3,636 results

1. pGEX-4T-1-Staufen

   Plasmid

   #171545

   Purpose

   Encoding part of the Drosophila Staufen protein from M571 to P656

   Depositor

   Yan Zeng

   Article

   Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   Staufen (stau Fly)
   

   Expression

   Bacterial

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKMyc-fly DGCR8

   Plasmid

   #171561

   Purpose

   Encodes fly DGCR8 protein

   Depositor

   Yan Zeng

   Insert

   Pasha(CG1800) (pasha Fly)
   

   Expression

   Mammalian

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TetO-FUW-FLAG-NLS-EnR-MCS_N-term

   Plasmid

   #136884

   Purpose

   Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

   Depositor

   Moritz Mall

   Article

   Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

   Insert

   engrailed repressor domain (en Fly)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TetO-FUW-MCS-EnR_C-term

   Plasmid

   #136885

   Purpose

   Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon

   Depositor

   Moritz Mall

   Article

   Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

   Insert

   engrailed repressor domain (en Fly)
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Available Since

   Aug. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET-DEST42-dre4

   Plasmid

   #170440

   Purpose

   Express drosophila dre4 in E.coli expression system. Generated from pDONR-dre4 by Gateway system. Used for custom antibody purification.

   Depositor

   Joan Conaway

   Article

   Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

   Insert

   dre4 (dre4 Fly)
   

   Tags

   6xHis and V5

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-20

   Promoter

   T7

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-DEST42 -SSRP1

   Plasmid

   #170439

   Purpose

   Express drosophila SSRP1 in E.coli expression system. Generated from pDONR-SSRP1 by Gateway system. Used for custom antibody purification.

   Depositor

   Joan Conaway

   Article

   Tettey et al Cell Rep. 2019 Jun 25;27(13):3770-3779.e

   Insert

   ssrp1 (Ssrp Fly)
   

   Tags

   6xHis and V5

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3xFLAG-CMV-fly Drosha

   Plasmid

   #171560

   Purpose

   Encodes fly Drosha protein

   Depositor

   Yan Zeng

   Insert

   drosha(CG8730) (drosha Fly)
   

   Expression

   Mammalian

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAc5.1B-EGFP-DmTral_394-543-V5His6_D

   Plasmid

   #146134

   Purpose

   Insect Expression of DmTral_394-543

   Depositor

   Elisa Izaurralde

   Article

   Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

   Insert

   DmTral_394-543 (tral Fly)
   

   Expression

   Insect

   Mutation

   two silent mutations (nucleotides A198C, A387G) a…

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc5.1B-EGFP-DmBelle_1-298_B

   Plasmid

   #145913

   Purpose

   Insect Expression of DmBelle_1-298

   Depositor

   Elisa Izaurralde

   Insert

   DmBelle_1-298 (bel Fly)
   

   Expression

   Insect

   Mutation

   del 158-160 (GGG)

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAc5.1C-Fluc-CG7785_A

    Plasmid

    #145863

    Purpose

    Insect Expression of CG7785-3UTR

    Depositor

    Elisa Izaurralde

    Insert

    CG7785-3UTR (CG7785 Fly)
    

    Expression

    Insect

    Mutation

    317bp deletion in 3'UTR region (4172-4273bp)…

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAc5.1C-Fluc-nerfin_E

    Plasmid

    #146190

    Purpose

    Insect Expression of nerfin-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

    Insert

    nerfin-3UTR (nerfin-1 Fly)
    

    Expression

    Insect

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-EGFP-DmUPF2-siRNAres-V5His6_E

    Plasmid

    #146166

    Purpose

    Insect Expression of DmUPF2-siRNAres

    Depositor

    Elisa Izaurralde

    Insert

    DmUPF2-siRNAres (Upf2 Fly)
    

    Expression

    Insect

    Mutation

    3 non silent mutations: L1022P, V1097L and N1213S…

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTR(EF)-tRNA(Q)

    Plasmid

    #170517

    Purpose

    PCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Insert

    gRNAcore(EF)-tRNA(Q)
    

    Use

    Pcr template vector for amplifying grnacore(ef)-t…

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-lambdaN-HA-DmTral-LSm-R21EE23KR42EF44A-V5His6_C

    Plasmid

    #146071

    Purpose

    Insect Expression of DmTral-LSmR21EE23KR42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KR42EF44A (tral Fly)
    

    Expression

    Insect

    Mutation

    R21E, E23K, R42E, F44A. Additionally, one silent …

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc5.1B-EGFP-DmTral-R42EF44A_C

    Plasmid

    #146061

    Purpose

    Insect Expression of DmTral-R42EF44A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-R42EF44A (tral Fly)
    

    Expression

    Insect

    Mutation

    R42E, F44A. Additionally, two silent mutations A1…

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAc5.1B-EGFP-DmTral-LSm-R21EE23KS13AI15A-V5His6_C

    Plasmid

    #146049

    Purpose

    Insect Expression of DmTral-LSmR21EE23KS13AI15A

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

    Insert

    DmTral-LSmR21EE23KS13AI15A (tral Fly)
    

    Expression

    Insect

    Mutation

    S13A, I15A, R21E, E23K, one silent mutation A198C…

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAc5.1B-lambdaN-HA-DmTral-FDF-ADA_C

    Plasmid

    #146039

    Purpose

    Insect Expression of DmTral-FDF-ADA

    Depositor

    Elisa Izaurralde

    Article

    Tritschler et al Mol Cell. 2009 Mar 13. 33(5):661-8.

    Insert

    DmTral-FDF-ADA (tral Fly)
    

    Expression

    Insect

    Mutation

    FDF to ADA (407-409) mutation. Additionally, thre…

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1C-5Box-7-Fluc-STOP-CG10011-SV40_D

    Plasmid

    #146156

    Purpose

    Insect Expression of 5BoxB-7-Fluc-CG10011-3UTR

    Depositor

    Elisa Izaurralde

    Article

    Eulalio et al RNA. 2009 Jan . 15(1):21-32.

    Insert

    5BoxB-7-Fluc-CG10011-3UTR
    

    Expression

    Insect

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-EGFP-DmMe31B_268-459_D

    Plasmid

    #146119

    Purpose

    Insect Expression of DmMe31B_268-459

    Depositor

    Elisa Izaurralde

    Article

    Haas et al J Cell Biol. 2010 Apr 19. 189(2):289-302

    Insert

    DmMe31B_268-459 (me31B Fly)
    

    Expression

    Insect

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAc5.1B-lambdaN-DmPABPC1-V5His6_D

    Plasmid

    #146095

    Purpose

    Insect Expression of DmPABPC1

    Depositor

    Elisa Izaurralde

    Insert

    DmPABPC1 (pAbp Fly)
    

    Expression

    Insect

    Mutation

    seven silent mutations compared to the sequence g…

    Available Since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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