We narrowed to 9,270 results for: Coli

1. pNH-TrxT-G-S10

   Plasmid

   #127829

   Purpose

   Production of recombinant human ribosomal protein S10

   Depositor

   Ivanhoe Leung

   Article

   Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

   Insert

   RPS10 (RPS10 Human)
   

   Use

   Tags

   His6 - Thioredoxin - TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pProEx-Htb-cytATL2-6Gly-sfGFP

   Plasmid

   #124065

   Purpose

   E. coli. exp. and purif. of X. laevis cytATL fragment fused to GFP in c-terminus.

   Depositor

   Tom Rapoport

   Article

   Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

   Insert

   cytATL-sfGFP
   

   Use

   Tags

   sfGFP

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAL829

   Plasmid

   #119697

   Purpose

   precursor slow-folding maltodextrin binding protein, MBP or malE, with mutations Y283D, G289C and V347C

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   slow-folding maltose ABC transporter periplasmic binding protein, malE, with mutations Y283D, G289C and V347C
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   mutations Y283D, G289C and V347C

   Promoter

   T5

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAL832

   Plasmid

   #119305

   Purpose

   precusor outer membrane protein A, ompA, truncated after amino acid 176

   Depositor

   Linda Randall

   Article

   Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

   Insert

   precursor outer membrane protein A, ompA, truncated after amino acid 176 including with mutations T95C and I153C
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   truncated after amino acid 176, mutations Threoni…

   Promoter

   lac

   Available since

   Jan. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-clbH-C-A2-PCP

   Plasmid

   #114157

   Purpose

   Expresses ClbH-C-A2-PCP

   Depositor

   Emily Balskus

   Article

   Zha et al Nat Chem Biol. 2017 Oct;13(10):1063-1065

   Insert

   clbH
   

   Use

   Tags

   6xHis-thrombin-T7 tag

   Expression

   Bacterial

   Mutation

   Only contains the C-A2-PCP domains of ClbH

   Promoter

   Available since

   Aug. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKDsgRNA-fsaA

   Plasmid

   #102286

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   fsaA gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKDsgRNA-adhE

   Plasmid

   #102290

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   adhE gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pKDsgRNA-ompT

   Plasmid

   #102291

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   ompT gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKDsgRNA-srlA

   Plasmid

   #89965

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting srlA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   srlA gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKDsgRNA-rbsK

    Plasmid

    #89963

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rbsK.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rbsK gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKDsgRNA-yhhT

    Plasmid

    #89958

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting yhhT.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    yhhT gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pKDsgRNA-torZ

    Plasmid

    #89956

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting torZ.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    torZ gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKDsgRNA-arsB

    Plasmid

    #89952

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting arsB.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    arsB gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBP-J23108

    Plasmid

    #72964

    Purpose

    Level 0 - J23108 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23108 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBP-J23114

    Plasmid

    #72965

    Purpose

    Level 0 - J23114 standard iGEM promoter (5' CTAT/3' GTAC fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    J23114 promoter
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBP-T7_RBS

    Plasmid

    #72978

    Purpose

    T7 promoter and RBS - derived from pET3a (5' CTAT/3' CATA) fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    T7+RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBP-Strep(II)

    Plasmid

    #72984

    Purpose

    Streptavidin(II) N-terminal tag (5' TAAA/3' CATA fusion)

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Strep(II) N-terminal tag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTU1-C-RFP

    Plasmid

    #72941

    Purpose

    Level 1 Destination vector - Position C

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 1 - C site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Beta-lactamase - G717C (nucleotide) - silent muta…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBP-lacZ

    Plasmid

    #72948

    Purpose

    Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 0 - Bioparts
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTU2-A-lacZ

    Plasmid

    #72950

    Purpose

    Level 2 Destination vector - Position A - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - A site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTU2-B-lacZ

    Plasmid

    #72951

    Purpose

    Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - B site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTU2-C-lacZ

    Plasmid

    #72952

    Purpose

    Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - C site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTU2-B-RFP

    Plasmid

    #72955

    Purpose

    Level 2 Destination vector - Position B - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - B site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pTU2-C-RFP

    Plasmid

    #72956

    Purpose

    Level 2 Destination vector - Position C - Assembly of 4-5 TU's from Level 1-ABCD or ABCDE

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - C site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTU2-b-RFP

    Plasmid

    #72959

    Purpose

    Level 2 Destination vector - Position B - Assembly of 3 TU's from Level 1-ABC

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 2 - b site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

26. Lf-MetQ

    Plasmid

    #78305

    Purpose

    Methionine Binding Protein (MetQ) with C-Terminal 6x Histidine Tag

    Depositor

    Douglas Rees

    Article

    Nguyen et al Biol Chem. 2015 Sep;396(9-10):1127-34. d

    Insert

    Methionine Binding Protein (metQ )
    

    Use

    Tags

    6x Histidine Tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pXY029 (mEos2-MTSBs)

    Plasmid

    #72652

    Purpose

    Inducible expression of mEos2-MTSBs in bacteria

    Depositor

    Jie Xiao

    Article

    Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

    Insert

    mEos2-MTSBs
    

    Use

    Tags

    mEos2

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. DVK

    Plasmid

    #66072

    Purpose

    MoClo Destination Vector: Empty vector for creating new DVK. Cut with SpeI and insert amplified LacZa (based on pSB1K3)

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Type

    Empty backbone

    Use

    Synthetic Biology; Destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. B0015_DE

    Plasmid

    #66035

    Purpose

    MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:E]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Double Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. B0015_DF

    Plasmid

    #66036

    Purpose

    MoClo Basic Part: Double terminator (B0010:B0012) [D:B0015:F]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Double Terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge