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pRS424-TUB2 (1-428)

Plasmid

#60395

Purpose

Expression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoter

Depositor

Ron Vale

Article

Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

Insert

TUB2

Expression

Yeast

Mutation

deleted 429 - end

Promoter

GAL

Available Since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
MTS-ABE8e-Cas12

Plasmid

#251966

Purpose

Expression of ABE8e (with LbCpf1) in mitochondria for yeast mtDNA editing

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

ABE8e-LbCpf1

Use

CRISPR

Tags

MTS and V5-P2A-EGFP

Expression

Yeast

Promoter

GPD

Available Since

March 18, 2026

Availability

Academic Institutions and Nonprofits only

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pADH1-MTS-ABE8e-P2A-EGFP

Plasmid

#251973

Purpose

Yeast mtDNA editing with a mid-strength promoter for lower expression level

Depositor

Alice Ting

Article

Yin et al Proc Natl Acad Sci U S A. 2026 Jan 20;12

Insert

ABE8e

Use

CRISPR

Tags

MTS and V5-P2A-EGFP

Expression

Yeast

Promoter

ADH1

Available Since

Feb. 24, 2026

Availability

Academic Institutions and Nonprofits only

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yEPAC-R279L pDRF1-GW

Plasmid

#246454

Purpose

Expresses the non-responsive yEPAC-R279L sensor in budding yeast.

Depositor

Bas Teusink

Article

Botman et al Mol Biol Cell. 2021 Jun 15;32(13):1229-1

Insert

yEPAC-R269L

Expression

Yeast

Promoter

PMA1

Available Since

Oct. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-BirA-HA3-HIS3MX6

Plasmid

#227975

Purpose

To insert HA-tagged biotin ligase, BirA, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BirA

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CUP1p-TID-FRB-HA3

Plasmid

#228008

Purpose

Expresses TurboID-FRB fusion from CUP1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

CUP1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CUP1p-TID-FKBP-HA3

Plasmid

#228010

Purpose

Expresses TurboID-FKBP fusion from CUP1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FKBP

Tags

HA tag

Expression

Yeast

Promoter

CUP1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRS416-CHA1p-TID-FRB-HA3

Plasmid

#228012

Purpose

Expresses TurboID-FRB fusion from CHA1 promoter; yeast BioID

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

TurboID-FRB

Tags

HA tag

Expression

Yeast

Promoter

CHA1

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-BioID2-HA3-HIS3MX6

Plasmid

#227977

Purpose

To insert HA-tagged biotin ligase, BioID2, at the C-terminus of genes; yeast BioID, HIS3MX6 marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BioID3

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pFa6-BASU-HA3-HIS3MX

Plasmid

#227979

Purpose

To insert biotin ligase, BASU, at the C-terminus of genes; yeast BioID, HIS3MX marker

Depositor

Joseph Reese

Article

Pfannenstein et al G3 (Bethesda). 2024 Sep 13:jkae221. doi:

Insert

BASU

Tags

HA tag

Expression

Yeast

Available Since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

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