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pMC-9-ccdB-11

Plasmid

#197813

Purpose

Level 0 J9-J11 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-ccdB-4

Plasmid

#197814

Purpose

Level 0 J1-J4 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-1-ccdB-11

Plasmid

#197815

Purpose

Level 0 J1-J11 destination vector, BbsI entry & CcdB selection

Depositor

Daniel Voytas

Article

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFH92

Plasmid

#125820

Purpose

Level0 nScCas9-ABE7.10, wheat/human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nScCas9-ABE7.10, wheat/human codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pUHD-SB-mAID/Bla

Plasmid

#171680

Purpose

TRE-controlled mAID-fusion sleeping beauty transposon vector with selection marker (blasticidin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Use

Tags

mAID

Expression

Mammalian

Mutation

Promoter

TRE

Available since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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C-12-BE

Plasmid

#135040

Purpose

C-T base editing using LjCDA1L2_1 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjCDA1L2_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCfB2224

Plasmid

#67547

Purpose

EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

Type

Empty backbone

Use

Cre/Lox; Integrative

Tags

Expression

Yeast

Mutation

Promoter

Available since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-TarHom-Tol2-TetR-cryaa-YFP

Plasmid

#73202

Purpose

BAC targeting cassette for pTARBAC2 with tetR selection marker and lens-specific transgenesis marker Citrine

Depositor

Holger Knaut

Article

Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

Insert

YFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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N-8-BE

Plasmid

#135023

Purpose

C-T base editing using LpCDA1L1_1 fused to N terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LpCDA1L1_1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFH52

Plasmid

#128181

Purpose

Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFH69

Plasmid

#128189

Purpose

Level1 ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPTK013-3a-Invertase-αMFΔ

Plasmid

#84972

Purpose

Type 3a, Invertase followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Invertase followed by αMFΔ secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
C-1-BE

Plasmid

#135029

Purpose

C-T base editing using PmCDA1L1_4 fused to C terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

PmCDA1L1_4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pPTK016-3-RFP_Intra

Plasmid

#84975

Purpose

Type 3, Red fluorescent protein

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Red fluorescent protein

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

BsaI site removed (1677 c to g and 1794 a to g)

Promoter

Non

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pFH37

Plasmid

#126011

Purpose

Level0 Rice OsU6-2 Pol III promoter with transcription Start-G

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Rice OsU6-2 Pol III promoter with transcription Start-G

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pUHD-SB-AID/Bla

Plasmid

#171678

Purpose

TRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (blasticidin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Use

Tags

AID

Expression

Mammalian

Mutation

Promoter

TRE

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUHD-SB-AID/Hyg

Plasmid

#171677

Purpose

TRE-controlled AID-fusion sleeping beauty transposon vector with selection marker (hygromycin)

Depositor

Randy Poon

Article

Yeung et al Mol Biol Cell. 2021 May 12:mbcE21020051.

Type

Empty backbone

Use

Tags

AID

Expression

Mammalian

Mutation

Promoter

TRE

Available since

July 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPTK014-3a-Killer-αMFΔ

Plasmid

#84973

Purpose

Type 3a, Killer followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Killer followed by αMFΔ secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pPTK011-3a-SA-αMFΔ

Plasmid

#84970

Purpose

Type 3a, Serum albumin followed by αMFΔ - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Serum albumin followed by αMFΔ secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
N-4-BE

Plasmid

#135019

Purpose

C-T base editing using LjCDA1L1_4 fused to N terminal of nCas9

Depositor

Zilong Qiu, Tian-Lin Cheng

Article

Cheng et al Nat Commun. 2019 Aug 9;10(1):3612. doi:

Insert

LjCDA1L1_4

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6 with EF-1alpha intron

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge