We narrowed to 3,506 results for: ttl

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pFW2000

Plasmid

#224211

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual-M2

Plasmid

#135584

Purpose

High eukaryotic dual protein expression shuttle vector

Depositor

Xiao-Wen Cheng

Article

Shang et al J Biotechnol. 2017 Aug 10;255:37-46. doi

Type

Empty backbone

Use

Transfer vector

Expression

Insect

Promoter

polyhedrin of AcMNPV

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pEC3105 (pSpy1C-PxylS2_ffluc)

Plasmid

#218512

Purpose

replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Mutation

deletion of Plac_mrfp cassette

Promoter

PxylS2

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-SERT-myc

Plasmid

#107456

Purpose

Mammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Serotonin Transporter (SERT) (SLC6A4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pAP114

Plasmid

#120799

Purpose

E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)

Depositor

Craig Ellermeier, David S. Weiss

Article

Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-

Insert

Pxyl::mCherryOpt

Expression

Bacterial

Mutation

1.4 kb xylR-Pxyl DNA fragment from C. difficile R…

Promoter

Pxyl

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pDSW1728

Plasmid

#120812

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of red fluorescent protein (mCherryOpt)

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al Appl Environ Microbiol. 2015 Mar;81(5):1

Insert

red fluorescent protein (mCherry), codon-optimized for C. difficile

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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hSERT-N1-pEYFP

Plasmid

#70105

Purpose

mammalian expression of hSERT with C-terminal YFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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hSERT-N1-pECFP

Plasmid

#70106

Purpose

mammalian expression of hSERT with C-terminal CFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Tags

ECFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only
pJYS1Ptac

Plasmid

#85545

Purpose

Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
recT

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Promoter

tac and unknown

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pJK02

Plasmid

#105133

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

Depositor

Joseph Sorg

Article

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJYDN3p

Plasmid

#162457

Purpose

Positive control plasmid for pJYDN3n plasmid expression attempts. It contains both eUnaG2 bilirubin dependent fluorescent protein and ALFA-tag for DnbALFA based detection on the cell surface.

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-eUnaG2 (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

ALFA tag, HA and myc tags, and eUnaG2 fluorescent…

Promoter

GAL1

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pSEVA-gRic6T

Plasmid

#106401

Purpose

E.coli-Pseudomonas putida KT2440 shuttle plasmid containing sgRNA targeting the KT2440 genome, and homologous arms.

Depositor

Sheng Yang

Article

Sun et al Microb Cell Fact. 2018 Mar 13;17(1):41.

Insert

sgRNA

Expression

Bacterial

Promoter

pj23119

Available Since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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pLX-TRV2

Plasmid

#180516

Purpose

Agrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and a polylinker

Depositor

Jose-Antonio Daros

Article

Aragones et al Biotechnol J. 2022 Mar 24:e2100504. doi:

Insert

Tobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker

Expression

Plant

Promoter

35S CaMV

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

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p201H Cas9

Plasmid

#59176

Purpose

Cas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
hph

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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p201N Cas9

Plasmid

#59175

Purpose

Cas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA Shuttle

Depositor

Wayne Parrott

Article

Jacobs et al BMC Biotechnol. 2015 Mar 12;15:16. doi:

Inserts

Cas9
nptII

Use

CRISPR

Promoter

2x35S and StUbi-3P

Available Since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pAD-sfGFP(Sp)-Pman

Plasmid

#133833

Purpose

Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

Depositor

Oscar Kuipers

Article

Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

Insert

sfGFP(Sp)

Use

E.coli/bacillus spp. shuttle vector

Expression

Bacterial

Promoter

Pman

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pJYS1Peftu

Plasmid

#85546

Purpose

Constitutive expression of FnCpf1 and recT in C.glutamitum, etfu promoter

Depositor

Sheng Yang

Article

Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

Inserts

Cpf1
recT

Use

CRISPR; Shuttle vector corynebacterium glutamicum…

Expression

Bacterial

Promoter

etfu and unknown

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pJAK112

Plasmid

#167280

Purpose

CodA-based mutagenesis vector for C. difficile

Depositor

Robert Fagan

Article

Fuchs et al Proc Natl Acad Sci U S A. 2021 Jun 22;11

Type

Empty backbone

Use

E. coli - c. difficile shuttle vector

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pRAN334

Plasmid

#120814

Purpose

E. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt)

Depositor

Craig Ellermeier, David S. Weiss

Article

Ransom et al J Bacteriol. 2014 Jun;196(12):2290-300.

Insert

cyan fluorescent protein, codon-optimized for low GC organisms

Expression

Bacterial

Promoter

Ptet

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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