We narrowed to 2,972 results for: try
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Plasmid#228627PurposeMammalian expression of mOrange2 and extra insert, for MuSIC barcode constructionDepositorInsertmOrange2
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM-mRuby3
Plasmid#228628PurposeMammalian expression of mRuby3 and extra insert, for MuSIC barcode constructionDepositorInsertmRuby3
ExpressionMammalianMutationWe introduced some silent mutations to avoid esse…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
HTR5-DuET
Plasmid#213325PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
F2RL3-DuET
Plasmid#213237PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMN HA-ATG13(W50D)
Plasmid#188639PurposeFor expression of human ATG13(W50D)DepositorInsertATG13 (ATG13 Human)
UseRetroviralTagsHAExpressionMammalianMutationChanged tryptophan 50 to aspartic acidAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-D156R
Plasmid#188542PurposeGateway entry clone (attL1 & attR5) for LbCas12a-D156R mediated genome editingDepositorInsertLbCas12a-D156R
UseCRISPR; Gateway compatible lbcas12a-d156r entry c…TagsNLSExpressionPlantMutationD156RAvailable sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R1CiM_4EGFP_R3EGFPi2
Plasmid#185968PurposeMolecular IMPLY gate, encodes mutant Ci protein from Plteto-1 promoter, and EGFP form PR and PlTataa promoter. Must be expressed in E.coli DH5alphaz1 for experimental characterization.DepositorInsertMolecular IMPLY gate
UseSynthetic BiologyAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_R3tdTomatoi2
Plasmid#185969PurposeExpresses tdTomato from PR promoter with optimized RBS (i2) in E.coli Dh5alpha cell. The construct is in reverse direction network brick.DepositorInsertPr-i2rbs-tdTomato cassette in reverse direction in Network Brick
UseSynthetic BiologyPromoterPRAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
E1_pENTR_L1-L4_NI_G1333-DddtoxA-C
Plasmid#171724PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
E1_pENTR_L1-L4_NN_G1333-DddtoxA-C
Plasmid#171728PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of a cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
E3_pENTR_L3-L2_NG_G1333-DddtoxA-C
Plasmid#171732PurposeptpTALECD vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
E1_pENTR_L1-L4_NI_G1333-DddtoxA-N
Plasmid#171725PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
E1_pENTR_L1-L4_NN_G1333-DddtoxA-N
Plasmid#171729PurposeptpTALECD vector assembly, Entry clone 1 with attL1 and L4DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
E3_pENTR_L3-L2_NG_G1333-DddtoxA-N
Plasmid#171733PurposeptpTALECD vector assembly, Entry clone 1 with attL2 and L3DepositorInsertC- and N-termini of TALEN, half of cytidine deaminase domain of dddA of Burkholderia cenocepacia, uracil glycosylase inhibitor
UseMultisite gateway entry vectorAvailable sinceJuly 27, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYPQ287-RVR
Plasmid#138124PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized BsCas12a with N512R, K518V and N522R mutations, without promoterDepositorInsertBsCas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN512R, K518V and N522RAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ288 (BoCas12a)
Plasmid#138125PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized BoCas12a without promoterDepositorInsertBoCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ286 (CMaCas12a)
Plasmid#138122PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized CMaCas12a without promoterDepositorInsertCMaCas12a
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#920
Plasmid#110937PurposeExpresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animalDepositorInsertsmig-13 promoter
INS-22-Emerald
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
ExpressionBacterialAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
A102-GAP-BoCCS
Plasmid#123287PurposeExpression of truncated Glo3 (1-375) in E.ColiDepositorAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
A102-BoCCS-GRM
Plasmid#123288PurposeExpression of truncated Glo3 (137-459) in E.ColiDepositorAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only