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pRZ150:CMVp-mK2-Ex1-miRS1-mK2-Ex2-PEST-miRS2-BSs-miRS3-BSs-pA

Plasmid

#251307

Purpose

Expression of mKate2 under CMVp promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mKate2

Expression

Mammalian

Promoter

CMVp

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pRZ171:CMVe-G3BSs-TATA-mK2-Ex1-miRS2-mK2-Ex2-miRS1-BSs-miRS3-BSs-pA

Plasmid

#251310

Purpose

Expression of mKate2 under CMVe promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mKate2

Expression

Mammalian

Promoter

CMVe

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR38:H2A1p-(linker2)-AFP BD-AS(50bp)mm(18,19)-(linker3)-(mutated BP Canonical 3'SE V3)-mK2-Ex2-(linker4)-pA

Plasmid

#251281

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR69:H2A1p-(linker2 [10bp])-AFP BD-AS(50bp)2mm(18-19)-(linker3)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251282

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR4:H2A1p-(linker2)-AFP BD-AS(50bp)2mm(18-19)-(linker3)-(AFP 3'SE)-mK2-Ex2-(linker4)-pA

Plasmid

#251265

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR5:H2A1p-(linker2)-AFP BD-AS(1-50bp)2mm(18-19)-(linker3)-(Canonical 3'SE V1)-mK2-Ex2-(linker4)-pA

Plasmid

#251266

Purpose

Trans-splicing expression of mK2-Ex2 under H2A1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex2

Expression

Mammalian

Promoter

H2A1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pKR14:SSX1p-mK2-Ex1-(5'D)-ID-AFP BD-S (50bp)-(linker5[20bp])-AFP BD-S (50bp, 30%GC)-(linker1)-pA

Plasmid

#251272

Purpose

Trans-splicing expression of mK2-Ex1 under SSX1p promoter for use in synthetic gene circuits

Depositor

Lior Nissim

Insert

mK2-Ex1

Expression

Mammalian

Promoter

SSX1p

Available Since

April 16, 2026

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_F113L

Plasmid

#248016

Purpose

Expression of synthetic Kemp eliminase (Des27.7 F113L)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_F113L

Tags

12xHis-bdSumo

Expression

Bacterial

Mutation

F113L

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_noCoreStab

Plasmid

#248014

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS&ActiveSite stability)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_noCoreStab

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_PROSS

Plasmid

#248012

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_PROSS

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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