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pDZ306 (GLT1 POL1 24xPP7SL)
(Plasmid #35196)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 35196 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GLT1 POL1 24xPP7SL
  • Species
    Synthetic

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid

Depositor Comments

This plasmid was designed to construct mRNAs that contain multiple copies of an RNA sequence (the 24xPP7 stem-loops) that can be recognized by a chimeric RNA-binding protein fused to GFP, encoded in plasmid pDZ276 (Addgene plasmid #35194). See associated publication for detailed methods and use of this plasmid.

Please note that the LacZa-ccdB fusion in this plasmid is not functional and was originally present in the vector backbone to aid in screening for insert.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDZ306 (GLT1 POL1 24xPP7SL) was a gift from Robert Singer (Addgene plasmid # 35196 ; http://n2t.net/addgene:35196 ; RRID:Addgene_35196)
  • For your References section:

    Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson DR, Zenklusen D, Wu B, Chao JA, Singer RH. Science. 2011 Apr 22;332(6028):475-8. 10.1126/science.1202142 PubMed 21512033