-
Plasmid#85892PurposeSensor PlasmidDepositorInsertmiR-218-1-3p Sensor (MIR218-1 Human)
UseTagsExpressionMutationPromoterPGKAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-615-3p.sensor.PGK.dNGFR.WPRE
Plasmid#85913PurposeSensor PlasmidDepositorInsertmiR-615-3p (MIR615 Human)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-214-3p.sensor.PGK.dNGFR.WPRE
Plasmid#85898PurposeSensor PlasmidDepositorInsertmiR-214-3p Sensor (MIR214 Human)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
AB.pCCL.sin.cPPT.GFP.miR-33a-3p.sensor.PGK.dNGFR.WPRE
Plasmid#85853PurposeSensor PlsmidDepositorInsertmiR-33a-3p Sensor (MIR33A Human)
UseLentiviralTagsExpressionMutationPromoterPGKAvailable sinceJan. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral
Plasmid#207507PurposeThis plasmid can be used to generate retrovirus.DepositorInserttNGFR, HA-GD2-28z_CAR (NGFR Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-EF1a-NGFP-mAID
Plasmid#140607PurposeExpression of OsTIR1 and GFP-mAID protein in mammalian cells under the control of EF1a promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
p34.pA.NGFR.mCMV.hPGK.mSrtA(Ge)TM.WPRE[sequenced]
Plasmid#226759Purposesortase A (GE) - TM in p36DepositorInsertsortase A (srtA )
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSGV Hu Acceptor PGK-NGFR
Plasmid#64270PurposeRetroviral golden gate cloning vector for expression of functional Human T-Cell Receptor genes with NGFRDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-2xMARS-nGFP-puro
Plasmid#205239PurposeDOX inducible expression of two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells from the Sleeping Beauty transposon systemDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseTransposonTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…Promotertight TRE promoterAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-LNGFR-recipient
Plasmid#11660Purpose3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expressionDepositorTypeEmpty backboneUseLentiviralTagsLNGFRExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1a-extGluc-NGFR
Plasmid#104832PurposeextGluc luciferase for invivo Imaging, NGFR for flourescent detection or separationDepositorInsertsextGLuc
NGFR
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-2xMARS-nGFP-puro
Plasmid#205238PurposeLentiviral vector encoding two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobodyDepositorInsert2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
UseLentiviralTagsHA, Nuclear Export Sequence, mScarlet-i, and nGFP…ExpressionMammalianMutationamino acids 143-271 of PLEKHA5 (GenBank reference…PromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTR-469 JUNB-tNGFR
Plasmid#186060PurposeKnockin of truncated NGFR to target geneDepositorInsertJUNB (JUNB Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTR-472 FOXO1-tNGFR
Plasmid#186062PurposeKnockin of truncated NGFR to target geneDepositorInsertFOXO1 (FOXO1 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPRIME-CMV-LNGFR-FF3
Plasmid#11666Purpose3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferaseDepositorInsertFF3
UseLentiviralTagsLNGFRExpressionMammalianMutationPromoterAvailable sinceMarch 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NGFP-VMAn-FKBP
Plasmid#70225PurposeExpresses N-terminal portion of GFP along with VMA intein for protein splicingDepositorInserteGFP
UseTagsFK506- and rapamycin-binding protein (FKBP), Link…ExpressionMammalianMutationChanged Glu 125 to Ile, Deleted amino acids 129-2…PromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIG-671_HA-GD2-28z_CAR_tNGFR
Plasmid#207484PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInserttNGFR, HA-GD2-28z_CAR (NGFR Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAID1.2-CMV-NGFP-mAID
Plasmid#140605PurposeExpression of OsTIR1 and GFP-mAID protein in DT40 cells under the control of CMV promoterDepositorInsertOsTIR1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTR-471 IRF4-tNGFR
Plasmid#186061PurposeKnockin of truncated NGFR to target geneDepositorInsertIRF4 (IRF4 Human)
UseCRISPRTagsExpressionMutationWT with tNGFR sequencePromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
MSGV Rh Acceptor PGK-NGFR
Plasmid#64272PurposeRetroviral golden gate cloning vector for expression of functional Rhesus T-Cell Receptor genes with NGFRDepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only