We narrowed to 4,182 results for: biorxiv
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Plasmid#216408PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutationsDepositorInsertEfg1-ΔDBD-Y115S/Y132S/Y139S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 115, 132 and 139 to Serine, and …PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX-EF1a_NKX2-1_HD_160-223AA
Plasmid#221489PurposeNKX2-1 N-terminal and C-terminal truncation (homeodomain only) mutation lentiviral overexpressionDepositorInsertNKX2-1 (NKX2-1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationDeletion of 1-159AA N-terminal and 224-371AA C-te…PromoterEF1aAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-FKBP-GFP-OPTN
Plasmid#208866PurposeStably express GFP-tagged OPTN for CID induced mitophagyDepositorInsertOPTN (OPTN Human)
UseTagsFKBP-GFPExpressionMammalianMutationPromoterAvailable sinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-oScarlet-WPRE-BC9-pA
Plasmid#220773PurposeExpression of oScarlet for cell isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertoScarlert-WPRE-BC9
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC3-pA
Plasmid#220755PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC3
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP670-C1-Nir1-LNS2
Plasmid#220085Purposehigh affinity PA biosensor with far-red fluorophoreDepositorInsertPITPNM3 (PITPNM3 Human)
UseTagsiRFP670-GGSGGMExpressionMammalianMutationamino acids 613-897PromoterCMVAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAV-CAG-Sun1-GFP-WPRE-BC7-pA
Plasmid#220759PurposeExpression of Sun1GFP for nuclei isolation and Projection-TAG BC for multiplex projection tracingDepositorInsertSun1-GFP-WPRE-BC7
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL475
Plasmid#219503PurposeExpress Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in transDepositorInsertAde2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-REPORT (PGK) 12xHRE-NanoLuc
Plasmid#208575Purposehypoxic response element driven NanoLuc Lentiviral reporterDepositorInsertsNanoLuciferase
d2eGFP
12xHRE
UseLentiviral, Luciferase, and Synthetic BiologyTags3x SV40 NLS and PESTExpressionMammalianMutationPromoterAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-IRES-puro6 +gal4DBDHIFCAD pGalO linker
Plasmid#207172Purposeexpression plasmid for Gal4DBD-HIF-CAD expression GalO linkerDepositorInsertGal4DBD-HIF1a-CAD GalO linker
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-tet2puro-gal4DBD-HIFCAD
Plasmid#207173PurposeDox Inducible Expression Plasmid for gal4DBD-HIF-CADDepositorInsertGal4DBD-HIF1a-CAD
UseLentiviral; Dox inducibleTags2x MycExpressionMammalianMutationPromoterTRE3GAvailable sinceApril 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE5 CTFdeltaTA-FLAG
Plasmid#217726PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE5 (ADGRE5 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-536PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF3 CTF-FLAG
Plasmid#217696PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF3 (ADGRF3 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-752PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRE5 CTF-FLAG
Plasmid#217693PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRE5 (ADGRE5 Human)
UseTagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-530PromoterAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P/Q443P
Plasmid#216402PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56 and Glutamine 443 to Proli…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-A51P/A56P/Q70P/Q443P
Plasmid#216403PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q70P/Q443 to proline mutationsDepositorInsertEfg1-ΔDBD-A51P/A56P/Q70P/Q443P
UseTags6xHis-tagsExpressionBacterialMutationchanged Alanine 51, 56 and Glutamine 70, 443 to P…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y4S/Y8S/Y16S
Plasmid#216405PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutationsDepositorInsertEfg1-ΔDBD-Y4S/Y8S/Y16S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 4, 8 and 16 to Serine, and delet…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y64S/Y66S/Y69S
Plasmid#216406PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutationsDepositorInsertEfg1-ΔDBD-Y64S/Y66S/Y69S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 64, 66 and 69 to Serine, and del…PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y101S/Y104S/Y106S
Plasmid#216407PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutationsDepositorInsertEfg1-ΔDBD-Y101S/Y104S/Y106S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 101, 104 and 106 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
Efg1-ΔDBD-Y401S/Y404S/Y409S
Plasmid#216409PurposeExpresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutationsDepositorInsertEfg1-ΔDBD-Y401S/Y404S/Y409S
UseTags6xHis-tagsExpressionBacterialMutationchanged Tyrosine 401, 404 and 409 to Serine, and …PromoterAvailable sinceApril 17, 2024AvailabilityAcademic Institutions and Nonprofits only