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pFA6a-6xGLY-HSV-HIS3MX6

Plasmid

#20774

Depositor

Mark Hochstrasser

Publication

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-VSV-G-kanMX6

Plasmid

#20772

Depositor

Mark Hochstrasser

Publication

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pNC952

Plasmid

#20401

Depositor

Beverly Errede

Publication

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP-reporter

Expression

Yeast

Mutation

X=UbiM (see schematic) linker=deltak

Available since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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pNC1053

Plasmid

#20406

Depositor

Beverly Errede

Publication

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP

Expression

Yeast

Mutation

linker: delta k

Available since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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pNC861

Plasmid

#20391

Depositor

Beverly Errede

Publication

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP-reporter

Expression

Yeast

Mutation

X=UbiY (see schematic) linker: ek

Available since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

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pT-59

Plasmid

#16089

Depositor

Yoshiko Kikuchi

Publication

Takahashi et al J Biochem (Tokyo). 2003 Apr . 133(4):415

Insert

AOS1 (AOS1 Budding Yeast)

Tags

GST

Expression

Bacterial

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pT-61

Plasmid

#16091

Depositor

Yoshiko Kikuchi

Publication

Takahashi et al J Biochem (Tokyo). 2003 Apr . 133(4):415

Insert

NFI1 (NFI1 Budding Yeast)

Tags

His and T7

Expression

Bacterial

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pBBK02 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitKanR, AmpR)

Plasmid

#178986

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitKanamycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pBBK06 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitNatR,AmpR)

Plasmid

#178990

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitNourseothrycin resistance

Depositor

Vincent Martin

Publication

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pJZC519

Plasmid

#62278

Purpose

Yeast dCas9-VP64 expression plasmid

Depositor

Wendell Lim, Stanley Qi

Publication

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

dCas9-VP64

Tags

Has a VP64 fusion

Expression

Yeast

Mutation

Has mutations in the RuvC1 and HNH domains to ren…

Promoter

pTdh3

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pNX3a-PK9

Plasmid

#78356

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

9xPK
kanMX6

Expression

Yeast

Available since

March 10, 2017

Availability

Academic Institutions and Nonprofits only

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pNX3s2-PK3

Plasmid

#78394

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xPK
zeoCV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3s2-FL3

Plasmid

#78395

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
zeoCV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-PK3

Plasmid

#78375

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xPK
ura4+ (ura4 Fission Yeast)

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-PK6

Plasmid

#78376

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

6xPK
ura4+ (ura4 Fission Yeast)

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-PK9

Plasmid

#78377

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

9xPK
ura4+ (ura4 Fission Yeast)

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3d-PK12

Plasmid

#78378

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

12xPK
ura4+ (ura4 Fission Yeast)

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3a2-PK3

Plasmid

#78382

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xPK
neoCV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3a2-FL3

Plasmid

#78383

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
neoCV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNX3a2-HA3

Plasmid

#78384

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Publication

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA
neoCV

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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