We narrowed to 9,521 results for: try

1. pET22b_PstS_1

   Plasmid

   #78198

   Purpose

   Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A197C)
   

   Expression

   Bacterial

   Mutation

   A197C (please see depositors comment below)

   Promoter

   T7

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pR-mTagBFP2

   Plasmid

   #228596

   Purpose

   Mammalian expression of mTagBFP2, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pR-mRuby3

   Plasmid

   #228609

   Purpose

   Mammalian expression of mRuby3, for MuSIC barcode construction

   Depositor

   Marc Birtwistle

   Article

   Lu et al ACS Synth Biol. 2025 May 16;14(5):1533-1

   Insert

   mRuby3
   

   Expression

   Mammalian

   Mutation

   We introduced some silent mutations to avoid esse…

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. mVenus-IQ6 WT

   Plasmid

   #198201

   Purpose

   FRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenus

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   MYO5A IQ domain (Myo5a Mouse)
   

   Tags

   mVenus

   Expression

   Mammalian

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

   Plasmid

   #20943

   Purpose

   3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

   Insert

   channelrhodopsin-2
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CaMKIIa

   Available Since

   July 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSecTag-ATX

   Plasmid

   #17839

   Depositor

   Steven Rosen

   Article

   Kanda et al Nat Immunol. 2008 Apr . 9(4):415-23.

   Insert

   Autotaxin (ENPP2 Human)
   

   Expression

   Mammalian

   Available Since

   April 29, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pCB24 pDEST crybb1:TagBFP

   Plasmid

   #195987

   Purpose

   Transgenesis backbone

   Depositor

   Christian Mosimann, Markus Affolter

   Article

   Kemmler et al Development. 2023 Mar 28:dev.201531. doi

   Type

   Empty backbone

   Use

   Other

   Available Since

   Feb. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21b-Caspase-3

   Plasmid

   #90087

   Purpose

   Expresses wild type caspase 3 in bacterial cells

   Depositor

   Clay Clark

   Article

   Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Wild type

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET22b_PstS_3

   Plasmid

   #78200

   Purpose

   Expression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensor

   Depositor

   Martin Webb

   Article

   Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62

   Insert

   pstS (A17C,I76G,A197C)
   

   Expression

   Bacterial

   Mutation

   A17C,I76G,A197C (please see depositor comment bel…

   Promoter

   T7

   Available Since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK-CMV-hNHERF2

    Plasmid

    #47801

    Purpose

    Expresses human NHERF2 in mammalian cells

    Depositor

    Randy Hall

    Article

    Lau et al Biochemistry. 2001 Jul 24;40(29):8572-80

    Insert

    NHERF2 (NHERF2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaTdT-39

    Plasmid

    #228980

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-39, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-39
    

    Tags

    6xHIS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCB59 pDEST crybb1:mKate2

    Plasmid

    #195986

    Purpose

    Transgenesis backbone

    Depositor

    Christian Mosimann, Markus Affolter

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Type

    Empty backbone

    Use

    Other

    Available Since

    Feb. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pME minprom KalTa4

    Plasmid

    #195964

    Purpose

    Gateway pME Gal4 variant

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    mouse beta-globin minimal promoter KalTA4 (Gal4)
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. KP501: pMVP (L5-L4) eYFP-P2A

    Plasmid

    #121714

    Purpose

    pMVP L5-L4 entry plasmid, contains eYFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eYFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eYFP-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-10xSunTag(22aa)

    Plasmid

    #215745

    Purpose

    Expresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkers

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    dCas9-10xSunTag
    

    Expression

    Mammalian

    Promoter

    chicken beta-actin promoter

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBS-MXT GIRK2

    Plasmid

    #20675

    Depositor

    Henry Lester

    Article

    Silverman et al J Biol Chem. 1996 Nov 29. 271(48):30524-

    Insert

    Mus musculus G protien coupled inward rectifier K+ channel 2 (Kcnj6 Mouse)
    

    Use

    Xenopus expression

    Tags

    3' UTR Xenopus B- globin and 5' UTR Xen…

    Mutation

    A369V

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENTR221-NR1H2

    Plasmid

    #79513

    Purpose

    MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

    Depositor

    Peter Jon Nelson

    Article

    Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

    Insert

    LXR BETA (NR1H2 Human)
    

    Use

    Multisite gateway entry clone

    Promoter

    no Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. ND4.2-Left TALE-G1397-N-dead-mCherry

    Plasmid

    #179683

    Purpose

    Expression of base editor in mammalian cells and for cell sorting

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N (E1347A)_1xUGI_P2A_mCherry_ATP5B 3'UTR
    

    Expression

    Mammalian

    Mutation

    E1347A

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pirespuro2-CTLA4-mEos2

    Plasmid

    #98285

    Purpose

    mammalian expression of CTLA4-mEos2

    Depositor

    Mike Heilemann

    Article

    Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

    Insert

    CTLA4 (CTLA4 Human)
    

    Tags

    mEos2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ220-Ultra

    Plasmid

    #176889

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d

    Insert

    AsCas12a
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Plant

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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