We narrowed to 6,009 results for: pCas

Showing: 2541 - 2560 of 6009 results

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pCasper4-tubulin-IVS-Syn21-QSco-p10

Plasmid

#46132

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QSco

Expression

Insect

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pKS023 - pCAGGS-3XFLAG-mKate2-Pds5b-bpA

Plasmid

#156442

Purpose

For transient expression of mouse PDS5B tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse PDS5B (Pds5b Mouse)

Expression

Mammalian

Mutation

none

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

Plasmid

#114089

Purpose

CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

Insert

human codon optimized FnCas12a

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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BPK3258 - human expression plasmid for eSpCas9(1.1)

Plasmid

#101176

Purpose

Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

hSpCas9-eSpCas9(1.1)(K848A/K1003A/R1060A)

Tags

3x FLAG and NLS (SV40)

Expression

Mammalian

Mutation

K848A/K1003A/R1060A

Promoter

CMV

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

Plasmid

#134331

Purpose

CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized Nme2Cas9

Tags

NLS(SV40)-3xFLAG

Expression

Mammalian

Promoter

CAG

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

Plasmid

#115142

Purpose

CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

Depositor

Joseph Bondy-Denomy, Keith Joung, Benjamin Kleinstiver

Article

Marino et al Science. 2018 Sep 6. pii: science.aau517

Insert

human codon optimized Mb3Cas12a

Tags

NLS(nucleoplasmin) and 3xHA

Expression

Mammalian

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mNG-eDHFR(69K6)-G-alpha-q(L254A)

Plasmid

#178851

Purpose

Mammalian expression of Gαq(L254A) fused to mNeonGreen-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

mNeonGreen-eDHFR(69K6)-Gαq(L254A) (GNAQ Human)

Expression

Mammalian

Mutation

Gαq: C9S/C10S/Q209L/L254A

Promoter

CAG

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pBW1604_pCAG-PV1-PYL-L1-PhiC31-234C-BGHpA

Plasmid

#108798

Purpose

Expresses aa234 to C-terminus of PhiC31 integrase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

PhiC31 integrase fragment aa234 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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NFATc2-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75232

Purpose

CRISPR/Cas9 plasmid against human NFATc2

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75236

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBW808_pCAG-PV5-VloxP-loxN-F3-GFP-BGHpA

Plasmid

#87584

Purpose

PV5 for 3-input, 2-output Full Adder

Depositor

Wilson Wong

Article

Weinberg et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

EGFP

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA

Plasmid

#108849

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

nMag

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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NFATc1-CRISPR #2 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

Plasmid

#75237

Purpose

CRISPR/Cas9 plasmid against human NFATc1

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-GST-hFIP200(1363-1370Mut)-MBP

Plasmid

#190038

Purpose

Expression of FIP200 mutant

Depositor

James Hurley

Article

Shi et al Elife. 2020 Aug 10;9. pii: 59099. doi: 1

Insert

FIP200 (RB1CC1 Human)

Tags

GST and MBP

Expression

Mammalian

Mutation

1363 RDKDLIES 1370 to GSSGGSSG

Promoter

CMV

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pCSDest2-SpCas9-NLS-3XHA-NLS-Zif268

Plasmid

#69219

Purpose

Expresses wild type SpCas9 fused to Zif268 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

Zif268 (Egr1 Mouse)

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pCAGGS-mTagBFP2-T2A-sfGFP-iCre-ERT2

Plasmid

#128166

Purpose

Monomeric TagBFP2 and sfGFP-iCre-ERT2 recombinase (PAPGSTM N-terminus, unskippable linker)

Depositor

Mario Capecchi

Article

Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10

Insert

mTagBFP2-T2A-sfGFP-iCre-ERT2

Use

Cre/Lox

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA

Plasmid

#108845

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GAI

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-EPCAM-COMP5AP-AviTag-9xHis

Plasmid

#157257

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

EPCAM (EPCAM Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA

Plasmid

#108843

Purpose

Insert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminus

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Type

Empty backbone

Use

Synthetic Biology

Tags

GID1

Expression

Mammalian

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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PET-SpCas9-HF1-plus-NLS-6xHis

Plasmid

#207381

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

SpCas9-HF1-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

N497A, Q695A, Q926A, amino acids 1005-1013 replac…

Promoter

T7

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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