We narrowed to 1,429 results for: MiCy

1. pOmnibac_ZZ_linker_K963_GFP_SNAP

   Plasmid

   #159727

   Purpose

   Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

   Depositor

   Ahmet Yildiz

   Article

   Ferro et al Science. 2022 Jan 21;375(6578):326-331.

   Insert

   KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
   

   Use

   Tags

   GFP and SNAP tags and ZZ affinity tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR207 SARS-CoV-2 NSP3_nostop

   Plasmid

   #149306

   Purpose

   Gateway-compatible Entry vector, with insert of NSP3 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1. Does not contain stop codon.

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   NSP3 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   May 6, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pDONR207 SARS-CoV-2 E

   Plasmid

   #141273

   Purpose

   Gateway-compatible Entry vector, with insert of the envelope (E) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Has Service

   Cloning Grade DNA

   Insert

   E (E SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pDONR207 SARS-CoV-2 M

   Plasmid

   #141274

   Purpose

   Gateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Has Service

   Cloning Grade DNA

   Insert

   M (M SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAcBac1-Mb-sfGFP 150TAG

   Plasmid

   #197570

   Purpose

   Expression of sfGFP 150TAG with Methanosarcina barkeri (Mb) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

   Depositor

   Ryan Mehl

   Article

   Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

   Inserts

   sfGFP 150TAG
   Pyl-tRNA (4 copies)
   

   Use

   Tags

   V5-His6

   Expression

   Mammalian

   Mutation

   N150TAG

   Promoter

   CMV and U6/H1

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR207 SARS-CoV-2 ORF7A

   Plasmid

   #141276

   Purpose

   Gateway-compatible Entry vector, with insert of ORF7A gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

   Depositor

   Fritz Roth

   Article

   Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

   Insert

   ORF7A (ORF7a SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Use

   Gateway-compatible entry vector

   Tags

   Expression

   Mutation

   Many synonymous changes due to codon optimization

   Promoter

   Available since

   April 1, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4

   Plasmid

   #214137

   Purpose

   Insect cell expression of the human 20SCP proteasome assembly chaperones

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   POMP (POMP Human)
   PSMG1 (PSMG1 Human)
   PSMG2 (PSMG2 Human)
   PSMG3 (PSMG3 Human)
   PSMG4 (PSMG4 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOmnibac_ZZ_linker_ybbR_Tau

   Plasmid

   #159717

   Purpose

   Expresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tag

   Depositor

   Ahmet Yildiz

   Article

   Ferro et al Science. 2022 Jan 21;375(6578):326-331.

   Insert

   Microtubule-associated protein tau (MAPT Human)
   

   Use

   Tags

   ZZ affinity tag, ybbR tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBFC0687

   Plasmid

   #186692

   Purpose

   ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo

   Depositor

   Jennifer Doudna

   Article

   Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

   Inserts

   tnsB
   tnsC
   tniQ
   Cas12k
   Sh_2xBsaI_NT (Guide Stuffer) gRNA
   Tn7 transposon
   GmR+sfGFP ShDART Cargo
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLac

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

    Plasmid

    #169182

    Purpose

    Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp12-His6-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
    

    Use

    Tags

    His6-3xFlag

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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