We narrowed to 5,822 results for: pCas

1. pCAG/VPS34(R561D|R566D|K567D|K568D)

   Plasmid

   #232917

   Purpose

   Mammalian Expression

   Depositor

   James Hurley

   Insert

   VPS34 (PIK3C3 Human)
   

   Expression

   Mammalian

   Mutation

   R561D|R566D|K567D|K568D

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCAGGS puro GFP-REX1-IRES-mCherry

   Plasmid

   #232239

   Purpose

   Mammalian expression of REX1-GFP-IRES-mCherry

   Depositor

   Francisco Bustos

   Article

   Bandi et al HGG Adv. 2024 Oct 31;6(1):100378. doi: 1

   Insert

   REX1-GFP-IRES-mCherry (Zfp42 Mouse)
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Available Since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCAGEN-DV-IRES-myr-BFP (JDW 491)

   Plasmid

   #229834

   Purpose

   A CAGGS driven gateway compatible destination vector that contains an IRES-myristoylated-TagBFP for labeling the cell membrane

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCAGGS-Twin-strep-TEV-DGKδ2-DSAMD

   Plasmid

   #223721

   Purpose

   Express human diacylglycerol kinase delta 2- SAM domain deletion mutant (N-terminal TEV protease cleavable Twin-Strep-fusion protein) in mammalian cell

   Depositor

   Chiaki Murakami

   Insert

   DGKD (DGKD Human)
   

   Tags

   TEV cleavable site and Twin-Strep-tag

   Expression

   Mammalian

   Mutation

   deleted sterile alpha motif domain (deleted amino…

   Promoter

   CAG and chicken β-actin promoter

   Available Since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCA528 IST1 L328D (316-366)(C-Cys)

   Plasmid

   #193035

   Purpose

   Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L328D mutation that diminishes binding to MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L328D, Residues 316-366, Non-native C-terminal Cy…

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCA528 IST1 L355A (316-366)(C-Cys)

   Plasmid

   #193036

   Purpose

   Bacterial expression plasmid for IST1 (316-366) with C-terminal Cysteine for fluorescence polarization binding assays. Contains L355A mutation that diminishes binding to MIT domains.

   Depositor

   Wesley Sundquist

   Article

   Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Tags

   6xHis-Sumo

   Expression

   Bacterial

   Mutation

   L355A, Residues 316-366, Non-native C-terminal Cy…

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCA528 IST1, residues 316-366, C-Cys

   Plasmid

   #191234

   Purpose

   Bacterial expression for IST1 residues 316-366, contains non-native C-terminal Cysteine for labeling

   Depositor

   Wesley Sundquist

   Article

   Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

   Insert

   IST1 (IST1 Human)
   

   Tags

   HIS-SUMO

   Expression

   Bacterial

   Mutation

   Residues 316-366, contains nonnative C-terminus f…

   Promoter

   T7

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

   Plasmid

   #226989

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 1a to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

   Plasmid

   #226991

   Purpose

   pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b

   Depositor

   Benjamin Kleinstiver

   Article

   Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

   Insert

   SpCas9 gRNA 2a to create OPA1-del_exon5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A6-gRNA_(CJT88)

    Plasmid

    #226987

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A6)

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA A6 to create OPA1-R194G
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A7-gRNA_(CJT89)

    Plasmid

    #226986

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A7)

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA A7 to create OPA1-R194G
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

    Plasmid

    #226992

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 2b to create OPA1-del_exon5
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

    Plasmid

    #226990

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 1b to create OPA1-del_exon5
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC19-U6-SpCas9_gRNA-OPA1-createR194G-A9-gRNA_(CJT87)

    Plasmid

    #226988

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-R194G cell line via adenine base editing (target base in position A9)

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA A9 to create OPA1-R194G
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA

    Plasmid

    #211819

    Purpose

    lentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9, tracrRNA and blasticidin resistance gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U1a promoter and miniU6 promoter

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti_CHyMErA_U6(SpCas9_I-SceI)-(AsCas12_PacI)_PGK-puro

    Plasmid

    #189634

    Purpose

    Lentiviral expression of single SpCas9 and AsCas12a gRNAs for generating combinatorial CHyMErA 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    hU6 Cas9-Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti_mpx_SpCas9_h7SK(PacI)_U6(I-SceI)_PGK-puro_v2

    Plasmid

    #189632

    Purpose

    Lentiviral expression of dual SpCas9 gRNAs for generating combinatorial SpCas9 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK Cas9 gRNA cassette, hU6 Cas9 gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGGS-iRFP713(C15S/V256C)-P2A-mCherry

    Plasmid

    #198069

    Purpose

    Mammalian expression of iRFP713(C15S/V256C) and EGFP

    Depositor

    Michiyuki Matsuda

    Insert

    iRFP713(C15S/V256C)
    

    Tags

    P2A-mCherry

    Expression

    Mammalian

    Mutation

    C15S/V256C mutation

    Promoter

    CAG

    Available Since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCA528 SNX15 MIT residues 263-342

    Plasmid

    #180625

    Purpose

    Bacterial expression for SNX15 MIT residues 263-342. Has N-terminal HIS-SUMO tag. Internal ID:WISP21-14

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SNX15 (SNX15 Human)
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 263-342

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCA528 SPASTIN , F124D, residues 108-200

    Plasmid

    #180629

    Purpose

    Bacterial expression for SPASTIN MIT, residues 108-200. Has F124D mutation. Has N-term HIS-SUMO tag. Used for FP experiments. Internal ID: WISP21-12.

    Depositor

    Wesley Sundquist

    Article

    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75

    Insert

    SPASTIN
    

    Tags

    HIS-SUMO

    Expression

    Bacterial

    Mutation

    Residues 108-200, F124D

    Promoter

    T7

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge