We narrowed to 3,747 results for: mak

1. pCAGGS-FuG-E

   Plasmid

   #67509

   Purpose

   This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.

   Depositor

   Kazuto Kobayashi

   Article

   Kato et al J Neurosci Methods. 2014 Apr 30;227:151-

   Insert

   Fusion Glycoprotein type E
   

   Use

   Lentiviral

   Tags

   Fusion protein of Vesicular stomatitis Indiana vi…

   Promoter

   CAGGS

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCas-pML-1B_6xHis-Cas12m

   Plasmid

   #192282

   Purpose

   MmCas12m fused to His-tag for protein purification

   Depositor

   John van der Oost

   Article

   Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

   Insert

   MmCas12m
   

   Use

   CRISPR

   Tags

   His-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EF1a-FLEx-ASAP4b-Kv-WPRE

   Plasmid

   #201032

   Purpose

   Expresses somatically enriched ASAP4b from the EF-1alpha promoter in a cre-dependent manner

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4e-Kv
   

   Use

   AAV

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-nEF Con/Fon hChR2(H134R)-EYFP

   Plasmid

   #55644

   Purpose

   Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

   Depositor

   Karl Deisseroth

   Article

   Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

   Insert

   ChR2(H134R)-EYFP
   

   Use

   AAV; Cre on/flp on chr2-eyfp

   Tags

   EYFP

   Expression

   Mammalian

   Promoter

   nEF

   Available Since

   Aug. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKIIa-hsChRmine-oScarlet-KV 2.1-WPRE

   Plasmid

   #183521

   Purpose

   Optogenetics

   Depositor

   Karl Deisseroth

   Article

   Kishi et al Cell. 2022 Feb 17;185(4):672-689.e23. do

   Insert

   hsChRmine-oScarlet-Kv2.1
   

   Use

   AAV

   Mutation

   H33R

   Promoter

   CaMKIIa

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLIB_GST-TEV-SRC(WT)

   Plasmid

   #223742

   Purpose

   Expression of recombinant protein for purification, codon optimized for Insect cells

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

   Insert

   Src Kinase (SRC Human)
   

   Tags

   GST-TEV

   Expression

   Insect

   Available Since

   March 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-hSyn-ASAP4b-Kv-WPRE

   Plasmid

   #201030

   Purpose

   Expresses somatically enriched ASAP4b in neurons, can be used to package AAV

   Depositor

   Michael Lin

   Article

   Evans et al Nat Methods. 2023 Jul;20(7):1104-1113. d

   Insert

   ASAP4b-Kv
   

   Use

   AAV

   Available Since

   July 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR-GAL4-UAS-huCD25- IRES-mCherry-PGK-BFP

   Plasmid

   #223211

   Purpose

   Lentiviral vector - mCherry reporter and huCD25 for Gal4DBDVP64 synNotch receptors with a constitutive BFP

   Depositor

   Wendell Lim

   Article

   Reddy et al Science. 2024 Dec 6;386(6726):eadl4793.

   Insert

   CD25 (IL2RA Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-nEF-Con/Foff 2.0-iC++-EYFP

   Plasmid

   #137156

   Purpose

   Intersectional viral expression of ic++-EYFP in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-iC++-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. mVenus-proα1(I)

    Plasmid

    #119845

    Purpose

    Expresses mouse Type I procollagen α1 chain (Col1a1) with mVenus replacing exon 2-3 retaining N-propeptide minor triple helix and N-terminal cleavage site

    Depositor

    Sergey Leikin

    Article

    Omari et al Proc Natl Acad Sci U S A. 2018 Oct 23;11

    Insert

    Type I procollagen α1 chain (Col1a1 Mouse)
    

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Col1a1 exons 2-3 replaced by fluorescent tag

    Promoter

    CMV

    Available Since

    Jan. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR-RFP entry

    Plasmid

    #192278

    Purpose

    Entry vector for cloning MmCas12m spacers. RFP flanked by MmCas12m CRISPR repeats can be digested out with BbsI.

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    RFP flanked by type V-M CRISPR repeat sequences
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PJ23119

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hnEF Coff/Fon hChR2(H134R)-EYFP

    Plasmid

    #55649

    Purpose

    Cre-off/Flp-on ChR2-EYFP under the short Ef1a promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre off/flp on chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    Short Ef1

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETDuet-1_6xHis-TEV-mCh-NDP52

    Plasmid

    #187829

    Purpose

    Plasmid for the expression and purification of 6xHis-TEV-mCh-NDP52 in E. coli Rosetta pLysS. Internal Reference: SMC1148

    Depositor

    Sascha Martens

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Tags

    6xHisTag, TEV, and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCas-MmdCas12m-CBE1

    Plasmid

    #192284

    Purpose

    MmdCas12m-CBE1 expressed under a constitutive promoter

    Depositor

    John van der Oost

    Article

    Wu et al Mol Cell. 2022 Dec 1;82(23):4487-4502.e7

    Insert

    MmdCas12m-CDA-UGI
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    D485A

    Promoter

    PJ23108

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5-FRT-TO-EGFR*-CUbo

    Plasmid

    #212821

    Purpose

    Constitutive or doxycycline-inducible expression of EGFR*-CUbo in mammalian cells

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    EGFR* (EGFR Human, Budding Yeast)
    

    Tags

    CUbo

    Expression

    Mammalian

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn Con/Foff hChR2(H134R)-EYFP

    Plasmid

    #55646

    Purpose

    Cre-on/Flp-off ChR2-EYFP under the Synapsin promoter

    Depositor

    Karl Deisseroth

    Article

    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

    Insert

    ChR2(H134R)-EYFP
    

    Use

    AAV; Cre on/flp off chr2-eyfp

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-Ef1a-Con/Foff 2.0-EYFP

    Plasmid

    #137162

    Purpose

    Intersectional viral expression of EYFP in cells expressing Cre AND NOT Flp

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    Con/Foff 2.0-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    EF1a

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-IRES-GFP ASXL1 (p.G646 Wfs *12) 3x FLAG

    Plasmid

    #81021

    Purpose

    Cancer-associated ASXL1 truncation mutant in retroviral vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.G646 Wfs *12 truncation

    Promoter

    5’LTR

    Available Since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pLIB_GST-TEV-SRC (Y530F)

    Plasmid

    #223743

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    Src Kinase (SRC Human)
    

    Tags

    GST-TEV

    Expression

    Insect

    Mutation

    Y530F constitutively active mutant

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV6-XL4 ASXL1 (p.G646 Wfs *12) 3x FLAG

    Plasmid

    #74245

    Purpose

    Cancer-associated ASXL1 truncation mutant in mammalian expression vector

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    ASXL1 (ASXL1 Human)
    

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    p.G646 Wfs *12 truncation

    Promoter

    CMV

    Available Since

    Dec. 21, 2016

    Availability

    Academic Institutions and Nonprofits only