We narrowed to 4,416 results for: Pet2;

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pDL28_Cas9-His Δcys E532C/E1207C

Plasmid

#206289

Purpose

Bacterial expression of SpCas9 Δcys E532C and E1027C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S, E1207C

Promoter

T7

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL25_Cas9-His Δcys E1207C

Plasmid

#206286

Purpose

Bacterial expression of Cas9-His Δcys E1027C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, C574S, E1207C

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL23_Cas9-His Δcys E945C

Plasmid

#206284

Purpose

Bacterial expression of Cas9-His Δcys E945C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, C574S, E945C

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL24_Cas9-His Δcys E1026C

Plasmid

#206285

Purpose

Bacterial expression of Cas9-His Δcys E1026C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, C574S, E1026C

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL27_Cas9-His Δcys E532C/E945C

Plasmid

#206288

Purpose

Bacterial expression of SpCas9 Δcys E532C and E945C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S, E945C

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL26_Cas9-His Δcys M1C/E532C

Plasmid

#206287

Purpose

Bacterial expression of SpCas9 Δcys M1C and E532C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

M1C, C80S, E532C, C574S

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL22_Cas9-His Δcys E532C

Plasmid

#206283

Purpose

Bacterial expression of Cas9-His Δcys E532C variant under T7 promoter

Depositor

Amit Choudhary

Article

Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

Insert

SpCas9

Use

CRISPR

Tags

6x-His and SV40 NLS

Expression

Bacterial

Mutation

C80S, E532C, C574S

Promoter

T7

Available Since

Sept. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2572

Plasmid

#195683

Purpose

Expression/purification of 6xHis-TEV-MlaC(V171R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(V171R)

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1223

Plasmid

#195688

Purpose

Expression/purification of 6xHis-TEV-MlaDdelta173-183

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaDdelta173-183

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1909

Plasmid

#195690

Purpose

Expression/purification of 6xHis-TEV-MlaD(F118A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(F118A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1910

Plasmid

#195691

Purpose

Expression/purification of 6xHis-TEV-MlaD(L123A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaD(L123A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2555

Plasmid

#195673

Purpose

Expression/purification of 6xHis-TEV-MlaC(G170K)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(G170K)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2556

Plasmid

#195674

Purpose

Expression/purification of 6xHis-TEV-MlaC(I130D)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(I130D)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2559

Plasmid

#195675

Purpose

Expression/purification of 6xHis-TEV-MlaC(L76D)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(L76D)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2560

Plasmid

#195676

Purpose

Expression/purification of 6xHis-TEV-MlaC(N155A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(N155A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2561

Plasmid

#195677

Purpose

Expression/purification of 6xHis-TEV-MlaC(N179E)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(N179E)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2563

Plasmid

#195678

Purpose

Expression/purification of 6xHis-TEV-MlaC(R134E)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R134E)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2564

Plasmid

#195679

Purpose

Expression/purification of 6xHis-TEV-MlaC(R147D)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R147D)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2565

Plasmid

#195680

Purpose

Expression/purification of 6xHis-TEV-MlaC(R153A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R153A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2566

Plasmid

#195681

Purpose

Expression/purification of 6xHis-TEV-MlaC(R186A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(R186A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2568

Plasmid

#195682

Purpose

Expression/purification of 6xHis-TEV-MlaC(T176R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(T176R)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2573

Plasmid

#195684

Purpose

Expression/purification of 6xHis-TEV-MlaC(Y72A)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(Y72A)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2574

Plasmid

#195685

Purpose

Expression/purification of 6xHis-TEV-MlaC(Y82K)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(Y82K)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2576

Plasmid

#195686

Purpose

Expression/purification of 6xHis-TEV-MlaC(Y164R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(Y164R)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1224

Plasmid

#195687

Purpose

Expression/purification of 6xHis-TEV-MlaDdelta153-183

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaDdelta153-183

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2552

Plasmid

#195670

Purpose

Expression/purification of 6xHis-TEV-MlaC(A168R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(A168R)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2553

Plasmid

#195671

Purpose

Expression/purification of 6xHis-TEV-MlaC(D165I)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(D165I)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2554

Plasmid

#195672

Purpose

Expression/purification of 6xHis-TEV-MlaC(E169R)

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaC

Expression

Bacterial

Mutation

6xHis-TEV-MlaC(E169R)

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1160

Plasmid

#196025

Purpose

Expression/purification of 6xHis-TEV-MlaD

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1161

Plasmid

#196026

Purpose

Expression/purification of 6xHis-TEV-MlaDdelta141-183

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

MlaD

Expression

Bacterial

Mutation

6xHis-TEV-MlaDdelta141-183

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge