mRNA toehold switch sensorToehold switch sensor to detect a species specific mRNA with GFP output
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110710B. adolescentis mRNA toehold switch sensorToehold switch sensor to detect a species specific mRNA with GFP output
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110711B. breve mRNA toehold switch sensorToehold switch sensor to detect a species specific mRNA with GFP output
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110712E. rectale mRNA toehold switch sensorToehold switch sensor to detect a species specific mRNA with GFP output
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110713R. hominis mRNA species specific sensorToehold switch sensor to detect a species specific mRNA with GFP output
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110714C. difficile mRNA toehold switch sensorToehold switch sensor to detect a species specific mRNA with GFP output
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110715C. difficile toxin B mRNA toehold switch sensorToehold switch sensor to detect the tcdB mRNA from toxigenic C. difficile with GFP output
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110716F. prausnitzii mRNA toehold switch sensorToehold switch sensor to detect a species specific mRNA with GFP output
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110717Calprotectin S100A9 toehold switch sensorToehold switch sensor to detect Calprotectin S100A9 mRNA with GFP output
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111907CXCL5 toehold switch sensorToehold switch sensor to detect CXCL5 mRNA with GFP output
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111908IL-8 toehold switch sensorToehold switch sensor to detect IL-8 mRNA with GFP output
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111909Oncostatin M toehold switch sensorToehold switch sensor to detect Oncostatin M mRNA with GFP output
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