We narrowed to 4,073 results for: p2a

1. pT22gCfpUbm5En2E4PCh(#251)

   Plasmid

   #184083

   Purpose

   cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-chkEn2-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT22Ubm5Eng2bE4PCh(#284)

   Plasmid

   #184088

   Purpose

   cyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-ZfEng2b-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pT22UbHEn2E4PCh(#262)

   Plasmid

   #184084

   Purpose

   cyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   3HA-chkEn2-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   3xHA

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pT22Ubm5Eng2aE4PCh(#283)

   Plasmid

   #184087

   Purpose

   cyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenic

   Depositor

   Sophie Vriz

   Article

   Rampon et al Development. 2015 May 15;142(10):1840-9.

   Insert

   5myc-ZfEng2a-ERT2-P2A-mCherry
   

   Use

   Zebrafish transgenesis (blue eyes)

   Tags

   5xMyc

   Expression

   Mutation

   ERT2: G400V, M543A, L544A, deleted 1-281;

   Promoter

   Zf-Ubi

   Available since

   June 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCW57-GFP-2A-MCS

   Plasmid

   #71783

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCW57-MCS1-2A-MCS2

   Plasmid

   #71782

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFETCh_Donor (EMM0021)

   Plasmid

   #63934

   Purpose

   3X Flag with P2A-Neo for 3' tagging of endogenous proteins

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Tags

   Glycine-Serine-3XFlag-P2A-NeoR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. GPR_control_lenti

   Plasmid

   #216223

   Purpose

   GFP P2A RFP control reporter in a lenti backbone

   Depositor

   Rebecca Voorhees

   Article

   Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

   Insert

   GFP P2A RFP_stop
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMF-TetTight

   Plasmid

   #193288

   Purpose

   Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

   Depositor

   Rudolf Jaenisch

   Article

   Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

   Insert

   rtTA-P2A-tTS
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF‐1α

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. GPR_control

    Plasmid

    #216222

    Purpose

    GFP P2A RFP control reporter

    Depositor

    Rebecca Voorhees

    Article

    Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

    Insert

    GFP P2A RFP_stop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. inert_EJC

    Plasmid

    #216224

    Purpose

    GFP P2A RFP reporter with inert EJC immediately following stop codon

    Depositor

    Rebecca Voorhees

    Article

    Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

    Insert

    GFP P2A RFP_inert_EJC
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTK095

    Plasmid

    #59560

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4 8xMS2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Endoplasmic reticulum-targeted optodPLD

    Plasmid

    #140059

    Purpose

    Encodes CRY2-mCherry-dPLD-P2A-CIBN-Sac1(TM) for mammalian expression

    Depositor

    Jeremy Baskin

    Article

    Tei et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Phospholipase D
    

    Use

    Tags

    CIBN, CRY2, P2A, Sac1(TM), and mCherry

    Expression

    Mammalian

    Mutation

    H170A (catalytic dead)

    Promoter

    Available since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTK333

    Plasmid

    #59574

    Purpose

    SIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5

    Depositor

    Ron Weiss

    Article

    Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

    Insert

    SGP(14) Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2 4xFF5
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSYC-181

    Plasmid

    #31566

    Depositor

    Seok-Yong Choi

    Article

    Kim et al PLoS One. 2011 . 6(4):e18556.

    Insert

    pCS4+-P2A
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCL390

    Plasmid

    #219500

    Purpose

    Integrating inducible cassette (Cas9-P2A-Csy4 and Eco1 RT) for yeast overexpression

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al bioRxiv [Preprint]. 2023 Jul 17:2023.07.

    Insert

    Integrating inducible cassette: Cas9-P2A-Csy4 and Eco1 RT
    

    Use

    Tags

    SV40-NLS

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-GAL10

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pT2iApowriter2(#336)

    Plasmid

    #184104

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transient transgenesis

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT2iU6Apowriter2N(#355)

    Plasmid

    #184106

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) by zebrafish transient transgenesis or mRNA synthesis

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transient transgenesis + in vitro trans…

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi+SP6

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT22Apowriter2(#340)

    Plasmid

    #184105

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    internal tag: 5myc

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT22iuasvApoWtEos3N(#486)

    Plasmid

    #184107

    Purpose

    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do

    Insert

    myr-DIAP1-2nls-tEosFP-nls'-P2A-Casp9-ERT2
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …

    Promoter

    4xUAS

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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